| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,827,748 | T→C | 10.5% | L187P (CTG→CCG) | recA → | recombinase RecA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,827,748 | 0 | T | C | 10.5% | 93.3 / 3.5 | 38 | L187P (CTG→CCG) | recA | recombinase RecA |
| Reads supporting (aligned to +/- strand): ref base T (16/18); new base C (2/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01 | |||||||||||
GACGTGATCATTGTCGACTCCGTTGCCGCGCTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTATGCTTCGGTCCGTCTGGACATCCGCCGTACCGGCGCGGTCAAGGAAGGCGACGAAG > NC_002947/1827600‑1827894 | gACGTGATCATTGTCGACTCCGTTGCCGCGCTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTg > 2:604234/1‑150 (MQ=255) aTCATTGTCGACTCCGTTGCCGGGCTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATc < 2:139320/150‑1 (MQ=255) gTCGACTCCGTTGCCGCGCTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTtcatc < 2:86435/150‑1 (MQ=255) gACTCCGTTGCCGCGCTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAAc < 1:604234/150‑1 (MQ=255) tCCGTTGCCGCGCTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAg > 1:305381/1‑150 (MQ=255) cgcgCTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTAt > 2:1005442/1‑150 (MQ=255) gcTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCATGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGaa < 1:660952/150‑1 (MQ=255) gcTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCATGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGaa < 1:660933/150‑1 (MQ=255) aCCGAAGGCCGAGATCGAAGGCGAGATGGGTGGCATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCgg > 2:659597/1‑150 (MQ=255) cGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGgtg > 2:420783/1‑150 (MQ=255) gAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGGAACATCAAAAACGCGAACTGCCTGGTCATCTTCATCAACCAGCTCCGTATGAAGATCGgtgt > 1:234365/1‑150 (MQ=255) aGGCCGAGATCGAAGGGGAGATGGGTGACATGCATGTGGGACTACAGTCCCGCCTGATGTCCCAGGCACTGGCTAACTTCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGa < 1:247764/150‑1 (MQ=255) agaTCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCg > 1:940458/1‑150 (MQ=255) gAAGGCGAGTTGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTAGGCAGc < 2:305381/150‑1 (MQ=255) gAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGc > 1:541243/1‑150 (MQ=255) ggCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCg > 2:90958/1‑150 (MQ=255) ggTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACt > 1:866371/1‑150 (MQ=255) gTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTa < 1:420783/150‑1 (MQ=255) cTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGACTGGTCATCTTCATCAACCAGAACCGACTGAAGATTAGCGTGATGTTCGGCAGCCAGGAAACCGCTACCGGTGGAAACGCCCTg > 2:346497/1‑150 (MQ=255) gCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTc < 1:122022/150‑1 (MQ=255) ccGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCa < 1:512668/78‑1 (MQ=255) ccGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCa > 2:512668/1‑78 (MQ=255) ccGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTa < 2:866371/150‑1 (MQ=255) gCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGAt > 2:549073/1‑79 (MQ=255) gCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGAt < 1:549073/79‑1 (MQ=255) gTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTATGCTTCGGt < 2:940458/150‑1 (MQ=255) cAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCCGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCa > 1:896192/1‑97 (MQ=255) cAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCCGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCa > 1:900970/1‑97 (MQ=255) cAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCCGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCa < 2:896192/97‑1 (MQ=255) cAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCCGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCa < 2:900970/97‑1 (MQ=255) tGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTa < 2:508721/105‑1 (MQ=255) tGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTa > 1:508721/1‑105 (MQ=255) gTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTa > 2:219032/1‑127 (MQ=255) gTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTa < 1:219032/127‑1 (MQ=255) aCCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTATGCTTCGGTCCGTCTGGACATCCGCCGTAcc > 2:1033645/1‑150 (MQ=255) ccGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTATGCTTCGGTCCGTCTGGACATCCGCCGTACCg < 1:90958/150‑1 (MQ=255) cgcgAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTATGCTTCGGTCCGTCTGGACATCCGCCGTACCGGCGCGGTCaaggaagg < 1:1033645/150‑1 (MQ=255) gCCTGGTCATCTTCATCAACCAGATCCGTATGAGGATCGGTGTGGTGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTATGCTTCGGTCCGTCTGGACATCCGCCGTACCGTCGCGGCCAAGGGAGGCGACGAAg < 2:910825/150‑1 (MQ=255) | GACGTGATCATTGTCGACTCCGTTGCCGCGCTGGTACCGAAGGCCGAGATCGAAGGCGAGATGGGTGACATGCATGTGGGCCTGCAGGCCCGCCTGATGTCCCAGGCACTGCGTAAGATCACCGGTAACATCAAGAACGCGAACTGCCTGGTCATCTTCATCAACCAGATCCGTATGAAGATCGGTGTGATGTTCGGCAGCCCGGAAACCACTACCGGTGGTAACGCCCTGAAGTTCTATGCTTCGGTCCGTCTGGACATCCGCCGTACCGGCGCGGTCAAGGAAGGCGACGAAG > NC_002947/1827600‑1827894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |