Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,275,090 G→C 7.8% intergenic (‑281/+31) PP_5462 ← / ← PP_5463 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,275,0900GC 7.8% 154.3 / 4.5 51intergenic (‑281/+31)PP_5462/PP_5463hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base C (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TCCAGTGCGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGT  >  NC_002947/1274941‑1275235
                                                                                                                                                     |                                                                                                                                                 
tCCAGTGCGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCccgccg                                                                                                                                                   >  1:1090134/1‑150 (MQ=255)
   aGTGCGAATGGGCATTCTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCTGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATg                                                                                                                                                <  2:568874/150‑1 (MQ=255)
        gAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgg                                                                                                                                           >  1:148875/1‑150 (MQ=255)
        gAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgg                                                                                                                                           <  2:44802/150‑1 (MQ=255)
         aaTGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcggg                                                                                                                                          <  1:129819/150‑1 (MQ=255)
          aTGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgggc                                                                                                                                         >  1:778720/1‑150 (MQ=255)
            gggCAGTTTGGTCTATCCGGCGGAGAGTGAATGGGGAAGCCTGCTGCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCCATCGGCGGGCtt                                                                                                                                       <  2:141546/150‑1 (MQ=255)
                aTTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgg                                                                                                                                   >  2:622802/1‑150 (MQ=255)
                 ttttGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGCGCATAGCCCGCCGATGGGCGGGCTTCAggg                                                                                                                                  >  1:550195/1‑150 (MQ=255)
                   ttGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGCGGGCAAGCATGATGGGGGTGACGTTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAggggg                                                                                                                                <  1:411779/150‑1 (MQ=255)
                       tCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGc                                                                                                                            <  2:1090134/150‑1 (MQ=255)
                         tATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCCATCGGCGGGCtt                                                                                                                                       >  1:958349/1‑137 (MQ=255)
                         tATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCCATCGGCGGGCtt                                                                                                                                       <  2:958349/137‑1 (MQ=255)
                          aTCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgggg                                                                                                                                 <  2:738036/142‑1 (MQ=255)
                          aTCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgggg                                                                                                                                 >  1:738036/1‑142 (MQ=255)
                              ggcggAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAggg                                                                                                                     <  2:440781/150‑1 (MQ=255)
                                  gagaGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTAt                                                                                                                 <  2:779999/150‑1 (MQ=255)
                                      gTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGta                                                                                                             <  2:941795/150‑1 (MQ=255)
                                       ggAATGTCAAAGCCTGCTTCCCGTTTACGGCGGCTCCAATAAGGTGGGGGGGAGCGGTAATGATGGGGGGGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGtac                                                                                                            <  1:416716/149‑1 (MQ=255)
                                               gAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCg                                                                                                    >  2:423280/1‑150 (MQ=255)
                                                 atcaggCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGGGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCt                                                                                                  >  2:230655/6‑150 (MQ=255)
                                                   ccTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTtc                                                                                                <  2:1040067/150‑1 (MQ=255)
                                                     tGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTtctc                                                                                              <  1:84645/150‑1 (MQ=255)
                                                                       gCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATg                                                                                                                                                <  1:905078/82‑1 (MQ=255)
                                                                       gCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATg                                                                                                                                                >  2:905078/1‑82 (MQ=255)
                                                                       gCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGa                                                                            >  2:528238/1‑150 (MQ=255)
                                                                         tCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTa                                                                               >  1:548749/1‑145 (MQ=255)
                                                                         tCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTa                                                                               <  2:548749/145‑1 (MQ=255)
                                                                           cAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAg                                                                                  <  1:997591/140‑1 (MQ=255)
                                                                           cAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAg                                                                                  >  2:997591/1‑140 (MQ=255)
                                                                            aaTAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgggg                                                                                                                                 <  2:684215/92‑1 (MQ=255)
                                                                            aaTAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgggg                                                                                                                                 >  1:684215/1‑92 (MQ=255)
                                                                            aaTAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGt                                                                                >  1:801471/1‑141 (MQ=255)
                                                                            aaTAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGt                                                                                <  2:801471/141‑1 (MQ=255)
                                                                            aaTAAGGTGCGGGAGAGCGGAAATGAAGGGGGAGATGCTGGCGAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCAc                                                                       >  2:680670/1‑150 (MQ=255)
                                                                                 ggTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCAcccgcc                                                                  >  2:1124645/1‑150 (MQ=255)
                                                                                       ggAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTACACGCAAATGTAGGAAGCACCCGCCCGCAct                                                            >  2:257776/1‑150 (MQ=255)
                                                                                        gagaGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCTACCGAAAGGTAGGAAGCACCCGCCCGCActc                                                           >  2:722042/1‑150 (MQ=255)
                                                                                              ggTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCtcatc                                                     >  1:64298/1‑150 (MQ=255)
                                                                                                              aTGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCa                                     <  1:1124645/150‑1 (MQ=255)
                                                                                                                    gtAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAgg                                   >  1:573050/1‑146 (MQ=255)
                                                                                                                    gtAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAgg                                   <  2:573050/146‑1 (MQ=255)
                                                                                                                    gtAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGAc                               >  2:105382/1‑150 (MQ=255)
                                                                                                                         ggTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCt                          <  1:622802/150‑1 (MQ=255)
                                                                                                                         ggTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCt                          <  2:778720/150‑1 (MQ=255)
                                                                                                                           tGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCNttt                        <  2:695091/150‑1 (MQ=255)
                                                                                                                                    aaTGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCGCTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGtt               <  1:528238/150‑1 (MQ=255)
                                                                                                                                    aaTGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGtt               <  1:680670/150‑1 (MQ=255)
                                                                                                                                     ccccctgaaGCCCGCCCATCGGCGGGCTTTAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTg              >  2:725325/8‑150 (MQ=255)
                                                                                                                                         gCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATc          >  2:1039880/1‑150 (MQ=255)
                                                                                                                                               cccGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGtac                                                                                                            >  2:1093774/1‑46 (MQ=255)
                                                                                                                                               cccGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGtac                                                                                                            <  1:1093774/46‑1 (MQ=255)
                                                                                                                                                 cgccgATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGt  >  2:1108953/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TCCAGTGCGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGT  >  NC_002947/1274941‑1275235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑