Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,850,958 T→A 100% M1K (ATG→AAG) † relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,850,9580TA100.0% 168.7 / NA 50M1K (ATG→AAG) †relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (27/23);  total (27/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGACGGCGCATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAA  >  NC_002947/1850817‑1851104
                                                                                                                                             |                                                                                                                                                  
aGACGGCGCATGTCGAGGCCATGGCGTTATTCGAAGCGGGCGAGCGTACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAgg                                                                                                                                            >  2:770685/1‑150 (MQ=255)
 gACGGCGCATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCAAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGt                                                                                                                                           <  1:798908/150‑1 (MQ=255)
   cGGCGCATGTCGAGGCCATGGCGTCATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTCAAACAACGAACGTACAGGTga                                                                                                                                         <  2:565275/150‑1 (MQ=255)
     gcgcATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTgaga                                                                                                                                       <  1:165537/150‑1 (MQ=255)
       gcATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGATACGGTTACCAATAAAGGGCGGCGGCTCGGTGATTGTCAGCACTCTCGTATGGTGTGCTGCATGGAAAGGTAAAAACATGTAGGTACAGGTGagagg                                                                                                                                     >  1:607415/1‑149 (MQ=255)
        cATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCAAGAAGGAGCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTg                                                                                                                                    <  2:651261/150‑1 (MQ=255)
               aGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGGATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGc                                                                                                                             <  2:56067/150‑1 (MQ=255)
                 gCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCg                                                                                                                           <  2:723807/150‑1 (MQ=255)
                  ccATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCgg                                                                                                                          <  2:1099523/150‑1 (MQ=255)
                   cATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGt                                                                                                                         <  2:757274/150‑1 (MQ=255)
                   cATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGt                                                                                                                         <  2:1013566/150‑1 (MQ=255)
                    aTGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTc                                                                                                                        >  2:116053/1‑150 (MQ=255)
                       gCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAac                                                                                                                     >  2:96893/1‑150 (MQ=255)
                             ttCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGAc                                                                                                               >  2:889495/1‑150 (MQ=255)
                                             tGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCg                                                                                               >  2:394179/1‑150 (MQ=255)
                                                   gCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCAt                                                                                         >  1:797234/1‑150 (MQ=255)
                                                     cccTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATgg                                                                                       >  1:389144/1‑150 (MQ=255)
                                                                gTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgt                                                                            >  2:6852/1‑150 (MQ=255)
                                                                gTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgt                                                                            >  2:512439/1‑150 (MQ=255)
                                                                gTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgt                                                                            <  1:889495/150‑1 (MQ=255)
                                                                 ttGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgtg                                                                           <  1:580625/150‑1 (MQ=255)
                                                                   gCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGt                                                                         <  1:770685/150‑1 (MQ=255)
                                                                   gCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGt                                                                         <  1:116053/150‑1 (MQ=255)
                                                                     cACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAac                                                                                                                     <  1:696130/104‑1 (MQ=255)
                                                                     cACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAac                                                                                                                     >  2:696130/1‑104 (MQ=255)
                                                                              gcggcCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGt                                                                   <  1:955628/145‑1 (MQ=255)
                                                                              gcggcCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGt                                                                   >  2:955628/1‑145 (MQ=255)
                                                                              gcggcCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGAtt                                                              >  1:811367/1‑150 (MQ=255)
                                                                                                ccAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCgg                                                           >  1:992944/1‑135 (MQ=255)
                                                                                                ccAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCgg                                                           <  2:992944/135‑1 (MQ=255)
                                                                                                       acTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGaa                                     >  1:692297/1‑150 (MQ=255)
                                                                                                         tCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGa                                      <  1:87554/147‑1 (MQ=255)
                                                                                                         tCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGa                                      >  2:87554/1‑147 (MQ=255)
                                                                                                         tCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAaag                                   >  1:8985/1‑150 (MQ=255)
                                                                                                         tCGTATGGTGTGCTACAGTTAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAaag                                   >  1:879254/1‑150 (MQ=255)
                                                                                                           gTATGGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAaagaa                                 <  1:6852/150‑1 (MQ=255)
                                                                                                                       gcATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGttt                     <  2:797234/150‑1 (MQ=255)
                                                                                                                        cATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTg                    >  2:137231/1‑150 (MQ=255)
                                                                                                                            gAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGAACGCAGTAGCAATCTCGAAGCATGGGTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGctct                >  1:412010/1‑150 (MQ=255)
                                                                                                                               aGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGa             <  1:512439/150‑1 (MQ=255)
                                                                                                                                 gTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAgg           >  2:887568/1‑150 (MQ=255)
                                                                                                                                  tAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGAtt                                                              <  2:73221/98‑1 (MQ=255)
                                                                                                                                  tAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGAtt                                                              >  1:73221/1‑98 (MQ=255)
                                                                                                                                  tAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGt          >  2:194465/1‑150 (MQ=255)
                                                                                                                                  tAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCCAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGCAAGAAGCCTGCGCGTTTGCTCTCGAggg          >  1:847488/1‑149 (MQ=255)
                                                                                                                                    aaaCAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAgcg                                          >  1:722763/1‑116 (MQ=255)
                                                                                                                                    aaaCAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAgcg                                          <  2:722763/116‑1 (MQ=255)
                                                                                                                                        aaaGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCaa                                                                                                    >  2:921610/1‑54 (MQ=255)
                                                                                                                                        aaaGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCaa                                                                                                    <  1:921610/54‑1 (MQ=255)
                                                                                                                                          aGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGAATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGaaaa  >  2:195549/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
AGACGGCGCATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATGGTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGAAAA  >  NC_002947/1850817‑1851104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑