Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,965,867 C→T 7.7% G268D (GGC→GAC)  PP_2595 ← ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,965,8670CT 7.7% 147.6 / 2.7 52G268D (GGC→GAC) PP_2595ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (25/23);  new base T (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CTTGGCTGCCAGGATCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGCCCATGCCGCTGCTGCGCTGGCGCTCCAGGCTTT  >  NC_002947/2965719‑2966011
                                                                                                                                                    |                                                                                                                                                
cTTGGCTGCCAGGATCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCCCGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGcc                                                                                                                                                 <  1:500419/150‑1 (MQ=255)
 ttGGCTGCCAGGATCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCa                                                                                                                                                >  2:5287/1‑150 (MQ=255)
       gCCAGGATCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGcc                                                                                                                                          >  2:907724/1‑150 (MQ=255)
        ccAGGTGCCGTTCGATTTCCTGCAGCGGGCCAGGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTGGGCCACCCGCCt                                                                                                                                         <  2:444293/150‑1 (MQ=255)
         cAGGATCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTg                                                                                                                                        >  2:295059/1‑150 (MQ=255)
          aGGATCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGa                                                                                                                                       >  1:1127880/1‑150 (MQ=255)
           ggATCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGAt                                                                                                                                      >  2:921865/1‑150 (MQ=255)
            gATCCGTTCGATTTCCCGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATc                                                                                                                                     <  2:566621/150‑1 (MQ=255)
              tCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGc                                                                                                                                   <  1:921865/150‑1 (MQ=255)
              tCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGANCGc                                                                                                                                   <  2:699324/150‑1 (MQ=255)
                 gTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGa                                                                                                                                >  1:978137/1‑150 (MQ=255)
                   tCGATTTCCTGCAGGTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAAc                                                                                                                              <  2:117180/150‑1 (MQ=255)
                   tCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAAc                                                                                                                              <  1:395985/150‑1 (MQ=255)
                     gATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACca                                                                                                                            >  2:364833/1‑150 (MQ=255)
                        ttCCTGCATCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCCCCGCACCTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAg                                                                                                                         <  2:847690/150‑1 (MQ=255)
                           cTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGcc                                                                                                                      >  2:1043276/1‑150 (MQ=255)
                                 cTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCggcggc                                                                                                                <  1:295059/150‑1 (MQ=255)
                                      cATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCcg                                                                                                                     <  1:628211/140‑1 (MQ=255)
                                      cATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCcg                                                                                                                     >  2:628211/1‑140 (MQ=255)
                                                   cAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCaaa                                                                                              <  1:364833/150‑1 (MQ=255)
                                                            gACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTg                                                                                     >  2:213769/1‑150 (MQ=255)
                                                               aTGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGc                                                                                        >  2:173605/1‑144 (MQ=255)
                                                               aTGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGc                                                                                        <  1:173605/144‑1 (MQ=255)
                                                                 ggctggcCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACgg                                                                                <  2:479130/150‑1 (MQ=255)
                                                                 ggctggcCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACgg                                                                                <  1:1043276/150‑1 (MQ=255)
                                                                          cGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGt                                                                       <  2:812489/150‑1 (MQ=255)
                                                                              gggCAGCTGGTCGATGAAGCGGCACACAAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAg                                                                   <  2:456282/148‑1 (MQ=255)
                                                                               tGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAg                                                                                                       <  2:642916/113‑1 (MQ=255)
                                                                               tGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAg                                                                                                       >  1:642916/1‑113 (MQ=255)
                                                                                     gggTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCt                                                            <  1:907724/150‑1 (MQ=255)
                                                                                        tCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGAc                                                         >  2:294711/1‑150 (MQ=255)
                                                                                                      caccagcaccagTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAAt                                           <  1:213769/150‑1 (MQ=255)
                                                                                                      caccagcaccagTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAAt                                           >  1:1081004/1‑150 (MQ=255)
                                                                                                       accagcaccagTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGTCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATg                                          >  2:123656/1‑150 (MQ=255)
                                                                                                       accagcaccagTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATg                                          >  1:862824/1‑150 (MQ=255)
                                                                                                         cagcaccagTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGGTGACAGCGAAGCACGAATGAt                                        >  1:730610/1‑150 (MQ=255)
                                                                                                          agcaccagTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATc                                       <  2:978137/150‑1 (MQ=255)
                                                                                                           gcaccagTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCa                                      <  2:848149/150‑1 (MQ=255)
                                                                                                            caccagTGCCACGATCACCGCAAGTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAg                                     >  2:553164/1‑150 (MQ=255)
                                                                                                              ccagTGCCACGATCACCGCAACTGCGGCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGc                                   >  1:151049/1‑150 (MQ=255)
                                                                                                                 gTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGTCACCCGCCTGATCGCCGAACCACAGGc                                                                                                                       <  2:363245/63‑1 (MQ=255)
                                                                                                                 gTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGTCACCCGCCTGATCGCCGAACCACAGGc                                                                                                                       >  1:929654/1‑63 (MQ=255)
                                                                                                                 gTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGTCACCCGCCTGATCGCCGAACCACAGGc                                                                                                                       >  1:363245/1‑63 (MQ=255)
                                                                                                                 gTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGTCACCCGCCTGATCGCCGAACCACAGGc                                                                                                                       <  2:929654/63‑1 (MQ=255)
                                                                                                                            aCCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGCCCATGCCGctgctg                     >  2:300783/1‑150 (MQ=255)
                                                                                                                            aCCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGCCCATGCCGctgctg                     >  1:417977/1‑150 (MQ=255)
                                                                                                                             ccGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGCCCATGCCGCtgctgc                    >  2:687733/1‑150 (MQ=255)
                                                                                                                                       gTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGccc                                 <  2:157878/127‑1 (MQ=255)
                                                                                                                                       gTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGccc                                 >  1:157878/1‑127 (MQ=255)
                                                                                                                                        tCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGCCCATGCCGCTGCTGCGCTGGCGCTcc         >  2:390851/1‑150 (MQ=255)
                                                                                                                                               cTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGCCCATGCCGCTGCTGCGCTGGCGCTCCAGGCttt  <  2:1081004/150‑1 (MQ=255)
                                                                                                                                               cTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGCCCATGCCGCTGCTGCGCTGGCGCTCCAGGCttt  <  2:862824/150‑1 (MQ=255)
                                                                                                                                               cTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGCCCAGAACTCTGCTGCGCTGGCGCTCCAGGCttt  >  1:840913/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CTTGGCTGCCAGGATCCGTTCGATTTCCTGCAGCTGGCCATGGGCCCCGCGCAGGATCTCGACATGGCTGGCCACGTCCTGCAGCGGGTCGATGAAGCGGCACACCAGCACCAGTGCCACGATCACCGCAACTGCGTCCTGCGCTTGGCCACCCGCCTGATCGCCGAACCACAGGCCGGCGGCCACCAACAGCGCGGCAAATGTTGCCTGCACGGCCCAACTGTTGAGCAGTGCTGACAGCGAAGCACGAATGATCAGGCCCATGCCGCTGCTGCGCTGGCGCTCCAGGCTTT  >  NC_002947/2965719‑2966011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑