Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,977,279 C→G 9.1% R300R (CGC→CGG)  PP_1770 → 3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,977,2790CG 9.1% 119.4 / 2.7 40R300R (CGC→CGG) PP_17703‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (13/23);  new base G (2/2);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACGCTGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAGGTACTACCG  >  NC_002947/1977131‑1977427
                                                                                                                                                    |                                                                                                                                                    
gcgACGCTGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCccgcc                                                                                                                                                     >  2:1040154/1‑150 (MQ=255)
  gACGCTGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCgg                                                                                                                                                   <  2:721426/150‑1 (MQ=255)
      cTGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCt                                                                                                                                               <  2:206005/150‑1 (MQ=255)
         tCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGGCGGGccagga                                                                                                                                            <  1:163537/150‑5 (MQ=255)
         tCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGGCGGGccagga                                                                                                                                            <  1:443685/150‑5 (MQ=255)
           cTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTAtgtg                                                                                                                                          <  2:249550/150‑1 (MQ=255)
              cgcACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAACCCCTGGCCCGCCGGGCCTATGTGGAc                                                                                                                                       <  1:207622/150‑1 (MQ=255)
                cACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGc                                                                                                                                     <  1:945048/150‑1 (MQ=255)
                  cGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTa                                                                                                                                   <  1:934891/150‑1 (MQ=255)
                         tACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGc                                                                                                                            >  1:778826/1‑150 (MQ=255)
                             tttCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAAc                                                                                                                        <  1:222111/150‑1 (MQ=255)
                                 gCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATc                                                                                                                    <  2:316995/150‑1 (MQ=255)
                                 gCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATc                                                                                                                    <  1:965180/150‑1 (MQ=255)
                                                   tGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAAc                                                                                                  >  2:829242/1‑150 (MQ=255)
                                                            cgaTCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGgcc                                                                                         <  1:89724/150‑1 (MQ=255)
                                                             gaTCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGgccg                                                                                        >  1:872220/1‑150 (MQ=255)
                                                              aTCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGgccgc                                                                                       <  2:39928/150‑1 (MQ=255)
                                                              aTCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGgccgc                                                                                       >  1:43403/1‑150 (MQ=255)
                                                                cGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCt                                                                                     <  2:609601/150‑1 (MQ=255)
                                                                   tGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCGCGGGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGaa                                                                                  >  1:747817/1‑150 (MQ=255)
                                                                      aGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACgg                                                                               <  1:364077/150‑1 (MQ=255)
                                                                      aGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCCCTCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACgg                                                                               <  1:1040154/150‑1 (MQ=255)
                                                                               gCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCg                                                                      >  1:599456/1‑150 (MQ=255)
                                                                                cACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGCCTACTAACGATCTGATTTTCCAAGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCgt                                                                     >  1:852306/1‑150 (MQ=255)
                                                                                     gctgctGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTAcc                                                                >  2:754178/1‑150 (MQ=255)
                                                                                        cctgcttgtATTCACCCGCGCACCGGTTGCCCGCCAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCggg                                                             <  1:587207/144‑1 (MQ=255)
                                                                                           gggTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGa                                                          <  1:754178/150‑1 (MQ=255)
                                                                                                   tCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGa                                                  >  1:992738/1‑150 (MQ=255)
                                                                                                    cACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGTCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGAt                                                 <  2:599456/150‑1 (MQ=255)
                                                                                                       ccGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATcc                                                                       >  2:108929/1‑125 (MQ=255)
                                                                                                       ccGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATcc                                                                       <  1:108929/125‑1 (MQ=255)
                                                                                                          cgcACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCa                                           <  1:998776/150‑1 (MQ=255)
                                                                                                           gcACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGCTTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCAt                                          <  1:349017/150‑1 (MQ=255)
                                                                                                            cACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATc                                         >  1:559559/1‑150 (MQ=255)
                                                                                                                      ccGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCAt                               >  2:1117686/1‑150 (MQ=255)
                                                                                                                                   aGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATAAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTg                  >  1:219559/1‑150 (MQ=255)
                                                                                                                                    gTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTgg                 <  1:829242/150‑1 (MQ=255)
                                                                                                                                         acataggCCCGGCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGaa            >  1:449384/6‑150 (MQ=255)
                                                                                                                                          tCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAg           <  1:101787/150‑1 (MQ=255)
                                                                                                                                             tGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAGGta        <  2:219559/150‑1 (MQ=255)
                                                                                                                                             tGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGCGGAAGAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAGGta        >  1:618989/1‑150 (MQ=255)
                                                                                                                                                   gccgGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAGGTACTACCg  <  1:935546/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGACGCTGTCCTGCGCACGCTTGATACATTTCGCAGTGATCTCGACGCCCTGCGCGACGCGATCGCTGAAGGGGATGGGCACCAGCTGCTGGGTGTATTCACCCGCGCACGGGTTGCCCGCGAGCATTTCAGTAAAATCCTGGCCCGCCGGGCCTATGTGGACGCTATGAACGCCAACGATCTGATTTTCCAGGCCCAACCGGGTGGCCGCCTGAACGGGCGGATCCGTGTACCGGGCGACAAGTCGATTTCCCATCGCTCGATCATGCTCGGCTCGCTGGCCGAAGGTACTACCG  >  NC_002947/1977131‑1977427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑