| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,444,458 | A→C | 18.4% | *159G (TGA→GGA) | sulA ← | cell division inhibitor SulA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,444,458 | 0 | A | C | 18.4% | 73.4 / 12.2 | 37 | *159G (TGA→GGA) | sulA | cell division inhibitor SulA |
| Reads supporting (aligned to +/- strand): ref base A (15/15); new base C (3/4); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01 | |||||||||||
GGCCAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGC > NC_002947/2444312‑2444603 | ggCCAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGcc > 1:418498/1‑150 (MQ=255) cATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGAt > 2:833583/1‑150 (MQ=255) cATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCGGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAG‑AGCGGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGAt < 2:1122126/150‑1 (MQ=255) cAGTGTGGGCGTGCAGATGGCCGGTCGCGTGATGGATGGCGAGCTCGGGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGTAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGTCGAGGCGGATGTTCAg < 2:55315/150‑1 (MQ=255) tgGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCttt > 1:469222/1‑150 (MQ=255) ggCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACCTCCGCCGAGGCGGATGTTCAGGCTTtgt < 1:996783/150‑1 (MQ=255) ggCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTtgt < 2:418498/150‑1 (MQ=255) tGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCAc > 2:809028/1‑150 (MQ=255) gatggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGc < 1:487761/150‑1 (MQ=255) tggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAg > 2:767512/1‑150 (MQ=255) cTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCt > 1:651510/1‑132 (MQ=255) cTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCt < 2:651510/132‑1 (MQ=255) tCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGc < 1:809028/150‑1 (MQ=255) gAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCCGCCTCGGCTGATGTTCa > 2:652288/1‑103 (MQ=38) gAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCCGCCTCGGCTGATGTTCa < 1:652288/103‑1 (MQ=38) gTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAg > 1:1002740/1‑150 (MQ=255) gatgaAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCg < 2:1118738/150‑1 (MQ=255) aGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCAtt < 2:823717/150‑1 (MQ=255) ggCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑TTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGa < 1:964951/150‑1 (MQ=255) ggCCGCGTGTGCTGCACGGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCGTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGa < 2:332283/150‑1 (MQ=255) tgctgcACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCcca < 2:1002740/150‑1 (MQ=255) aCTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCCGCCTCGGCTGATGTTCa > 1:893844/1‑75 (MQ=21) aCTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCCGCCTCGGCTGATGTTCa < 2:893844/75‑1 (MQ=21) aGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTcac > 2:990494/1‑150 (MQ=255) cagcagC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgt > 2:311682/1‑150 (MQ=255) gc‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGATCATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGc > 2:649096/1‑150 (MQ=255) tATCAGGCTATTTGAAGTGACAAGCAAAGCCTGAACATCAGCCGAGCCGCATGTTCAGGCTTTGTGCTTTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGa < 1:860479/150‑1 (MQ=255) ggCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGccc > 2:6639/1‑150 (MQ=255) gCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCa > 2:367521/1‑150 (MQ=255) cTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAg < 1:502177/150‑1 (MQ=255) aCAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAg > 2:564798/1‑132 (MQ=255) aCAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAg < 1:564798/132‑1 (MQ=255) ccTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGt > 2:370640/1‑150 (MQ=255) cTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGtt > 2:979384/1‑150 (MQ=255) tGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCATGCAGCGGCATTGACGTTGCcca < 2:249299/95‑1 (MQ=25) tGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCATGCAGCGGCATTGACGTTGCcca > 1:249299/1‑95 (MQ=37) cATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAgc < 2:175959/150‑1 (MQ=255) | GGCCAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGC‑GTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGC > NC_002947/2444312‑2444603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |