| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,263,099 | G→T | 6.8% | L257L (CTG→CTT) | truA → | tRNA pseudouridine (38‑40)synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,263,099 | 0 | G | T | 6.8% | 182.0 / 2.4 | 59 | L257L (CTG→CTT) | truA | tRNA pseudouridine (38‑40)synthase |
| Reads supporting (aligned to +/- strand): ref base G (26/29); new base T (2/2); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01 | |||||||||||
CCTTCCTGCACCACATGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGCCCTTTCGCCGCAACAATCAGGGTTCTT > NC_002947/2262951‑2263245 | cctgcctgCACCACATGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGTAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTgg < 2:195967/150‑1 (MQ=255) ttCCTGCACCACATGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTg > 2:62592/1‑150 (MQ=255) ccTGCACCACATGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCa < 2:670904/150‑1 (MQ=255) ccTGCACCACATGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCa < 2:189542/150‑1 (MQ=255) cTGCACCACATGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAg > 2:846529/1‑150 (MQ=255) caccacATGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTg < 1:606057/150‑1 (MQ=255) tGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTAcc < 2:693104/150‑1 (MQ=255) tGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACAGCCGTGAAGGTGGGGTGACGGCCCACCCAAACGAGCTTTACCGGGTGCAGGTGGAGTAcc > 2:900225/1‑150 (MQ=255) ggTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGGGCGTCCGGGTGCCTGGGCACGCGAGGGCCTGGAAGCGCGGAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGTAGTAccc > 1:809762/1‑150 (MQ=255) aCATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGt > 2:28058/1‑150 (MQ=255) aTTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGATGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGttt > 1:270209/1‑150 (MQ=255) gCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGtt < 2:391382/146‑1 (MQ=255) gCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGtt > 1:391382/1‑146 (MQ=255) gtgCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGccc < 2:153785/150‑1 (MQ=255) tgCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGc < 1:129850/142‑1 (MQ=255) tgCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGc > 2:129850/1‑142 (MQ=255) gATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTg < 2:840498/137‑1 (MQ=255) gATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTg > 1:840498/1‑137 (MQ=255) tGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGtt < 1:399462/150‑1 (MQ=255) ggCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATTCGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGGGTTTGCGTTGCCCAAGAGTTa < 2:1003727/150‑1 (MQ=255) ggCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGc < 1:696132/150‑1 (MQ=255) gcgcCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGcc > 1:611286/1‑150 (MQ=255) ggCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGc < 1:624517/127‑1 (MQ=255) ggCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGc > 2:624517/1‑127 (MQ=255) gTCCGGTTGCCTGGGCCCGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACATTTTTTGa > 2:569425/1‑150 (MQ=255) gTCCGGTTGCCTGGGCACGCGAGGTGCCGGGAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGa < 1:447022/150‑1 (MQ=255) ggTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTgg > 1:571933/1‑150 (MQ=255) tGGGCACGCGAGGTGCTGGAAGGGCGCAGCCGCCGTGAAGGTGGGGTAACGGCCCAGCCATACGGGCTTTACCTGGTGCAGGTAGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGtt > 2:584088/1‑118 (MQ=255) tGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑Ag > 2:313999/1‑150 (MQ=255) tGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTAGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGtt < 1:584088/118‑1 (MQ=255) gggCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑Agg < 1:846529/150‑1 (MQ=255) gcgAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTTGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGCGTgg > 2:357155/1‑136 (MQ=255) gcgAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTTGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTgg < 1:357155/136‑1 (MQ=255) gcgAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTTGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTgg > 2:357224/1‑136 (MQ=255) gcgAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTTGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTgg < 1:357224/136‑1 (MQ=255) tcgcgTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGc > 1:642554/5‑150 (MQ=255) cgAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGc < 2:809762/150‑1 (MQ=255) tGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACt > 2:1038419/1‑150 (MQ=255) tGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGtt > 1:520290/1‑91 (MQ=255) tGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGtt < 2:520290/91‑1 (MQ=255) tGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGAc < 2:400754/150‑1 (MQ=255) gAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGa < 2:270209/150‑1 (MQ=255) gcgcAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGaa > 1:640741/1‑150 (MQ=255) gcAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGtt < 2:1070529/130‑1 (MQ=255) gcAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGtt > 1:1070529/1‑130 (MQ=255) gcAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACGGACGAGCGaaaa < 2:831424/150‑1 (MQ=255) gccgTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCAtt < 2:642554/150‑1 (MQ=255) ccgTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCAttt < 2:611286/150‑1 (MQ=255) aGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAAc < 2:915722/150‑1 (MQ=255) ggTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGc > 2:512142/1‑150 (MQ=255) ccacccaTACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGCCCTTTcgcc > 2:629119/1‑150 (MQ=255) ccacccaTACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGCCCTTTcgcc > 2:78267/1‑150 (MQ=255) ccacccaTACGGCCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCt > 2:767905/1‑132 (MQ=255) ccacccaTACGGCCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCt < 1:767905/132‑1 (MQ=255) acccaTACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGCCCTTTcgccgc > 2:451068/1‑150 (MQ=255) acccaTACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGCCCTTTcgccgc < 1:451068/150‑1 (MQ=255) ggCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGA‑‑GCACACGTCTGACGTGGGCAGACTGACGAGGGAAAAGACCTTTGCCAGTATCCTCCCTCTCGCCGCAACAATCAg > 1:20536/1‑150 (MQ=255) gCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGCCCTTTCGCCGCAACAATCAgg < 1:62592/150‑1 (MQ=255) cTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGCCCTTTCGCCGCAACAATCAggg < 1:313999/150‑1 (MQ=255) ccTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGCCCTTTCGCCGCAACAATCAGGGTTCtt < 1:227821/150‑1 (MQ=255) | CCTTCCTGCACCACATGGTGCGTAACATTGCCGGTGTGCTGATGGCCATCGGCGCCGGCGAGCGTCCGGTTGCCTGGGCACGCGAGGTGCTGGAAGGGCGCAACCGCCGTGAAGGTGGGGTAACGGCCCACCCATACGGGCTTTACCTGGTGCAGGTGGAGTACCCCGAAGCGTTTGCGTTGCCCAAGCGTTACATCGGCCCACACTTTTTGAGTGGTTACG‑‑AGGCGTTGGCAGACTGACGAGCGAAAAGCCATTTGCTAACATCCGCCCTTTCGCCGCAACAATCAGGGTTCTT > NC_002947/2262951‑2263245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |