| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 249,248 | T→C | 8.1% | Q200Q (CAA→CAG) | qmcA ← | membrane protease family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 249,248 | 0 | T | C | 8.1% | 137.6 / 2.6 | 49 | Q200Q (CAA→CAG) | qmcA | membrane protease family protein |
| Reads supporting (aligned to +/- strand): ref base T (23/22); new base C (2/2); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01 | |||||||||||
CACTATTTCTCCTTGTCCACCAACCCCTTCAGCAGATCTATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCTTGATCTCGACGTTGGCCACCTTGA > NC_002947/249099‑249396 | cACTATTTCTCCTTGTCCACCAACCCCTTCAGCAGATCTATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCGTATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCt < 2:497882/150‑1 (MQ=255) tcCTTGTCCACCAACCCCTTCAGCAGATCTATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGt > 1:750359/1‑150 (MQ=255) cTTGTCCACCAACCCCTTCAGCAGATCTATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGttt > 2:684643/1‑150 (MQ=255) caccaACCCCTTCAGCAGATCTATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGAGGCTTGCATCACTTTA‑‑TCCGa > 2:612794/1‑150 (MQ=255) caACCCCTTCAGCAGATCTATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGc > 1:413462/1‑150 (MQ=255) agcagATCTATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCa > 1:1075717/1‑150 (MQ=255) gcagATCTATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCAc > 2:938444/1‑150 (MQ=255) tATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGc > 2:847087/1‑150 (MQ=255) tCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCAt < 2:750359/150‑1 (MQ=255) ctctTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCgcgc < 1:847087/150‑1 (MQ=255) tctTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGct < 2:600266/150‑1 (MQ=255) gTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCCTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGTAt < 1:770800/119‑1 (MQ=255) gTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCCTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCAt < 1:214748/119‑1 (MQ=255) gTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCCTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCAt > 2:214748/1‑119 (MQ=255) gTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCAGCTCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCCTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCAt > 2:770800/1‑119 (MQ=255) tCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGctcggcc < 2:823351/150‑1 (MQ=255) ccGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGctcggcctcg > 2:659268/1‑150 (MQ=255) cGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGctcggcctcgg > 1:387871/1‑150 (MQ=255) caTCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTg < 1:706509/149‑1 (MQ=255) gTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTggcgg < 1:612794/150‑1 (MQ=255) tCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTggcggg > 1:1053036/1‑150 (MQ=255) tCCCCAGTGGCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTggcggg < 2:1024076/150‑1 (MQ=255) cccccAGTGTCTCCATATAGCGCAACTGCATCGCCCCCGATTCCTTACTC‑‑CTGACGCTCTCTTTGCTGATTTTTCCAACCGACGCCTGCAGTTCACCCTCGCCATGAATCACCCTGGCCCTACCTTCGCGCTCGGCCTCGGCCTggcggg < 1:684643/149‑1 (MQ=255) ccAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGa > 1:60964/1‑150 (MQ=255) gCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTa < 2:1075717/150‑1 (MQ=255) atatAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAAGCACCTTGGCCCGACGTTCGCGCTCGGCCCCGGCCTGGCGGGCGCTGGCGCGTAcc > 1:359307/1‑150 (MQ=255) cgcAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGAc > 1:360088/1‑150 (MQ=255) cAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCCCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTc < 1:541111/150‑1 (MQ=255) aaCTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCg > 1:563173/1‑150 (MQ=255) aCTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATc < 2:1102707/47‑1 (MQ=255) aCTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATc > 1:1102707/1‑47 (MQ=255) tGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTc < 1:678676/150‑1 (MQ=255) cATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAg < 1:51229/150‑1 (MQ=255) ccGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGt > 1:548271/1‑150 (MQ=255) gTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGGCGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCt < 1:237334/150‑1 (MQ=255) ttGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCTTGAtc > 1:1100203/1‑150 (MQ=255) ttGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCCCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCa > 2:333265/1‑133 (MQ=255) ttGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCCCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCa < 1:333265/133‑1 (MQ=255) tCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCTTGATCTCGa > 1:1061931/1‑150 (MQ=255) cAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCTTGCTCTCGAc > 1:491296/1‑150 (MQ=255) aGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGTGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAAc < 2:830246/134‑1 (MQ=255) aGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGTGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAAc > 1:830246/1‑134 (MQ=255) aGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCAGCCTCGGCCTggcggg > 1:353496/1‑100 (MQ=255) aGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCAGCCTCGGCCTggcggg < 2:353496/100‑1 (MQ=255) aTTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCTTGATCTCGACGTTg < 2:1053036/150‑1 (MQ=255) tttGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTc < 2:726143/73‑1 (MQ=255) tttGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTc > 1:726143/1‑73 (MQ=255) tGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCAGGAAGCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCTTGATCTCGACGTTGGcc < 2:413462/150‑1 (MQ=255) cgGCTTGCATCAGTTGC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCTTGATCTCGACGTTGGCCACCt < 2:360088/150‑1 (MQ=255) cTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCTTGATCTCGACGTTGGCCACCTTGa < 2:1100203/150‑1 (MQ=255) | CACTATTTCTCCTTGTCCACCAACCCCTTCAGCAGATCTATCGGCAGCGGGAAGACGATGGTCGAGCTCTTGTCCCCGGCAATCGTCCCCAGTGTCTGCATATAGCGCAACTGCATCGCCCCCGGTTCCTTGCTCAGCATTTGCGCGGCTTGCATCAGTTTC‑‑TCCGACGCCTGCAGTTCACCCTCGGCATGAATCACCTTGGCCCGACGTTCGCGCTCGGCCTCGGCCTGGCGGGCGATGGCGCGTACCATCGACTCGTTCAGGTCAACGTGCTTGATCTCGACGTTGGCCACCTTGA > NC_002947/249099‑249396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |