Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,130,198 A→T 19.1% intergenic (‑247/+57) gcvH‑I ← / ← PP_0990 glycine cleavage system protein H/DoxX family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,130,1980AT19.1% 105.2 / 19.8 47intergenic (‑247/+57)gcvH‑I/PP_0990glycine cleavage system protein H/DoxX family protein
Reads supporting (aligned to +/- strand):  ref base A (20/18);  new base T (5/4);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

CAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGG  >  NC_002947/1130057‑1130346
                                                                                                                                             |                                                                                                                                                    
cAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGa                                                                                                                                              >  1:579959/1‑150 (MQ=255)
 aaTAGATAACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGaa                                                                                                                                             >  2:103496/1‑150 (MQ=255)
   tAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGaaaa                                                                                                                                           <  2:1108535/150‑1 (MQ=255)
            tAAATTTAGGGAGAAGACATATTTATAGTGTGGGATGATATCGTAACAGATTCCTCTGAGAGGAAAAGGGTTGGAGCATCAAAGCCTTGTGCACCGACGGCGAAGAGGCGGTGTATCGATTTCGCTACGACGTAGCGAAAACGATACAtg                                                                                                                                  >  2:321175/1‑150 (MQ=255)
                          aGCCGTATTTATTGTGTGGAATTATATCTTTACAGGTGCCTCTGAGAGGAAAAGGGTTTCAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTg                                                                                                                    <  1:241076/150‑1 (MQ=255)
                              gTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCCGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                           <  1:77756/123‑1 (MQ=255)
                              gTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCCGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                           >  2:77756/1‑123 (MQ=255)
                               tatttattGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGc                                                                                                                            <  1:1060405/137‑1 (MQ=255)
                               tatttattGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGc                                                                                                                            >  2:1060405/1‑137 (MQ=255)
                                           ggAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGcc                                                                                                   <  2:834456/150‑1 (MQ=255)
                                             aaTGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCtt                                                                                                 >  1:549524/1‑150 (MQ=255)
                                                gATAACGTTACAGGTGCCGCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACAGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGAGAAAACGATACATGTGGTGCCTGTACTGGCGAGGTGCTAATGGCCTTCgg                                                                                              <  2:263572/150‑1 (MQ=255)
                                                       ttACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                           >  2:5692/1‑98 (MQ=255)
                                                       ttACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                           >  2:247060/1‑98 (MQ=255)
                                                       ttACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                           <  1:247060/98‑1 (MQ=255)
                                                       ttACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                           <  1:5692/98‑1 (MQ=255)
                                                               gCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTcag                                                                               >  2:628969/1‑150 (MQ=255)
                                                                          gAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCa                                                                    >  2:736504/1‑150 (MQ=255)
                                                                            aaaGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGc                                                                  <  2:918407/150‑1 (MQ=255)
                                                                                  tttGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAAc                                                            >  2:588600/1‑150 (MQ=255)
                                                                                       gCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGcc                                                       <  2:549524/150‑1 (MQ=255)
                                                                                          tCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGgc                                                    >  2:1063572/1‑150 (MQ=255)
                                                                                             aaCCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGcc                                                 >  1:999980/1‑150 (MQ=255)
                                                                                               cccTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTc                                               <  1:946979/150‑1 (MQ=255)
                                                                                                ccTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGAc                                                                                                                      <  1:435082/78‑1 (MQ=255)
                                                                                                ccTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGAc                                                                                                                      >  2:435082/1‑78 (MQ=255)
                                                                                                ccTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCg                                              >  2:223800/1‑150 (MQ=255)
                                                                                                 tttGTGCACCGAGGGCGAGAAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCgg                                             <  1:56787/149‑1 (MQ=255)
                                                                                                  ttGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCCGAAGATGATCCAGCGAAAACGCGCCGGCGCCTCggg                                            >  1:113548/1‑150 (MQ=255)
                                                                                                   tgtgCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGc                                           <  2:579959/150‑1 (MQ=255)
                                                                                                      gCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGATTGGTCAGTTGCCAATGACGATCCCTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAAc                                        <  1:981490/150‑1 (MQ=255)
                                                                                                        aCCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCtt                                                                                                 >  1:593526/1‑91 (MQ=255)
                                                                                                        aCCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCtt                                                                                                 <  2:593526/91‑1 (MQ=255)
                                                                                                        aCCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATAAATGTGGTGACTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCCCTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTGGGGGAACCa                                      >  2:36648/1‑150 (MQ=255)
                                                                                                         ccGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAg                                     <  2:447711/150‑1 (MQ=255)
                                                                                                                       gCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGcc                       <  2:999980/150‑1 (MQ=255)
                                                                                                                         tgtgTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCt                     <  1:628969/150‑1 (MQ=255)
                                                                                                                             tATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCAAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGccc                 >  2:662018/1‑150 (MQ=255)
                                                                                                                              aTCGTTTTCGCTACGACGTAGCGTAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtc                                                                                         >  2:554148/1‑77 (MQ=255)
                                                                                                                              aTCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtc                                                                                         <  1:554148/77‑1 (MQ=255)
                                                                                                                              aTCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCca                >  2:754565/1‑150 (MQ=255)
                                                                                                                                 gTTTTCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCGCTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGagca                                <  1:980150/131‑1 (MQ=255)
                                                                                                                                 gTTTTCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCGCTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGagca                                >  2:980150/1‑131 (MQ=255)
                                                                                                                                  ttttCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCACGCACCGCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcaggtc            >  1:851913/1‑150 (MQ=255)
                                                                                                                                         tACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAggtggg     >  2:49735/1‑150 (MQ=255)
                                                                                                                                         tACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAggtggg     >  1:32652/1‑150 (MQ=255)
                                                                                                                                         tACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAggtggg     <  1:1063572/150‑1 (MQ=255)
                                                                                                                                          acgacgTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAggtgggt    <  1:698365/150‑1 (MQ=255)
                                                                                                                                            gacgTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGgtgggtgg  >  2:638523/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
CAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGG  >  NC_002947/1130057‑1130346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑