| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,240,419 | A→T | 9.5% | D202V (GAC→GTC) | gltX → | glutamate‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,240,419 | 0 | A | T | 9.5% | 119.0 / 3.9 | 42 | D202V (GAC→GTC) | gltX | glutamate‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base A (18/20); new base T (2/2); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
AAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGAAA > NC_002947/2240283‑2240567 | aaGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACTACCACCTGATg < 2:325181/150‑1 (MQ=255)aaGGTGCCGAGCGAAGGGATCTGCGTGGGGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATg < 1:737476/150‑1 (MQ=255) ggATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGGTGAAGAACGACGGCCTGCCGACGTGCTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCAccaccgggc > 2:487771/1‑143 (MQ=255) cTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCg > 1:929685/1‑150 (MQ=255) cGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgg < 2:147134/150‑1 (MQ=255) gCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAaga < 2:18770/150‑1 (MQ=255) gCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAaga < 1:761724/150‑1 (MQ=255) gCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAaga > 2:386444/1‑150 (MQ=255) cGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGt < 1:487771/150‑1 (MQ=255) gctgcGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCAc < 1:367300/124‑1 (MQ=255) gctgcGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCAc > 2:367300/1‑124 (MQ=255) tgcGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCAt > 2:935439/1‑150 (MQ=255) ggcgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcg < 1:386444/150‑1 (MQ=255) gcgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcgc < 2:929685/150‑1 (MQ=255) gcgATGTCGAGATCCCGTGGGACCGCATGGAAATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcgc < 1:41602/150‑1 (MQ=255) gATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGccc < 1:395187/150‑1 (MQ=255) aTGTCGAGATCCCGTGGGACCGCAGGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGAGGCTGCCATCGGCGCCCa > 2:473759/1‑150 (MQ=255) tCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGTCGaccacgttg > 2:239964/1‑92 (MQ=255) tCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGTCGaccacgttg < 1:239964/96‑5 (MQ=255) ccGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCaaa > 2:983995/1‑150 (MQ=255) gggACCGCATGGACATGCAGGTGCTGATGACGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGt < 2:336915/150‑1 (MQ=255) gaaTGGACATGCAGGTGCTGATAAAGAACGACGGACTGTCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACTTACTTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGt > 2:206710/3‑150 (MQ=255) gCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGagca < 1:935439/150‑1 (MQ=255) cTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGagcag > 1:341227/1‑150 (MQ=255) tgatgaAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTgg < 1:661941/95‑1 (MQ=255) tgatgaAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTgg > 2:661941/1‑95 (MQ=255) gaagaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCaa > 1:494668/1‑150 (MQ=255) gaagaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCaa > 1:494863/1‑150 (MQ=255) gaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCt < 1:983995/150‑1 (MQ=255) aaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCtg > 1:1000790/1‑150 (MQ=255) gTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCgctgct > 2:692600/1‑150 (MQ=255) aCTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCtgctgc < 2:494668/150‑1 (MQ=255) aCTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCtgctgc < 2:494863/150‑1 (MQ=255) ttCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACt < 2:375214/95‑1 (MQ=255) ttCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACt > 1:375214/1‑95 (MQ=255) ggCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAg > 2:503801/1‑97 (MQ=255) ggCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAg < 1:503801/97‑1 (MQ=255) ccatcaggTGGTCGTCGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAAccc > 1:496889/8‑150 (MQ=255) ccatcaggTGGTCGTCGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAAccc < 2:496889/143‑1 (MQ=255) cAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGTTACATGCCGGTGCTGCGCAACCCGGa > 2:238702/1‑150 (MQ=255) tCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGc > 1:903024/1‑116 (MQ=255) tCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGc < 2:903024/116‑1 (MQ=255) gacgacCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGaaa < 2:341227/150‑1 (MQ=255) | AAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACATGCCGCTGCTGCGCAACCCGGACAAATCGAAA > NC_002947/2240283‑2240567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |