Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,041,980 C→A 10.3% G207C (GGC→TGC)  PP_1816 ← alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,041,9800CA10.3% 103.5 / 3.1 39G207C (GGC→TGC) PP_1816alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (16/19);  new base A (2/2);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01

CCGCTTCTTTGTTGTTGTACTGGCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTGGCAATCTGCCCGACAATGCTGCCCACCGC  >  NC_002947/2041833‑2042127
                                                                                                                                                   |                                                                                                                                                   
ccGCTTCTTTGTTGTTGTACTGGCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAggccgg                                                                                                                                                   >  2:1101099/1‑150 (MQ=255)
 cGCTTCTTTGTTGTTGTACTGGCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAggccggc                                                                                                                                                  <  1:667609/150‑1 (MQ=255)
 cGCTTCTTTGTTGTTGTACTGGCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAggccggc                                                                                                                                                  >  1:531799/1‑150 (MQ=255)
 cGCTTCTTTGTTGTTGTACTGGCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAggccggc                                                                                                                                                  >  2:667609/1‑150 (MQ=255)
  gCTTCTTTGTTGTTGTACTGGCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAggccggcc                                                                                                                                                 >  2:649938/1‑150 (MQ=255)
                 tACTGGCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGcc                                                                                                                                  <  2:222600/150‑1 (MQ=255)
                   cTGGCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCtt                                                                                                                                <  1:291466/150‑1 (MQ=255)
                      gCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTa                                                                                                                             >  2:1120210/1‑150 (MQ=255)
                      gCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTa                                                                                                                             <  1:1020207/150‑1 (MQ=255)
                             gcgcCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATc                                                                                                                      >  2:677588/1‑150 (MQ=255)
                             gcgcCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATc                                                                                                                      <  1:649938/150‑1 (MQ=255)
                                ccgcAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACg                                                                                                                   >  1:581069/1‑150 (MQ=255)
                                     aaTCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTc                                                                                                              >  1:1086083/1‑150 (MQ=255)
                                         acaATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAg                                                                                                          >  1:165194/1‑150 (MQ=255)
                                               cGTGCCTTGTAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTATGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGc                                                                                                    <  2:56959/150‑1 (MQ=255)
                                               cGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGc                                                                                                    <  1:1101099/150‑1 (MQ=255)
                                                   ccTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACTCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCCAAGCCCAGCTCGt                                                                                                >  1:481058/1‑150 (MQ=255)
                                                        aaGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTc                                                                                           <  1:677588/150‑1 (MQ=255)
                                                        aaGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCAGCCAAAGTCCAGTGcttccttc                                                                                           <  2:1123176/150‑1 (MQ=255)
                                                              aTGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTAc                                                                                     <  2:1070724/150‑1 (MQ=255)
                                                               tGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACt                                                                                    >  2:724631/1‑150 (MQ=255)
                                                                    gTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGACCCCGCCGCCAACGATCCCGTCGGCCTTCAGGTACTGGCAc                                                                               >  1:516335/1‑150 (MQ=255)
                                                                        gCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTtct                                                                           >  1:505788/1‑150 (MQ=255)
                                                                        gCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTtcg                                                                           >  1:91279/1‑149 (MQ=255)
                                                                                 cGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCAccgc                                                                  <  1:1120210/150‑1 (MQ=255)
                                                                                    ggATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCt                                                                                                                                 >  2:609258/1‑84 (MQ=255)
                                                                                    ggATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCt                                                                                                                                 <  1:609258/84‑1 (MQ=255)
                                                                                    gaatacCGGCGCCCACCTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCATGTAGCCGATCACGCCGTCAAACCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCgg                                                               <  2:61194/148‑1 (MQ=255)
                                                                                               cccACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGAcc                                                    >  2:206964/1‑150 (MQ=255)
                                                                                                        tCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTAGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAg                                           >  1:1079067/1‑150 (MQ=255)
                                                                                                          gAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCAGGCCAGCACGTCTTCGGCCTTGTa                                                                                                                             <  2:1037218/66‑1 (MQ=255)
                                                                                                          gAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCAGGCCAGCACGTCTTCGGCCTTGTa                                                                                                                             <  2:1037214/66‑1 (MQ=255)
                                                                                                          gAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCAGGCCAGCACGTCTTCGGCCTTGTa                                                                                                                             >  1:1037218/1‑66 (MQ=255)
                                                                                                          gAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCAGGCCAGCACGTCTTCGGCCTTGTa                                                                                                                             >  1:1037214/1‑66 (MQ=255)
                                                                                                            aGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCATAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCtt                                       <  2:998955/150‑1 (MQ=255)
                                                                                                                 gcGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATc                                  <  2:174757/149‑1 (MQ=255)
                                                                                                                    tCCACGCCTTTCGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAATGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCCCCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTg                               <  1:72470/150‑1 (MQ=255)
                                                                                                                    gccACGGCTTTGGGGCATTCGCGCGTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTg                               <  2:165194/149‑1 (MQ=255)
                                                                                                                     ccACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTgg                              <  1:724631/150‑1 (MQ=255)
                                                                                                                     ccACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTgg                              <  2:722709/150‑1 (MQ=255)
                                                                                                                               gtggCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTGGCAATCTGccc                    <  1:206964/148‑1 (MQ=255)
                                                                                                                                   cATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTGGCAATCTGCCCGACa                >  1:128718/1‑150 (MQ=255)
                                                                                                                                     ttCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCCGCCTTTGATCTTGGCAATCTGCCCGACAAt              >  2:178810/1‑150 (MQ=255)
                                                                                                                                            ttCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTGGCAATCTGCCCGACAATGCTGccc       >  1:132746/1‑150 (MQ=255)
                                                                                                                                                 gccggccAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTGGCAATCTGCCCGACAATGCTGCCcaccgc  >  2:926773/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGCTTCTTTGTTGTTGTACTGGCTGATCGCGCCGCAAATCACAATGCGTGCCTTGAAGTTGATGCGCGTCAGCACGGCATCGAGGATATCGCCGCCCACGTTGTCGAAGTACACGTCCACGCCTTTGGGGCATTCGCGCTTCAGGCCGGCCAGCACGTCTTCGGCCTTGTAGTCGATCACGCCGTCAAAGCCCAGCTCGTCCTTCAGGTACTGGCACTTCTCGGCACCGCCGGCAATGCCGACCACGCGGCAGCCTTTGATCTTGGCAATCTGCCCGACAATGCTGCCCACCGC  >  NC_002947/2041833‑2042127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑