Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,315,536 G→A 5.8% L153L (CTC→CTT)  PP_2037 ← aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,315,5360GA 5.8% 203.1 / 2.3 69L153L (CTC→CTT) PP_2037aldolase
Reads supporting (aligned to +/- strand):  ref base G (37/28);  new base A (2/2);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

AACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTACAGCCAGCTGTGG  >  NC_002947/2315387‑2315683
                                                                                                                                                     |                                                                                                                                                   
aaCAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGAGGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTg                                                                                                                                                     >  2:1082425/1‑150 (MQ=255)
        agcgagcACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGc                                                                                                                                             <  1:342345/150‑1 (MQ=255)
          cgagcACGCAGGCTTCCTCGCTCGAACGTACACTGTTTCGCTAGACGTAGTTTGTAAGCAGGAGTGCGCGCGTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGcac                                                                                                                                           <  2:851497/150‑1 (MQ=255)
                      cTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACa                                                                                                                               <  1:267794/150‑1 (MQ=255)
                          cTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCTCCAATCTCGTTCGCGATGATTTGGCCGTCTTCGTTGCCCACCGGTACACCGGACCATTCCTTGAGGAATGCACAGTCCCCGAACAgggg                                                                                                                           >  1:228053/1‑150 (MQ=255)
                          cTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATGGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAgggg                                                                                                                           >  1:864979/1‑150 (MQ=255)
                           tcgatcgaACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAggggg                                                                                                                          <  2:254999/150‑1 (MQ=255)
                           tcgatcgaACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCCAGCCATTCCTTGAGGAATGCACAGTCGTCGAACAggggg                                                                                                                          >  1:789540/1‑150 (MQ=255)
                           tcgatcgaACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGACTTATTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAggggg                                                                                                                          >  1:685647/1‑150 (MQ=255)
                                  aaCGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGt                                                                                                                   <  1:1069535/150‑1 (MQ=255)
                                      cccAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCAt                                                                                                               >  2:174817/1‑150 (MQ=255)
                                      cccAGCGATCAGAAGGCCATGCTGGGAAAGAAGGATTGCGCGGCTGTCGACCCAGGCGTTAGAGCTGATTTCGACTTCTTCGTTTCCCACCTGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCAt                                                                                                               <  2:789540/150‑1 (MQ=255)
                                          gCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAg                                                                                                                     <  1:101007/140‑1 (MQ=255)
                                          gCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAg                                                                                                                     >  2:101007/1‑140 (MQ=255)
                                                  cagGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGc                                                                                                   >  1:1095837/1‑150 (MQ=255)
                                                   agGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGcc                                                                                                  <  2:100302/150‑1 (MQ=255)
                                                     gCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACACGTCCATGTGCGAGATGGCCAg                                                                                                >  2:1104952/1‑150 (MQ=255)
                                                      ccATGATGGGACAGCAGGATTGCGCGCTGGTCGCCACTCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCTGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGc                                                                                               <  2:142032/150‑1 (MQ=255)
                                                      ccATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTGGAACAGGGGGCACAGGTCCATGTGCgag                                                                                                        <  1:1051078/141‑1 (MQ=255)
                                                      ccATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCgag                                                                                                        >  2:1051078/1‑141 (MQ=255)
                                                       cATGATGGGATAAGAGGATTGCGTGCTTGTCGCCAATCGCGTGCGCGATGGTTTCTCCTTCTTCGTTACCCACCGGTACACCGGGCCATTCCTTGAGGAATGCGCAGTCGGCGAACAGGGGGCACAGGTCCATGTGCGAGGTGGCCAGCg                                                                                              >  2:183492/1‑150 (MQ=255)
                                                       cATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCCAAGGGACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCg                                                                                              >  1:688209/1‑150 (MQ=255)
                                                             gggAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACtt                                                                                        >  2:198115/1‑150 (MQ=255)
                                                               gAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTCAAGGAATggcc                                                                                                                                           <  1:371306/97‑4 (MQ=255)
                                                               gAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTCAAGGAATggcc                                                                                                                                           >  2:371306/1‑94 (MQ=255)
                                                                aaaGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCa                                                                                     >  1:780053/1‑150 (MQ=255)
                                                                aaaGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACGTcta                                                                                     <  2:803871/150‑3 (MQ=255)
                                                                  aGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGc                                                                                   <  2:612234/150‑1 (MQ=255)
                                                                    cAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATTCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCAt                                                                                 >  1:565956/1‑150 (MQ=255)
                                                                    cAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCAt                                                                                 >  2:590459/1‑150 (MQ=255)
                                                                       gATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACCGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGa                                                                              >  2:63112/1‑150 (MQ=255)
                                                                            cgcgcTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAgg                                                                         <  2:780053/150‑1 (MQ=255)
                                                                            cgcgcTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGGCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAgg                                                                         >  1:132565/1‑150 (MQ=255)
                                                                                    tCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCtg                                                                 >  2:692547/1‑150 (MQ=255)
                                                                                      gCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTCAAGGAATggcc                                                                                                                                           >  2:592915/1‑71 (MQ=255)
                                                                                      gCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTCAAGGAATggcc                                                                                                                                           <  1:592915/74‑4 (MQ=255)
                                                                                       ccAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGc                                                              >  2:376834/1‑150 (MQ=255)
                                                                                         aaTCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAg                                                            >  2:1028305/1‑150 (MQ=255)
                                                                                         aaTCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACACTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAg                                                            >  2:370529/1‑150 (MQ=255)
                                                                                          aTCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGc                                                           <  1:1028305/150‑1 (MQ=255)
                                                                                               gTTCGCGATGATTTCGCCTTCTTCGTTGCCCAACGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCgggtg                                                      <  2:688209/150‑1 (MQ=255)
                                                                                                ttCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCgggtgg                                                     <  1:174817/150‑1 (MQ=255)
                                                                                                   gcgATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGggtgggtg                                                  >  1:1025393/1‑150 (MQ=255)
                                                                                                     gatgatTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCga                                                                                                         <  1:458855/93‑1 (MQ=255)
                                                                                                     gatgatTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCga                                                                                                         >  2:458855/1‑93 (MQ=255)
                                                                                                     gatgatTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGtgtg                                                >  2:844447/1‑150 (MQ=255)
                                                                                                     gatgatTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGagtg                                                >  1:952753/1‑150 (MQ=255)
                                                                                                        gatTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACCGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGgat                                             <  1:63112/150‑1 (MQ=255)
                                                                                                         atTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGgatg                                            >  2:384219/1‑150 (MQ=255)
                                                                                                           ttCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGgatgat                                          >  1:294545/1‑150 (MQ=255)
                                                                                                            tCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGatgatg                                         <  2:294545/150‑1 (MQ=255)
                                                                                                               ccTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAg                                      >  2:955711/1‑150 (MQ=255)
                                                                                                                 ttcttcGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGCTATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGtt                                    <  2:266534/150‑1 (MQ=255)
                                                                                                                  tcttcGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAgggg                                                                                                                           >  1:468918/1‑62 (MQ=255)
                                                                                                                  tcttcGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAgggg                                                                                                                           <  2:468918/62‑1 (MQ=255)
                                                                                                                    ttcGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGAc                                 <  2:165759/150‑1 (MQ=255)
                                                                                                                    ttcGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGAc                                 >  1:1053809/1‑150 (MQ=255)
                                                                                                                       gTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGtg                              >  1:108720/1‑149 (MQ=255)
                                                                                                                           cccACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCgggg                          >  1:360064/1‑150 (MQ=255)
                                                                                                                            ccACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAAGAGGGGGCACAGGTCCATGTGCGAGATGGACAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGgggc                         <  2:228053/150‑1 (MQ=255)
                                                                                                                            ccACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGgggc                         >  1:90719/1‑150 (MQ=255)
                                                                                                                            ccACCGGTACACAGGGCCATTCCTTGAGGAATTCACAGTCGGCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGACATTCCACCATCGACAAGGTGGCGCGGGGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGgggc                         >  2:799935/1‑150 (MQ=255)
                                                                                                                              aCCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGgggcgg                       <  2:1095837/150‑1 (MQ=255)
                                                                                                                                      cccGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTa               <  1:183492/149‑1 (MQ=255)
                                                                                                                                          tgCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGCTCAAGTTGACGTCGGGGCGGGCGCGGTAcagc           <  1:759333/149‑1 (MQ=255)
                                                                                                                                          cgCCATTCCTTGAGGAATGCACAGTCGTCCAACAGGCGGCACAGGTCCATGTGCGAGAGGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCCGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTAcagc           <  1:1087369/149‑1 (MQ=255)
                                                                                                                                            ccATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGGGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTAcagcca         >  2:759333/1‑150 (MQ=255)
                                                                                                                                             cATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTAcagccag        >  1:532715/1‑150 (MQ=255)
                                                                                                                                               ttCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTACAGCCAGCt      <  1:590459/150‑1 (MQ=255)
                                                                                                                                                   ttGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTACAGCCAGCTGTgg  >  1:559320/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCATGATGGGAAAGCAGGATTGCGCGCTTGTCGCCAATCGCGTTCGCGATGATTTCGCCTTCTTCGTTGCCCACCGGTACACCGGGCCATTCCTTGAGGAATGCACAGTCGTCGAACAGGGGGCACAGGTCCATGTGCGAGATGGCCAGCGGAACTTCCAGCATCGACAAGGCGGCGCTGTGCAGCGGGTGGGTGTGGATGATGCAGTTGACGTCGGGGCGGGCGCGGTACAGCCAGCTGTGG  >  NC_002947/2315387‑2315683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑