| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,447,461 | G→C | 12.5% | L269L (CTG→CTC) | nagZ → | beta‑N‑acetylglucosaminidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,447,461 | 0 | G | C | 12.5% | 112.9 / 8.9 | 40 | L269L (CTG→CTC) | nagZ | beta‑N‑acetylglucosaminidase |
| Reads supporting (aligned to +/- strand): ref base G (16/19); new base C (3/2); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
ACCAGCCCGCTGGCTTCTCGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCAAGGGTTTGCGCGAACCGATTATCGC > NC_002947/2447316‑2447605 | aCCAGCCCGCTGGCTTCTCGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAgcg > 2:637738/1‑150 (MQ=255) aGCCCGCTGGCTTCTCGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCg < 2:188725/150‑1 (MQ=255) aGCCCGCTGGCTTCTCGCGGCGTTGGTTGCAGGACATCCTGCGGGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCg > 2:714731/1‑150 (MQ=255) cccGCTGGCTTCTCGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCAGTATCGAGGCGGCGCTCAGCGCCgcc < 1:204091/150‑3 (MQ=255) gCTGGCTTCTCGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTtgt < 2:973845/150‑1 (MQ=255) gCTTCTCGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGata < 2:892646/150‑1 (MQ=255) ttCTCGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATg < 2:145904/150‑1 (MQ=255) tctcGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATgg < 2:801126/150‑1 (MQ=255) tcGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGc < 1:223672/150‑1 (MQ=255) gcggcgTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCt > 1:240555/1‑150 (MQ=255) gttgCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCaa > 1:1067131/1‑150 (MQ=255) ttgCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAAt > 1:1064877/1‑150 (MQ=255) ttgCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGAGCTGAGCGCAGGGTGTTATATGGGCCTGGTGTGCAAt > 2:289187/1‑150 (MQ=255) ccTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGggcggc < 1:937841/150‑1 (MQ=255) aGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTgg < 2:1012470/150‑1 (MQ=255) gCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGgc < 2:547042/150‑1 (MQ=255) gCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGGCGCGGCCAGCCGTATCGAGGCGGCGCTGGGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGAACGGGCGGCGGCGGAGCTGGCGCTGa < 2:196173/150‑1 (MQ=255) tttGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGt > 1:255813/1‑150 (MQ=255) ttGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTg < 2:1067131/150‑1 (MQ=255) aTGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTgcgg > 2:494894/1‑150 (MQ=255) tgaCCTGTCGATGGCCGGTGCGCATGTGGTGGGCGCCGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCaa < 1:289187/150‑1 (MQ=255) tgaCCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCaa < 2:588645/150‑1 (MQ=255) aCCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGACGGTCAAGc > 2:562990/1‑150 (MQ=255) ccTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGcc < 2:255813/150‑1 (MQ=255) ccGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGa < 1:723752/150‑1 (MQ=255) cgcATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTgcgc < 1:637738/150‑1 (MQ=255) gACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCaa > 2:853730/1‑150 (MQ=255) cAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCAAGGGTTTgc > 1:1022287/1‑150 (MQ=255) tATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCAAGGGTTTGCGCGAAc > 2:144502/1‑150 (MQ=255) gAGGCGGCGCTGAGCGCCGGTAGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGGGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGCATTGCGCGGATGCGTTGGCAAGGGGTTGCGCGAACCGAt > 2:398662/1‑150 (MQ=255) aaccggcgCTCAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCAAGGGTTTGCGCGAACCGAtt > 1:802882/4‑150 (MQ=255) accggcgCTCAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCAAGGGTTTGCGCGAACCGATTa > 2:829665/3‑150 (MQ=255) gcggcgCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCTGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGAtt < 2:1065400/111‑1 (MQ=255) gcggcgCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCTGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGAtt > 1:1065400/1‑111 (MQ=255) gcggcgCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCAAGGGTTTGCGCGAACCGATTAt > 1:836344/1‑150 (MQ=255) ccggcgCTCAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAg > 1:890240/2‑90 (MQ=255) ccggcgCTCAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAg < 2:890240/89‑1 (MQ=255) ggcgCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGgcgcagc > 1:875494/1‑80 (MQ=255) ggcgCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGgcgcagc < 2:875494/80‑1 (MQ=255) gcgcTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCAAGGGTTTGCGCGAACCGATTATCGc < 2:240555/150‑1 (MQ=255) gAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCAAGGGTTTGCGCGAACCGa < 1:581037/138‑1 (MQ=255) gAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGAAAGGGTTTGCGCGAACCGa > 2:581037/1‑138 (MQ=255) | ACCAGCCCGCTGGCTTCTCGCGGCGTTGGTTGCAGGACATCCTGCGTGGCGAGCTGGGCTTTGATGGGGTGATTTTCAGTGATGACCTGTCGATGGCCGGTGCGCATGTGGTGGGCGACGCGGCCAGCCGTATCGAGGCGGCGCTGAGCGCCGGTTGTGATATGGGCCTGGTGTGCAATGACCGGGCGGCGGCGGAGCTGGCGCTGAGTGCGGCGCAGCGCATGAAGGTCAAGCCATCGCCGCGGATTGCGCGGATGCGTGGGCAAGGGTTTGCGCGAACCGATTATCGC > NC_002947/2447316‑2447605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |