Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 977,876 T→A 8.4% intergenic (+39/‑141) cysE → / → iscR serine acetyltransferase/DNA‑binding transcriptional regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947977,8760TA 8.4% 139.9 / 4.5 50intergenic (+39/‑141)cysE/iscRserine acetyltransferase/DNA‑binding transcriptional regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01

CAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCATGCGAC  >  NC_002947/977735‑978023
                                                                                                                                             |                                                                                                                                                   
cAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCACGCTGCCCAGCGCGACACTCAGCCGCATTGATCTCGCCTCACGGAAGTCACGGTGGCATGCGGGGCGTGTGCTCACGc                                                                                                                                             >  1:248389/1‑150 (MQ=255)
cAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGc                                                                                                                                             >  1:374848/1‑150 (MQ=255)
  aGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGccc                                                                                                                                           <  1:856067/150‑1 (MQ=255)
  aGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGccc                                                                                                                                           >  2:352347/1‑150 (MQ=255)
   ggtGGGCAGTGCCTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGCCACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGctcc                                                                                                                                          <  2:228213/148‑1 (MQ=255)
   gATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGAGCACACGcccc                                                                                                                                          <  1:816589/150‑1 (MQ=255)
      gggCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGAGCACACGccccgca                                                                                                                                       <  2:1098000/150‑4 (MQ=255)
        gCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTc                                                                                                                                     <  2:131019/150‑1 (MQ=255)
            tGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCt                                                                                                                                 <  2:742553/150‑1 (MQ=255)
                  ttGTGCCAAGGAACTGCCGGCCTTCCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGCGCTCACTCCCCTCGTCTGCTACAATc                                                                                                                           <  2:562226/150‑1 (MQ=255)
                       ccAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGc                                                                                                                                  >  1:444324/1‑138 (MQ=255)
                       ccAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGc                                                                                                                                  <  2:444324/138‑1 (MQ=255)
                         aaGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATc                                                                                                                           >  1:833817/1‑143 (MQ=255)
                         aaGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATc                                                                                                                           <  2:833817/143‑1 (MQ=255)
                                tGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCt                                                                                                             >  1:236182/1‑150 (MQ=255)
                                   cGGCCTTGCCGGATGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGAt                                                                                                          <  2:178454/150‑1 (MQ=255)
                                       cTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCaa                                                                                                      >  2:898271/1‑150 (MQ=255)
                                           ccGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACCATCTGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATccc                                                                                                  <  1:345395/150‑1 (MQ=255)
                                             ggaggaCGACTTCAGCGAGGCTGCCCAAGTGGCCCAACGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGa                                                                                                >  1:598990/1‑150 (MQ=255)
                                                  acgacTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTaaa                                                                                           <  1:766992/150‑1 (MQ=255)
                                                       ttCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTccc                                                                                                              <  1:614164/126‑1 (MQ=255)
                                                       ttCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTcca                                                                                                              >  2:614164/1‑125 (MQ=255)
                                                         cAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAg                                                                                    <  2:499583/150‑1 (MQ=255)
                                                           gCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGt                                                                                  <  1:699788/150‑1 (MQ=255)
                                                             gAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTaaa                                                                                           <  2:1115274/139‑1 (MQ=255)
                                                             gAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTaaa                                                                                           >  1:1115274/1‑139 (MQ=255)
                                                                   gCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCCCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGt                                                                          >  2:23169/1‑150 (MQ=255)
                                                                      cAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGa                                                                       >  2:513503/1‑150 (MQ=255)
                                                                      cAAGTGGCCCAACGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGa                                                                       <  2:598990/150‑1 (MQ=255)
                                                                             cccAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAAt                                                                <  2:374848/150‑1 (MQ=255)
                                                                              ccAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATg                                                               >  2:667130/1‑150 (MQ=255)
                                                                                 gcgcGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTc                                                            <  2:248389/150‑1 (MQ=255)
                                                                                        acTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGa                                                                                                           <  2:369700/96‑1 (MQ=255)
                                                                                        acTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGa                                                                                                           >  1:369700/1‑96 (MQ=255)
                                                                                             gCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACCATCGTGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATa                                                <  1:352347/150‑1 (MQ=255)
                                                                                              ccGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATa                                                                            <  1:80195/121‑1 (MQ=255)
                                                                                              ccGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATa                                                                            >  2:80195/1‑121 (MQ=255)
                                                                                                   ttGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGTAATCCCGACTAAAACAGCAGGTCTAAACGCTGACGTCAATTCTCGGGAATCGCATagtagcc                                          >  2:505386/1‑144 (MQ=255)
                                                                                                   ttGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAAGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGca                                          <  2:741524/150‑1 (MQ=255)
                                                                                                   ttGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAACAGCAGGTCTAATAGTTGTCTTAAATGCTCGGTAATCACATAATCGcc                                          >  2:505317/1‑149 (MQ=255)
                                                                                                         gcgcCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATc                                    <  1:493606/150‑1 (MQ=255)
                                                                                                           gccgcACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCt                                  >  2:575339/1‑150 (MQ=255)
                                                                                                                    aaGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACACTCTCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTATTGTTTGATTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTccc                         <  1:869362/150‑1 (MQ=255)
                                                                                                                       tCATGGTGGCATGCGGGGCGTGTACTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTg                      >  2:551378/1‑150 (MQ=255)
                                                                                                                         aTGNTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGt                    >  2:700094/1‑150 (MQ=255)
                                                                                                                                 aTGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGCCTAATCGTTGACTTAAATGCTCGGGAATCGCATACTCCCACTTCTCCTGAAACTCCCGTGGTACCCAATa            <  1:1051381/150‑1 (MQ=255)
                                                                                                                                  tGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGCCTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAg           >  2:646207/1‑150 (MQ=255)
                                                                                                                                   gaggggCGTGAGCACACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGc          >  1:721921/3‑150 (MQ=255)
                                                                                                                                     ggggCGTGAGCACACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCa        >  1:921479/1‑150 (MQ=255)
                                                                                                                                      gggCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCAt       <  1:700094/150‑1 (MQ=255)
                                                                                                                                           tgtgCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCATGCgac  <  1:667130/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAGTAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCATGCGAC  >  NC_002947/977735‑978023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑