Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,031,138 C→G 10.0% I134M (ATC→ATG)  mgtA → ATP‑dependent magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,031,1380CG10.0% 151.7 / 8.3 50I134M (ATC→ATG) mgtAATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base G (3/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

GCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGT  >  NC_002947/3031000‑3031287
                                                                                                                                          |                                                                                                                                                     
gCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCATGGTCATGATGATGATGACCTTGGt                                                                                                                                            <  1:1047974/150‑1 (MQ=255)
   aCTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCATGGTCATGATGATGATGACCTTGGTCAg                                                                                                                                         <  2:169620/150‑1 (MQ=255)
      gCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCt                                                                                                                                      <  2:613550/150‑1 (MQ=255)
        tCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                    >  1:788328/1‑150 (MQ=255)
              cccTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGcc                                                                                                                              >  2:200610/1‑150 (MQ=255)
                           gTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCCTGGTCAGCCTCAGCAGCCTGCTGCGATTCTg                                                                                                                 <  2:910521/150‑1 (MQ=255)
                               aTCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAg                                                                                                             <  1:392071/150‑1 (MQ=255)
                                    tGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAGGCAGACGACGCCGAGCTGAGCGCGCACATCATGATCATGACCACGGGCAGCCTCAGCGGGCAGGTGCCAGTGTGGCACGAggt                                                                                                        >  2:584465/1‑148 (MQ=255)
                                    tGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTa                                                                                                        >  2:529203/1‑150 (MQ=255)
                                     gTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTAc                                                                                                       <  2:1029054/150‑1 (MQ=255)
                                      tACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTAcc                                                                                                      <  1:1120942/150‑1 (MQ=255)
                                       aCTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCg                                                                                                     <  1:792345/150‑1 (MQ=255)
                                             gACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGaa                                                                                               <  1:529203/150‑1 (MQ=255)
                                                ccTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACaa                                                                                            <  1:833180/150‑1 (MQ=255)
                                                           tCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGAcg                                                                                 >  2:22651/1‑150 (MQ=255)
                                                              gCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                    <  2:752212/96‑1 (MQ=255)
                                                              gCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                    >  1:752212/1‑96 (MQ=255)
                                                              gCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                              <  1:939764/150‑1 (MQ=255)
                                                              gCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGAGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                              <  1:899972/150‑1 (MQ=255)
                                                               cTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCt                                                                             >  1:717540/1‑150 (MQ=255)
                                                                    tCACCGACTACTGGCTGCCGGTCAGGGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAg                                                                        >  1:704907/1‑150 (MQ=255)
                                                                    tCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTc                                                                                                  >  1:518193/1‑124 (MQ=255)
                                                                    tCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTc                                                                                                  <  2:518193/124‑1 (MQ=255)
                                                                      aCCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                              >  2:205479/1‑142 (MQ=255)
                                                                      aCCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                              <  1:205479/142‑1 (MQ=255)
                                                                       ccGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCCCAAAGcca                                                                     >  2:78219/1‑148 (MQ=255)
                                                                        cGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAAt                                                                    >  1:795454/1‑150 (MQ=255)
                                                                               tGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTgcgc                                                             >  1:254720/1‑150 (MQ=255)
                                                                                 gCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                    <  2:918147/77‑1 (MQ=255)
                                                                                 gCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                    >  1:918147/1‑77 (MQ=255)
                                                                                    gCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAgg                                                                                          <  2:425126/116‑1 (MQ=255)
                                                                                    gCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAgg                                                                                          >  1:425126/1‑116 (MQ=255)
                                                                                           aGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACg                                                 <  1:836007/150‑1 (MQ=255)
                                                                                                aGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGtt                                            <  2:216021/150‑1 (MQ=255)
                                                                                                  gCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTgc                                          >  2:954778/1‑150 (MQ=255)
                                                                                                                 ccGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGGTGCCGACGCACTCAAAGCAATGCTGCGCACTACGGCCACGGTGTTGCGCCACGCGCAGATcc                           >  2:261579/1‑149 (MQ=255)
                                                                                                                   gACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCggg                         <  1:291496/150‑1 (MQ=255)
                                                                                                                   gACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCggg                         <  1:44238/150‑1 (MQ=255)
                                                                                                                    aCCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGc                        <  1:386750/150‑1 (MQ=255)
                                                                                                                     gcTGACCATGGTCATGATGATGATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCa                       >  2:929894/2‑150 (MQ=255)
                                                                                                                     ccTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCa                       >  1:1019231/1‑150 (MQ=255)
                                                                                                                       tGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAg                     <  1:200610/150‑1 (MQ=255)
                                                                                                                         aCCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCa                                                            <  1:752236/109‑1 (MQ=255)
                                                                                                                         aCCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCa                                                            >  2:752236/1‑109 (MQ=255)
                                                                                                                          ccATGGTCATGATGATGATGACCTTGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCGCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGGGAGCAGATCGGGCAAGccc                  >  1:373873/1‑150 (MQ=255)
                                                                                                                          ccAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGccc                  >  2:6541/1‑150 (MQ=255)
                                                                                                                           catggTCATGATGATGATGACCTGGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGcccc                 >  2:840966/4‑150 (MQ=255)
                                                                                                                            aaGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCggg                         <  2:1067317/141‑1 (MQ=255)
                                                                                                                            aaGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCggg                         >  1:1067317/1‑141 (MQ=255)
                                                                                                                            aaGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCcg                >  1:1051810/1‑150 (MQ=255)
                                                                                                                                tcatcatcatcaTGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGTGCCCTGCTCGAACAAGACTGCCTACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGcc            >  1:690342/1‑150 (MQ=255)
                                                                                                                                   tcatcatcatGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTgc         <  1:839778/150‑1 (MQ=255)
                                                                                                                                        atcatGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAg                                                                        >  1:493474/1‑82 (MQ=255)
                                                                                                                                        atcatGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAg                                                                        <  2:493474/82‑1 (MQ=255)
                                                                                                                                          catGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGt  <  2:1019231/150‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                     
GCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGT  >  NC_002947/3031000‑3031287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑