Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,031,922	T→A	11.5%	L5M (TTG→ATG) ‡	PP_0889 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,031,922	0	T	A	11.5%	110.6 / 6.2	44	L5M (TTG→ATG) ‡	PP_0889	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/17);  new base A (3/2);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

TCTCGGCCACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCGACT  >  NC_002947/1031779‑1032066                                                                                                                                                |                                                                                                                                                 tctcGGCCACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGACCGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCcg                                                                                                                                            >  2:1050745/1‑150 (MQ=255)    cGGCCACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCAAGCCcgcgc                                                                                                                                         <  1:127656/150‑2 (MQ=255)     ggCCACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGaa                                                                                                                                        >  2:863563/1‑150 (MQ=255)        cACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAggg                                                                                                                                     >  2:629351/1‑150 (MQ=255)          ccccACGGGCGCTGGGCACGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATCATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCt                                                                                                                                   <  2:811207/147‑1 (MQ=255)                gggCGCTGGGCATGCACCGACGGACGCTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAg                                                                                                                             <  1:32402/150‑1 (MQ=255)                     cTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCNGTc                                                                                                                        <  2:698671/150‑1 (MQ=255)                     cTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTc                                                                                                                        >  2:670425/1‑150 (MQ=255)                           aTGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGCGGATGTGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCCATc                                                                                                                  >  1:11177/1‑150 (MQ=255)                              cACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAg                                                                                                               <  2:937467/150‑1 (MQ=255)                                           tGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGa                                                                                                  >  1:716533/1‑150 (MQ=255)                                                cgcAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCCATCAAGACGAGACCGATGAAGGtg                                                                                             >  2:341003/1‑149 (MQ=255)                                                         cAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGTGCTTGCCCGCGAAGGGCTGCAAAGCAg                                                                                                                          <  2:776827/112‑1 (MQ=255)                                                         cAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGTGCTTGCCCGCGAAGGGCTGCAAAGCAg                                                                                                                          >  1:776827/1‑112 (MQ=255)                                                             aGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGAGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATcc                                                                                <  1:629351/150‑1 (MQ=255)                                                                  ccGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTgcg                                                                           >  1:572378/1‑150 (MQ=255)                                                                                        tCGAGGTTGTTGGTCTTTTCGCGGGGGGATCCGCTCCTGCAACTTTGAGCGCGGGGGTGCTCGCGAAGGGCTGCTAAGCAGTGCCAATCAAGGCGAGCTCGATGAAGGTACTGTGGGTCATCCTGGCGCTGGTTGCAGGTAGCGCCAAAg                                                     <  2:396849/150‑1 (MQ=255)                                                                                             gatgTTGGCCTGTCCGCGGGGGAATCCGCTCCTGCAACTGTGGGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTGCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCAGACTTGCGCTGGTTGCAGGTAGCGCCAAAGCGGaa                                                <  2:537694/150‑1 (MQ=255)                                                                                               tgTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGa                                              >  1:765004/1‑150 (MQ=255)                                                                                                  tGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTg                                           <  1:863563/150‑1 (MQ=255)                                                                                                   ggCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTgg                                          <  1:967867/150‑1 (MQ=255)                                                                                                    gCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAGGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAACGCTGAAGACTGGc                                         >  2:101627/1‑150 (MQ=255)                                                                                                                  gAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTgg                           <  1:12265/150‑1 (MQ=255)                                                                                                                     tCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGGAGGGAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAg                        >  2:18814/1‑150 (MQ=255)                                                                                                                     tCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAg                        >  2:713347/1‑150 (MQ=255)                                                                                                                        ccTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAAc                     <  1:330069/149‑1 (MQ=255)                                                                                                                         cTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACg                    >  1:510452/1‑150 (MQ=255)                                                                                                                           ccTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAc                  <  1:670425/150‑1 (MQ=255)                                                                                                                            cTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAcc                 >  2:279177/1‑150 (MQ=255)                                                                                                                            cTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAcc                 <  1:279177/150‑1 (MQ=255)                                                                                                                            cTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAcc                 >  2:855006/1‑150 (MQ=255)                                                                                                                             tGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGCCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAccc                >  1:912524/1‑150 (MQ=255)                                                                                                                              gCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAcc                 >  1:551251/1‑148 (MQ=255)                                                                                                                              gCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAcc                 <  2:551251/148‑1 (MQ=255)                                                                                                                              gCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCt               >  2:411011/1‑150 (MQ=255)                                                                                                                               cAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTg              >  2:154906/1‑150 (MQ=255)                                                                                                                                    cccttcgcgGGCAAGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCAcc         >  1:835218/6‑150 (MQ=255)                                                                                                                                       ttcgcgGGCAAGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGc             >  1:313996/3‑143 (MQ=255)                                                                                                                                       ttcgcgGGCAAGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGc             <  2:313996/141‑1 (MQ=255)                                                                                                                                       ttcgcgGGCAAGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCTACACTc      >  1:439645/3‑150 (MQ=255)                                                                                                                                       aGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTc      <  1:411011/150‑1 (MQ=255)                                                                                                                                        gcgcGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCg     >  1:12456/1‑150 (MQ=255)                                                                                                                                           cgGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCGACt  <  1:44003/150‑1 (MQ=255)                                                                                                                                           cgGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCGACt  <  1:634753/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                 TCTCGGCCACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCGACT  >  NC_002947/1031779‑1032066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑