| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,412,947 | G→C | 13.1% | intergenic (+168/‑253) | rlmM → / → PP_2114 | 23S rRNA (cytidine(2498)‑2'‑O)‑methyltransferase RlmM/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,412,947 | 0 | G | C | 13.1% | 115.9 / 4.6 | 43 | intergenic (+168/‑253) | rlmM/PP_2114 | 23S rRNA (cytidine(2498)‑2'‑O)‑methyltransferase RlmM/transposase |
| Reads supporting (aligned to +/- strand): ref base G (18/19); new base C (3/3); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCC > NC_002947/2412799‑2413095 | ttctgcGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatgg > 1:804288/1‑150 (MQ=255)ttctgcGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatgg > 2:1079197/1‑150 (MQ=255) ctgcgtctgcgtCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatggat < 2:1047510/150‑1 (MQ=255) ctgcgtctgcgtCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatggat < 1:46553/150‑1 (MQ=255) tgcgtctgcgtcTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatggatg > 1:754367/1‑150 (MQ=255) tctgcgtctgcTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCATCAAGCGCTG‑AGGCCCCATGGATgggga > 2:10342/1‑149 (MQ=255) tctgcttctgctTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAg < 2:277868/150‑1 (MQ=255) ctgcttctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAgc > 1:905020/1‑150 (MQ=255) tgcttctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATCCATGGGGCCT‑CAgcgc < 1:33615/150‑1 (MQ=255) ttctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatggatgg > 2:687610/1‑136 (MQ=255) ttctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCatggatgg < 1:687610/136‑1 (MQ=255) ttctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGt > 2:573209/1‑150 (MQ=255) ttctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGt > 1:1113701/1‑150 (MQ=255) tgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCACGCGCTGCAGGCCCCATCCATGGGGCCT‑CAgcgcttgac < 2:42963/150‑6 (MQ=14) cttTTGCTGTTGCTTCTAAGCGCGGAGGAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTcc > 1:705199/1‑150 (MQ=255) ttGGTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg < 2:877227/150‑1 (MQ=37) gCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGGTCGGCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGa < 1:923000/150‑1 (MQ=255) gCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGa < 2:452823/150‑1 (MQ=38) ttGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCTCCATCTATTTGGCCTGCAGCGCGTACTCCCGGGAGCaa > 2:88679/1‑150 (MQ=11) ttCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCACGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGccc > 2:903107/1‑150 (MQ=38) tAAGCGTGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGTAAGCCCGGa < 1:857214/150‑1 (MQ=255) gcgcgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCAGGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAg < 2:1113701/150‑1 (MQ=38) aCGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAg > 2:946744/1‑150 (MQ=38) aGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCa > 2:1105308/1‑150 (MQ=39) ttGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCggg > 1:844099/1‑109 (MQ=33) ttGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCggg < 2:844099/109‑1 (MQ=33) gCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGc > 1:293402/1‑150 (MQ=39) aggaggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCAAGGCTGAGCCTCCAGCGCTG‑AGGCCCCATCAGCGGGGCCTCCAGCGCATACTCTCGGCGGTAAGCCCGGGGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGgatcag > 2:401788/1‑147 (MQ=11) ggaggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTATTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATa < 1:153394/150‑1 (MQ=38) ggaggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATa > 2:371417/1‑150 (MQ=39) ggaggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATa > 2:427759/1‑150 (MQ=38) gaggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTa > 1:612155/1‑89 (MQ=33) gaggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTa < 2:612155/89‑1 (MQ=35) tAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGt > 1:878861/1‑150 (MQ=255) ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTg < 1:573209/150‑1 (MQ=39) gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTa > 1:914766/1‑150 (MQ=40) aaGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTAc < 2:118829/150‑1 (MQ=40) ggAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCAAGATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACttt < 1:10342/150‑1 (MQ=255) gTGACGGGCAGGGATGCCCGTCAAGCGCTG‑CGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGt < 2:1053441/150‑1 (MQ=255) tGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTc < 2:804288/150‑1 (MQ=40) ggCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGtt < 2:878861/150‑1 (MQ=40) ggCAGGGATCCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGGGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGtt < 1:620846/150‑1 (MQ=255) ccGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGt > 2:716470/1‑150 (MQ=40) ccGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACGCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGt < 1:906051/150‑1 (MQ=255) gTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGa < 2:293402/150‑1 (MQ=40) gTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGa < 1:1079197/150‑1 (MQ=40) tgcaGGCCCCATCCATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTAc > 2:116166/4‑116 (MQ=255) tgcaGGCCCCATCCATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTAc < 1:116166/113‑1 (MQ=255) ccATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCa > 2:1079168/1‑150 (MQ=255) ccATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCa > 1:717326/1‑150 (MQ=255) tggatggGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATcc < 2:717326/150‑1 (MQ=255) | TTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑AGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCC > NC_002947/2412799‑2413095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |