Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,395,630 G→C 20.4% intergenic (‑195/+48) dacB ← / ← PP_2099 D‑alanyl‑D‑alanine carboxypeptidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,395,6300GC20.4% 97.8 / 17.6 44intergenic (‑195/+48)dacB/PP_2099D‑alanyl‑D‑alanine carboxypeptidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (19/16);  new base C (4/5);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAGG  >  NC_002947/2395483‑2395772
                                                                                                                                                   |                                                                                                                                              
gTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATggg                                                                                                                                              <  1:909710/150‑1 (MQ=255)
 tGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCAACAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATgggg                                                                                                                                             <  1:1081577/150‑1 (MQ=255)
        ccTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCATGTCGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtt                                                                                                                                      <  1:1091816/150‑1 (MQ=255)
            cggcgAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCa                                                                                                                                  <  1:498557/150‑1 (MQ=255)
                 aGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAAt                                                                                                                             >  2:348405/1‑150 (MQ=255)
                    gAAAAGGGCTTTGGGGCTGTAAGAGAGGATGGCCCCACGACGTAAACAGTGCGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCaa                                                                                                                          <  1:929612/150‑1 (MQ=255)
                     aaaaGGGCTTTGGGGCTGTAAGAGATGATGGCCCCCCGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAAc                                                                                                                         <  2:781891/150‑1 (MQ=255)
                       aaGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCt                                                                                                                       <  1:983865/150‑1 (MQ=255)
                             tttGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGc                                                                                                                 >  1:918350/1‑150 (MQ=255)
                             tttGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtttt                                                                                                                                    <  1:589170/131‑1 (MQ=255)
                             tttGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtttt                                                                                                                                    >  2:589170/1‑131 (MQ=255)
                                       tAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGa                                                                                                       >  1:220964/1‑150 (MQ=255)
                                         agagATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACa                                                                                                     >  2:836445/1‑150 (MQ=255)
                                            gatgatGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGt                                                                                                  >  1:849763/1‑150 (MQ=255)
                                             atgatgGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTa                                                                                                 <  1:112545/150‑1 (MQ=255)
                                               gatgGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCGGCCGCGATGCGGCACGTTCGACGGATTAGGATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATc                                                                                               >  1:921084/1‑150 (MQ=255)
                                               gatgGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATc                                                                                               <  1:348405/150‑1 (MQ=255)
                                                 tgGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCAc                                                                                             >  1:938103/1‑150 (MQ=255)
                                                           acgTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgt                                                                                   <  2:938103/150‑1 (MQ=255)
                                                              tAAACAGTACGCATTATG‑CCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGccc                                                                                                               >  1:972002/1‑118 (MQ=255)
                                                              tAAACAGTACGCATTATG‑CCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGccc                                                                                                               <  2:972002/118‑1 (MQ=255)
                                                                       cggtatATGACCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCCCCCGAATGGGGTGTCCTTTTCAGCATTCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCtttt                                                                       <  2:443656/145‑1 (MQ=255)
                                                                                  ccAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATAACACGGCCTTGTTGATAGGCTTTTCCGGGTACCAg                                                            >  1:673801/1‑150 (MQ=255)
                                                                                   ccaGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTCACAAGTATCACTCAGCCTTGTTGATAGGCTTCTCCGGGTAccccg                                                           >  2:348795/3‑147 (MQ=255)
                                                                                          aGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCcag                                                    >  2:817002/1‑150 (MQ=255)
                                                                                          aGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCcag                                                    <  1:742955/150‑1 (MQ=255)
                                                                                                      tCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGAc                                        <  2:849763/150‑1 (MQ=255)
                                                                                                           ttCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGTAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGa                                   >  2:644489/1‑150 (MQ=255)
                                                                                                           ttCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGc                                                                           >  1:781747/1‑110 (MQ=255)
                                                                                                           ttCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGc                                                                           <  2:781747/110‑1 (MQ=255)
                                                                                                                       gCATCGCCAATAAAAAGTCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGtt                       >  1:38059/1‑150 (MQ=255)
                                                                                                                          tCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGc                    >  1:803577/1‑150 (MQ=255)
                                                                                                                          tCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGc                    <  2:803577/150‑1 (MQ=255)
                                                                                                                             ccAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCt                                                                          >  2:473329/1‑93 (MQ=255)
                                                                                                                             ccAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCt                                                                          <  1:473329/93‑1 (MQ=255)
                                                                                                                              cAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCg                >  1:975236/1‑150 (MQ=255)
                                                                                                                                aTAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGc              <  2:220964/150‑1 (MQ=255)
                                                                                                                                 tgaaaaGGCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGAc                                        >  2:338283/3‑123 (MQ=39)
                                                                                                                                 tgaaaaGGCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGAc                                        <  1:338283/121‑1 (MQ=255)
                                                                                                                                     aaGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTg         >  1:994972/1‑150 (MQ=255)
                                                                                                                                     aaGGCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTg         >  1:844460/1‑150 (MQ=255)
                                                                                                                                      aGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGa        >  2:829669/1‑150 (MQ=255)
                                                                                                                                      aGGCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGa        <  2:844460/150‑1 (MQ=255)
                                                                                                                                        gCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATa                                                                              >  2:333754/1‑78 (MQ=38)
                                                                                                                                        gCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATa                                                                              <  1:333754/78‑1 (MQ=38)
                                                                                                                                            cccGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAgg  <  2:38059/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCCAGG  >  NC_002947/2395483‑2395772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑