Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,139,665	T→G	11.1%	Q167H (CAA→CAC)	PP_5481 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,139,665	0	T	G	11.1%	105.4 / 6.9	45	Q167H (CAA→CAC)	PP_5481	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/20);  new base G (3/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

TTCCGCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAA  >  NC_002947/2139518‑2139814                                                                                                                                                    |                                                                                                                                                      ttCCGCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTg                                                                                                                                                     <  2:1083737/150‑1 (MQ=255)   ccGCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGc                                                                                                                                                   <  2:949908/150‑1 (MQ=255)     gCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcga                                                                                                                                                 >  1:1010808/1‑150 (MQ=255)     gCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcga                                                                                                                                                 >  1:364725/1‑150 (MQ=255)         cGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCgacgac                                                                                                                                             >  1:162043/1‑150 (MQ=255)          ggCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGacgacg                                                                                                                                            >  2:395703/1‑150 (MQ=255)                  aGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGcc                                                                                                                                    <  2:1039817/150‑1 (MQ=255)                                           aCGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTAGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACAGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGaa                                                                                                           >  2:427841/1‑150 (MQ=255)                                            agtggATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAAc                                                                                                          >  2:380819/5‑150 (MQ=255)                                                  tCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtggtg                                                                                                    <  2:803386/150‑1 (MQ=255)                                                      cTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGgaga                                                                                                >  1:962475/1‑150 (MQ=255)                                                      cTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGgaga                                                                                                >  2:998354/1‑150 (MQ=255)                                                          ttGCTGTTGTCCTCTGCGGTGCTCTACGTTGTTTGAGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTa                                                                                            <  2:456819/149‑1 (MQ=255)                                                          aTGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTa                                                                                            <  2:364725/150‑1 (MQ=255)                                                           tGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                           <  1:47356/150‑1 (MQ=255)                                                           tGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                           >  2:992849/1‑150 (MQ=255)                                                                gtggCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGTCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAg                                                                                      >  1:842686/1‑150 (MQ=255)                                                                  ggCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCTGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGa                                                                                    <  2:596131/150‑1 (MQ=255)                                                                       cTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCCGGCTGGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGccc                                                                               <  1:291080/150‑1 (MQ=255)                                                                             tGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGcacc                                                                         >  2:1053351/1‑150 (MQ=255)                                                                                tctACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcg                                                                      >  2:494706/1‑150 (MQ=255)                                                                                 ctACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcgc                                                                     <  2:910470/150‑1 (MQ=255)                                                                                  tACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCAGTGGCGTCGTCGCCAATGCCg                                                                                                                                   >  1:261310/1‑87 (MQ=38)                                                                                  tACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCAGTGGCGTCGTCGCCAATGCCg                                                                                                                                   <  2:261310/87‑1 (MQ=37)                                                                                     gttgttTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGca                                                                 >  1:914031/1‑150 (MQ=255)                                                                                              gTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccga                                                        <  1:998354/150‑1 (MQ=255)                                                                                                    ttGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgc                                                                                                                     >  2:522513/1‑83 (MQ=255)                                                                                                    ttGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgc                                                                                                                     <  1:522513/83‑1 (MQ=255)                                                                                                    ttGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccgag                                                  <  2:1010808/150‑1 (MQ=255)                                                                                                       cTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAg                                               >  1:919018/1‑150 (MQ=255)                                                                                                          gTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGaa                                            >  1:37811/1‑150 (MQ=255)                                                                                                          gTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGaa                                            >  2:252636/1‑150 (MQ=255)                                                                                                           tCAGCGGACGCGCCATGCTGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCAAGGGCGCCCAGCACACTAGCGCCAGTAGAACGTGGTGCCGCAGTAGGCAAGGTTCCACAGCTCCTCTCCTCACACCACCGAGCCGAGCAGGAAg                                           <  1:252636/150‑1 (MQ=255)                                                                                                            cAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGGAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAgg                                          <  2:547016/150‑1 (MQ=255)                                                                                                                      ggCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAg                                <  2:919018/150‑1 (MQ=255)                                                                                                                         aTGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGt                             >  1:238855/1‑150 (MQ=255)                                                                                                                                   gAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTgg                   <  1:1053351/150‑1 (MQ=255)                                                                                                                                       ccTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCgg               <  1:389623/150‑1 (MQ=255)                                                                                                                                         ttGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCTAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTg             >  2:438088/1‑150 (MQ=255)                                                                                                                                         ttGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGGAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGGTGCCACATCGTCGACAGCTGGTCGGTg             >  2:922442/1‑150 (MQ=255)                                                                                                                                          tGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGa            <  2:962475/150‑1 (MQ=255)                                                                                                                                            ggccGGCAGTGGCGTCGTCGCCAATGCCGGTCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGc                                     >  2:28854/3‑123 (MQ=38)                                                                                                                                            ggccGGCAGTGGCGTCGTCGCCAATGCCGGTCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGc                                     <  1:28854/121‑1 (MQ=38)                                                                                                                                                ggCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACt      <  1:380819/150‑1 (MQ=255)                                                                                                                                                 gCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTg     >  1:884390/1‑150 (MQ=255)                                                                                                                                                    ttGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGaa  <  1:494706/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TTCCGCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAA  >  NC_002947/2139518‑2139814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑