Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,926,936 A→G 7.3% T54A (ACT→GCT)  PP_2565 → UvrD/REP family helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,926,9360AG 7.3% 150.6 / 2.4 55T54A (ACT→GCT) PP_2565UvrD/REP family helicase
Reads supporting (aligned to +/- strand):  ref base A (27/24);  new base G (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

AACCCTTGGCCCCCGCCGACCTTACCCCTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACTTGTTCGACGACTGGC  >  NC_002947/2926790‑2927083
                                                                                                                                                  |                                                                                                                                                   
aaCCCTTGGCCCCCGCCGACCTTACCCCTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTg                                                                                                                                                  <  2:148914/150‑1 (MQ=255)
  cccTTGGCCCCCGCCGACCTTACCCCTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGTGCGCTGGc                                                                                                                                                <  2:161070/150‑1 (MQ=255)
  cccTTGGCCCCCGCCGACCTTACCCCTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGACGGAGGCTGACCCGCCTGCACGCCAAGCACCGCGCAGCATGGTTCCTGGGGCACGCCACAGGCCAGCGCACTGGc                                                                                                                                                >  1:519009/1‑150 (MQ=255)
       ggCCCCCGCCGACCTTACCCCTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGc                                                                                                                                           <  2:731541/150‑1 (MQ=255)
           cccGCCGACCTTACCCCTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGAt                                                                                                                                       >  1:964276/1‑150 (MQ=255)
                 gACCTTACCCCTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGgcgtg                                                                                                                                 >  1:609417/1‑150 (MQ=255)
                      tACCCCTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGa                                                                                                                            <  2:137887/150‑1 (MQ=255)
                         cccTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAgg                                                                                                                         >  1:1120014/1‑150 (MQ=255)
                                          ccTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCa                                                                                                                                              >  2:7355/1‑112 (MQ=255)
                                          ccTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCa                                                                                                                                              <  1:7355/112‑1 (MQ=255)
                                          ccTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCggg                                                                                                        <  2:482292/150‑1 (MQ=255)
                                           cTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGa                                                                                                                   <  2:274397/138‑1 (MQ=255)
                                           cTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGa                                                                                                                   >  1:274397/1‑138 (MQ=255)
                                           cTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGt                                                                                                       >  2:844137/1‑150 (MQ=255)
                                           cTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGt                                                                                                       >  1:487934/1‑150 (MQ=255)
                                            tGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTg                                                                                                      >  1:395912/1‑150 (MQ=255)
                                            tGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGAACCGCCTGCAAGCCCAGGACCGCGACTCATGGTTCCTCGGTCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTg                                                                                                      >  2:679476/1‑150 (MQ=255)
                                             ggTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTgg                                                                                                     <  2:964276/150‑1 (MQ=255)
                                              gTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGa                                                                                                    >  2:1061399/1‑150 (MQ=255)
                                              gTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGa                                                                                                    <  2:418513/150‑1 (MQ=255)
                                              gTTTCGCCGCCTGGACGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGACCACGCCACAGGCCAGCGCACGGGCCATGCCGATGGCGTCCGTGAAGGGTTCGAACGTAGTCAGGGGGa                                                                                                    <  2:454722/150‑1 (MQ=255)
                                                      gccTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTg                                                                                            >  2:688247/1‑150 (MQ=255)
                                                           gccgccCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCg                                                                                       >  1:882681/1‑150 (MQ=255)
                                                             cgcccgccTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCggg                                                                                     <  2:882681/150‑1 (MQ=255)
                                                              gcccgccTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCgggg                                                                                    >  1:620045/1‑150 (MQ=255)
                                                               cccgccTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGAGTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGgc                                                                                   <  1:200397/150‑1 (MQ=255)
                                                                    ctgctgGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAg                                                                              <  1:399886/150‑1 (MQ=255)
                                                                         ggggCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCt                                                                         <  1:200263/150‑1 (MQ=255)
                                                                          gggCGTGGGCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTg                                                                        <  2:609417/150‑1 (MQ=255)
                                                                            gCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGt                                                                      >  2:294416/1‑150 (MQ=255)
                                                                             cGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCATGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTg                                                                     <  2:603350/150‑1 (MQ=255)
                                                                                     gACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGa                                                             <  1:294416/150‑1 (MQ=255)
                                                                                        ccGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGGTGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGGGGAGGGCTTTGAAGCGGAGCGCCAGGTGCTGGCGGTCCGCGGTAc                                                          >  2:123831/1‑150 (MQ=255)
                                                                                              gCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCgg                                                                                      <  2:947935/116‑1 (MQ=255)
                                                                                              gCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCgg                                                                                      >  1:947935/1‑116 (MQ=255)
                                                                                                    ccAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGTGCGCCAAGTGCTGGTGATACGGGATACGCGCCCCGACAg                                              >  1:118963/1‑150 (MQ=255)
                                                                                                    ccAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGgc                                                                                   <  1:244383/113‑1 (MQ=255)
                                                                                                    ccAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGgc                                                                                   >  2:244383/1‑113 (MQ=255)
                                                                                                           cgcgACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCt                                       >  1:246668/1‑150 (MQ=255)
                                                                                                             cgACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCtgt                                     <  1:1061399/150‑1 (MQ=255)
                                                                                                                cTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGGCGCtgtcgg                                  <  2:709134/150‑4 (MQ=255)
                                                                                                                      gTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTgccaggcca                            >  2:252190/1‑150 (MQ=255)
                                                                                                                         ccTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGa                         <  2:620045/150‑1 (MQ=255)
                                                                                                                               ccacgccacAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACaa                   <  2:843790/150‑1 (MQ=255)
                                                                                                                                cacgccacAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGACGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAAc                  <  1:264570/150‑1 (MQ=255)
                                                                                                                                  cgccacAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACtt                >  1:206539/1‑150 (MQ=255)
                                                                                                                                    ggcatggCCAGTGCGCTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACTTGt              >  1:785549/6‑150 (MQ=255)
                                                                                                                                       atggCCAGTGCGCTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTgccag                                >  1:591785/3‑129 (MQ=255)
                                                                                                                                       atggCCAGTGCGCTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTgccag                                <  2:591785/127‑1 (MQ=255)
                                                                                                                                          gCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACTTGTTcgacg        >  2:475134/1‑150 (MQ=255)
                                                                                                                                             aGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACTTGTTCGACGACt     >  1:125488/1‑150 (MQ=255)
                                                                                                                                             aGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACTTGTTCGACGACt     >  1:240842/1‑150 (MQ=255)
                                                                                                                                             aGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACTTGATCGACGACt     >  1:888803/1‑150 (MQ=255)
                                                                                                                                                gcACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGGGCGTGGCCGGGTGGAGAGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATACGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACTTGTTCGACGACCGgc  >  2:18033/1‑150 (MQ=255)
                                                                                                                                                gcACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCTACCGGTTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACTTGTTCGACGACTGgc  <  1:688247/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AACCCTTGGCCCCCGCCGACCTTACCCCTCCTGCCCAGTTGCCCTGGTTTCGCCGCCTGGCCGCCCGCCTGCTGGGGCGTGGCCTGACCCGCCTGCAAGCCCAGCACCGCGACTCATGGTTCCTCGGCCACGCCACAGGCCAGCGCACTGGCCATGCCGATGGCGTGCGTGAAGGGTTCGAGCGTGGTCGGGTGGAGGGCTTTGAAGCGGGGCGCCAGGTGCTGGTGATCCGCGATACGCGCCCCGACAGCGCCGCTGTGCCAGGCCAGGATGACAACTTGTTCGACGACTGGC  >  NC_002947/2926790‑2927083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑