Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,750,775 C→G 9.5% A31G (GCC→GGC)  PP_1551 → replication protein O

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,750,7750CG 9.5% 125.2 / 5.1 43A31G (GCC→GGC) PP_1551replication protein O
Reads supporting (aligned to +/- strand):  ref base C (21/17);  new base G (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

CGAAAAAAAACCGCCTGGCAGGGCGGCTTTCTCTACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAGGCGGTTTCCAGCCTGCTGCGT  >  NC_002947/1750627‑1750920
                                                                                                                                                    |                                                                                                                                                 
cGAAAAAAAACCGCCTGGCAGGGCGGCTTTCTCTACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTcccggcc                                                                                                                                                  >  2:838306/1‑150 (MQ=255)
 gAAAAAAAACCGCCTGGCAGGGCGGCTTTCTCTACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCCCTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTcccggccc                                                                                                                                                 >  1:687970/1‑150 (MQ=255)
 gAAAAAAAACCGCCTGGCAGGGCGGCTTTCTCTACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTcccggccc                                                                                                                                                 >  1:147395/1‑150 (MQ=255)
        aaCCGCCTGGCAGGGCGGCTTTCTCTACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGGCCGGGAGAt                                                                                                                                          <  2:330937/150‑1 (MQ=255)
                  cAGGGCGGCTTTCTCTACAGCATTAGAACGGGTTAAAGCCTGCCAAACATCTTCCCACTTGACAAGTCCAGAGCGATCTCCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATtgtg                                                                                                                                <  1:624869/150‑1 (MQ=255)
                   aGGGCGGCTTTCTCTACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGgaga                                                                                                                                           <  2:823985/138‑1 (MQ=255)
                   aGGGCGGCTTTCTCTACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGgaga                                                                                                                                           >  1:823985/1‑138 (MQ=255)
                            ttCTCTACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTgg                                                                                                                      >  1:693146/1‑150 (MQ=255)
                              ctctACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGcc                                                                                                                    <  1:838306/150‑1 (MQ=255)
                                 tACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAg                                                                                                                 <  1:848596/150‑1 (MQ=255)
                                 tACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAg                                                                                                                 <  2:438389/150‑1 (MQ=255)
                                       aTTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGccacc                                                                                                           <  2:693146/150‑1 (MQ=255)
                                         tAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCAt                                                                                                         <  2:1009178/150‑1 (MQ=255)
                                                       gCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCCTCAACTTCGGTGCgg                                                                                           >  1:507442/1‑150 (MQ=255)
                                                           gACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGgcgc                                                                                       <  2:687970/150‑1 (MQ=255)
                                                                      tCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATcc                                                                            >  2:942190/1‑150 (MQ=255)
                                                                           cTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCt                                                                       >  2:426331/1‑150 (MQ=255)
                                                                                cAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGa                                                                  >  2:686194/1‑150 (MQ=255)
                                                                                 aaGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGAc                                                                 >  1:328314/1‑150 (MQ=255)
                                                                                      cAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCg                                                                          <  1:373085/136‑1 (MQ=255)
                                                                                      cAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCg                                                                          >  2:373085/1‑136 (MQ=255)
                                                                                       aGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATcgcg                                                           >  1:277881/1‑150 (MQ=255)
                                                                                       aGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCAGCTCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTCAGGTGCGGGCGCTCAACGCATCCCGGCTCACGACCTcaac                                                           >  2:283796/1‑147 (MQ=255)
                                                                                         ggggTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGaa                                                         >  2:262856/1‑150 (MQ=255)
                                                                                          gggTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTACACTCATCCCGGCTACCGACATCGCGaaa                                                        <  1:426331/150‑1 (MQ=255)
                                                                                            gTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTCCGTTTCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCCGCTACCGACATCGCGAAAGc                                                      >  1:620268/1‑150 (MQ=255)
                                                                                                aCCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATc                                                  >  1:802110/1‑150 (MQ=255)
                                                                                                  ccGGATGGACACCCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAAATTGGGCTGTACGTGGCCAAGGCCACCATCAACATCGGCGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCCAAAACCATCCa                                                >  2:361471/1‑150 (MQ=255)
                                                                                                  ccGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCa                                                >  1:387803/1‑150 (MQ=255)
                                                                                                    ggATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCg                                                                                                 <  1:424598/99‑1 (MQ=255)
                                                                                                    ggATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCg                                                                                                 >  2:424598/1‑99 (MQ=255)
                                                                                                          aCAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCAcc                                        <  1:686194/150‑1 (MQ=255)
                                                                                                              ccAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGa                                    <  2:147395/150‑1 (MQ=255)
                                                                                                              ccAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGa                                    <  1:262856/150‑1 (MQ=255)
                                                                                                                aGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCAt                                                   >  2:423144/1‑133 (MQ=255)
                                                                                                                aGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCAt                                                   <  1:423144/133‑1 (MQ=255)
                                                                                                                aGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGaca                                  >  2:493805/1‑150 (MQ=255)
                                                                                                                        gatggCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGCAACCGTGTcc                          >  1:597861/1‑150 (MQ=255)
                                                                                                                           ggCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAg                       <  1:253501/150‑1 (MQ=255)
                                                                                                                               tgctgGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCCAGTTATGATGGCATCCCGGCTACGGACATCGCGAAAAGCCTCAAATCTTACCTTTACAGCGTGTCCAAGGCgg                   >  1:20326/1‑150 (MQ=255)
                                                                                                                                   ggCTATCGATCTCCTGGAACGAGAGATGAAGATTGTGCTTTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAGGCGGTTTc               <  1:191546/150‑1 (MQ=255)
                                                                                                                                       aTCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAGGCGGTTTCCAGc           <  2:277881/150‑1 (MQ=255)
                                                                                                                                        ttcaTCTCCCGGGCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAGGCGGTTTCCAGcc          >  2:803206/4‑150 (MQ=255)
                                                                                                                                         tcaTCTCCCGGGCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAgg                      <  2:421785/135‑1 (MQ=255)
                                                                                                                                         tcaTCTCCCGGGCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAgg                      >  1:421785/3‑137 (MQ=255)
                                                                                                                                              tcCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAGGCGGTTTCCAGCCtgctgc    >  1:241298/1‑150 (MQ=255)
                                                                                                                                               cccggcccggGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGTGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCAACCACGCTCACCCTGACACCGTGTCCAAGGCGGTTTCCAGCCTGCTGCg   >  1:1098627/1‑150 (MQ=255)
                                                                                                                                                ctggcccggGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAGGCGGTTTCCAGCCTGCTGCGt  >  2:179429/3‑150 (MQ=255)
                                                                                                                                                ccggcccggGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAGGCGGttt                <  1:815794/136‑1 (MQ=255)
                                                                                                                                                ccggcccggGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAGGCGGttt                >  2:815794/1‑136 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGAAAAAAAACCGCCTGGCAGGGCGGCTTTCTCTACAGCATTAGAACGGGTTAAAGCATGACAAACATCGTCCCACTTGACAAGTCCAGGGGGTTCACCCGGATGGACAACCAGCTCATGGATGGCCTGCTGGCTATCGATCTCCCGGCCCGGGAGATGAAGATTGTGCTGTACGTGGCCAAGGCCACCATCAACTTCGGTGCGGGCGCTCAACGCATCCCGGCTACCGACATCGCGAAAGCCATCCACGCTCACCCTGACACCGTGTCCAAGGCGGTTTCCAGCCTGCTGCGT  >  NC_002947/1750627‑1750920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑