Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,711,029 T→G 9.1% intergenic (+289/+25) PP_2373 → / ← PP_2374 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,711,0290TG 9.1% 120.3 / 2.6 51intergenic (+289/+25)PP_2373/PP_2374hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/26);  new base G (3/2);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTCGCGTGCGCGGT  >  NC_002947/2710880‑2711178
                                                                                                                                                     |                                                                                                                                                     
tACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGtt                                                                                                                                                       <  2:1024575/150‑1 (MQ=255)
   ccGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCAc                                                                                                                                                    >  2:1080212/1‑150 (MQ=255)
            ggccgggccTGCTGGCCAATTTCCCCCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGAGTTGTATACAGTCCGCTGTAGGCGCGGGTTCACCCGCGAaga                                                                                                                                           <  1:694028/150‑1 (MQ=21)
             gccgggccTGCTGGCCAATTTCCACCTGCCGTTCAACCTAAGCACCGCCTTCGCGTTCGCGGGTAAACCCGCACCGGCGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAagag                                                                                                                                          <  1:1097696/150‑1 (MQ=21)
               cgggccTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGc                                                                                                                                        <  1:140357/150‑1 (MQ=255)
                  gccTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCgg                                                                                                                                     >  2:670745/1‑150 (MQ=255)
                        tgGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAgg                                                                                                                               >  1:693329/1‑150 (MQ=255)
                          gCCAATTTCCACCTGCCGTTCATCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTGAACCcgcgc                                                                                                                                              <  2:858327/133‑2 (MQ=255)
                          gCCAATTTCCACCTGCCGTTCATCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTGAACCcgcgc                                                                                                                                              >  1:858327/1‑132 (MQ=255)
                            cAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAg                                                                                                                           >  2:659426/1‑150 (MQ=255)
                               tttCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCgg                                                                                                                        <  1:562772/150‑1 (MQ=255)
                                    cccTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAg                                                                                                                   <  2:719411/149‑1 (MQ=255)
                                    aCCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGTTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAg                                                                                                                   <  1:287608/150‑1 (MQ=255)
                                    aCCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCCGGAGGCGCGGGCTCACCCCCGAAGAGGCCGGCACAGGTTAGCGGGTCAg                                                                                                                   >  2:199954/1‑150 (MQ=255)
                                         ccGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCa                                                                                                              >  1:490590/1‑150 (MQ=255)
                                          cGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAg                                                                                                             <  2:30602/150‑1 (MQ=255)
                                          cGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAg                                                                                                             >  1:373165/1‑150 (MQ=255)
                                           gTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAgg                                                                                                            <  2:984726/150‑1 (MQ=255)
                                           gTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACAAAGGCCGCTGTGCGCCCGAGGTCACCCGGTTGGATGCCGGCACATGTTAGCGGGTCAGGTGCAgg                                                                                                            <  1:139715/150‑1 (MQ=18)
                                           gTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAAACCACTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAgg                                                                                                            <  1:850618/150‑1 (MQ=255)
                                                ccGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCaa                                                                                                       <  2:490590/150‑1 (MQ=255)
                                                    tGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGc                                                                                                   <  1:659426/150‑1 (MQ=255)
                                                            cTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTAGGGGGTTGGGGAGAGGCCGCTGGAGGCGCGGGTGCACCCGCGAGGAGGCCGGTACAGGTTAGCGGGTCAGGTGCAGGGCAAACTGCACCAGccc                                                                                           >  2:463222/1‑150 (MQ=25)
                                                             tACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCg                                                                                          >  2:994834/1‑150 (MQ=255)
                                                                  tATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCaa                                                                                     >  1:207945/1‑150 (MQ=255)
                                                                   aTTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAAt                                                                                    <  2:566156/150‑1 (MQ=255)
                                                                     tCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAgg                                                                                                            >  1:344316/1‑124 (MQ=255)
                                                                     tCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAgg                                                                                                            <  2:344316/124‑1 (MQ=255)
                                                                       gcgGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCAc                                                                                                 >  1:530438/1‑133 (MQ=255)
                                                                       gcgGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCAc                                                                                                 <  2:530438/133‑1 (MQ=255)
                                                                           gTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTGAACCcgcgc                                                                                                                                              <  2:878899/84‑2 (MQ=21)
                                                                           gTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTGAACCcgcgc                                                                                                                                              >  1:878899/1‑83 (MQ=255)
                                                                                 ccGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAacac                                                                      >  2:975357/1‑150 (MQ=255)
                                                                                   gCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCa                                                                    <  2:781889/150‑1 (MQ=255)
                                                                                     tCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAgg                                                                  >  1:524696/1‑150 (MQ=255)
                                                                                       cTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGt                                                                <  2:693329/150‑1 (MQ=255)
                                                                                          cGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGaa                                                             <  2:1024542/150‑1 (MQ=255)
                                                                                                 aCGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGt                                                                                                                              >  1:827077/1‑78 (MQ=255)
                                                                                                 aCGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGt                                                                                                                              <  2:827077/78‑1 (MQ=255)
                                                                                                      ggTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAg                                                 <  2:207945/150‑1 (MQ=255)
                                                                                                           acacAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCaa                                            <  2:373165/150‑1 (MQ=255)
                                                                                                               aGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGaa                                        >  2:531085/1‑150 (MQ=255)
                                                                                                                      gggTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGtggctg                                 >  2:964947/1‑150 (MQ=255)
                                                                                                                         ttGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGcc                              <  1:1080212/150‑1 (MQ=255)
                                                                                                                           ggACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCtt                            >  2:86396/1‑150 (MQ=255)
                                                                                                                              cacaGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGtggctgg                                <  1:294678/143‑1 (MQ=255)
                                                                                                                              cacaGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGtggctgg                                >  2:294678/1‑143 (MQ=255)
                                                                                                                                   gCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAGCTGCACCAGCCCGATCAATACCGCTATGAACACCTGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTggg                    <  1:634659/150‑1 (MQ=255)
                                                                                                                                        tGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAg               >  1:536337/1‑150 (MQ=255)
                                                                                                                                            ggCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTcgc           <  1:497271/150‑1 (MQ=255)
                                                                                                                                               gcgGGTTCACCCGCGAAGAATAGGTCATATGTGTGCTGGTCAGGGGGAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTCGCGTg        <  1:516118/150‑1 (MQ=255)
                                                                                                                                                   gTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTCGCGTGcgcg    >  1:893508/1‑150 (MQ=255)
                                                                                                                                                     tCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTCGCGTGCGCGGt  <  1:964947/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACCCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTCGCGTGCGCGGT  >  NC_002947/2710880‑2711178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑