| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,457,925 | C→A | 13.6% | H93N (CAC→AAC) | PP_1276 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,457,925 | 0 | C | A | 13.6% | 88.2 / 4.3 | 35 | H93N (CAC→AAC) | PP_1276 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (15/15); new base A (2/3); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TACGGGAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCC > NC_002947/1457781‑1458069 | tACGGGAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCCGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAg > 2:607082/1‑150 (MQ=255) gggAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAgcgc < 1:370994/150‑1 (MQ=255) cGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGGTCACAAGCGCGGCACAAc > 2:626340/1‑150 (MQ=255) tCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATggc > 2:750424/1‑150 (MQ=255) gTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGATCCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGaa < 2:364128/150‑1 (MQ=255) gTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCgg < 1:222183/150‑1 (MQ=255) ccGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAAc > 1:528243/1‑150 (MQ=255) cGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACt < 1:750424/150‑1 (MQ=255) cGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGa < 1:378872/150‑1 (MQ=255) ggACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGAc > 2:817894/1‑150 (MQ=255) gTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGcc < 1:817894/150‑1 (MQ=255) cGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCg > 1:567934/1‑125 (MQ=255) cGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCg < 2:567934/125‑1 (MQ=255) gATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCt > 1:776130/1‑150 (MQ=255) tGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAAt < 2:776130/150‑1 (MQ=255) tGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcc > 2:86241/1‑71 (MQ=255) tGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcc < 1:86241/72‑2 (MQ=255) gggaCTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATc > 2:775650/4‑150 (MQ=255) aggaggGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAAt > 2:945150/1‑150 (MQ=255) gggATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACaa < 2:25047/114‑1 (MQ=255) gggATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACaa > 1:25047/1‑114 (MQ=255) gATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGCATTTGAAggg > 2:931026/1‑144 (MQ=255) gATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAggg < 1:931026/144‑1 (MQ=255) gATGTCGTCATCGGGCATGAAGCGAACCGGACGCTTGTTCACAAGCGCGGCACAACCTACGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGgat < 1:607082/150‑1 (MQ=255) gtcATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTg > 1:657070/1‑150 (MQ=255) cATCGGGGGTGAGGCGAACCGAGCACATATTAACAAGAGCGGTACAACCTATGGCGGCTATGCGAAATCCGGGCATATCCGGAAACTCGAAATGCCTGTTACGGGTATTATTGCTGAGGTCTTCGAATTTGAAGGGGAGGATGATTTGCa < 1:1040973/150‑1 (MQ=255) aTCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATc < 2:563859/78‑1 (MQ=255) aTCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATc > 1:563859/1‑78 (MQ=255) ggCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAg > 2:928388/1‑122 (MQ=255) ggCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAg < 1:928388/122‑1 (MQ=255) ggCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAg < 1:1031605/150‑1 (MQ=255) aaCCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGggtg > 1:913794/1‑150 (MQ=255) cgcgcTTGTGAACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAggg < 2:654314/91‑1 (MQ=255) cgcgcTTGTGAACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAggg > 1:654314/2‑92 (MQ=255) ttGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGcc < 2:984322/150‑1 (MQ=255) | TACGGGAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCC > NC_002947/1457781‑1458069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |