Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,031,145 A→T 6.9% M137L (ATG→TTG)  mgtA → ATP‑dependent magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,031,1450AT 6.9% 180.2 / 4.3 59M137L (ATG→TTG) mgtAATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base T (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

GCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGTGCCCA  >  NC_002947/3031000‑3031292
                                                                                                                                                 |                                                                                                                                                   
gCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCATGGTCATGATGATGATGACCTTGGt                                                                                                                                                 <  1:1047974/150‑1 (MQ=255)
   aCTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCATGGTCATGATGATGATGACCTTGGTCAg                                                                                                                                              <  2:169620/150‑1 (MQ=255)
      gCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCt                                                                                                                                           <  2:613550/150‑1 (MQ=255)
        tCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                         >  1:788328/1‑150 (MQ=255)
              cccTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGcc                                                                                                                                   >  2:200610/1‑150 (MQ=255)
                           gTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCCTGGTCAGCCTCAGCAGCCTGCTGCGATTCTg                                                                                                                      <  2:910521/150‑1 (MQ=255)
                               aTCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAg                                                                                                                  <  1:392071/150‑1 (MQ=255)
                                    tGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAGGCAGACGACGCCGAGCTGAGCGCGCACATCATGATCATGACCACGGGCAGCCTCAGCGGGCAGGTGCCAGTGTGGCACGAggt                                                                                                             >  2:584465/1‑148 (MQ=255)
                                    tGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTa                                                                                                             >  2:529203/1‑150 (MQ=255)
                                     gTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTAc                                                                                                            <  2:1029054/150‑1 (MQ=255)
                                      tACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTAcc                                                                                                           <  1:1120942/150‑1 (MQ=255)
                                       aCTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCg                                                                                                          <  1:792345/150‑1 (MQ=255)
                                             gACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGaa                                                                                                    <  1:529203/150‑1 (MQ=255)
                                                ccTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACaa                                                                                                 <  1:833180/150‑1 (MQ=255)
                                                           tCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGAcg                                                                                      >  2:22651/1‑150 (MQ=255)
                                                              gCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                         <  2:752212/96‑1 (MQ=255)
                                                              gCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                         >  1:752212/1‑96 (MQ=255)
                                                              gCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                                   <  1:939764/150‑1 (MQ=255)
                                                              gCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGAGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                                   <  1:899972/150‑1 (MQ=255)
                                                               cTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCt                                                                                  >  1:717540/1‑150 (MQ=255)
                                                                    tCACCGACTACTGGCTGCCGGTCAGGGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAg                                                                             >  1:704907/1‑150 (MQ=255)
                                                                    tCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTc                                                                                                       >  1:518193/1‑124 (MQ=255)
                                                                    tCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTc                                                                                                       <  2:518193/124‑1 (MQ=255)
                                                                      aCCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                                   <  1:205479/142‑1 (MQ=255)
                                                                      aCCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACgcgc                                                                                   >  2:205479/1‑142 (MQ=255)
                                                                       ccGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCCCAAAGcca                                                                          >  2:78219/1‑148 (MQ=255)
                                                                        cGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAAt                                                                         >  1:795454/1‑150 (MQ=255)
                                                                               tGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTgcgc                                                                  >  1:254720/1‑150 (MQ=255)
                                                                                 gCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                         <  2:918147/77‑1 (MQ=255)
                                                                                 gCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTca                                                                                                                                         >  1:918147/1‑77 (MQ=255)
                                                                                    gCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAgg                                                                                               <  2:425126/116‑1 (MQ=255)
                                                                                    gCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAgg                                                                                               >  1:425126/1‑116 (MQ=255)
                                                                                           aGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACg                                                      <  1:836007/150‑1 (MQ=255)
                                                                                                aGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGtt                                                 <  2:216021/150‑1 (MQ=255)
                                                                                                  gCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTgc                                               >  2:954778/1‑150 (MQ=255)
                                                                                                                 ccGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGGTGCCGACGCACTCAAAGCAATGCTGCGCACTACGGCCACGGTGTTGCGCCACGCGCAGATcc                                >  2:261579/1‑149 (MQ=255)
                                                                                                                   gACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCggg                              <  1:44238/150‑1 (MQ=255)
                                                                                                                   gACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCggg                              <  1:291496/150‑1 (MQ=255)
                                                                                                                    aCCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGc                             <  1:386750/150‑1 (MQ=255)
                                                                                                                     gcTGACCATGGTCATGATGATGATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCa                            >  2:929894/2‑150 (MQ=255)
                                                                                                                     ccTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCa                            >  1:1019231/1‑150 (MQ=255)
                                                                                                                       tGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAg                          <  1:200610/150‑1 (MQ=255)
                                                                                                                         aCCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCa                                                                 <  1:752236/109‑1 (MQ=255)
                                                                                                                         aCCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCa                                                                 >  2:752236/1‑109 (MQ=255)
                                                                                                                          ccATGGTCATGATGATGATGACCTTGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCGCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGGGAGCAGATCGGGCAAGccc                       >  1:373873/1‑150 (MQ=255)
                                                                                                                          ccAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGccc                       >  2:6541/1‑150 (MQ=255)
                                                                                                                           catggTCATGATGATGATGACCTGGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGcccc                      >  2:840966/4‑150 (MQ=255)
                                                                                                                            aaGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCggg                              >  1:1067317/1‑141 (MQ=255)
                                                                                                                            aaGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCggg                              <  2:1067317/141‑1 (MQ=255)
                                                                                                                            aaGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCcg                     >  1:1051810/1‑150 (MQ=255)
                                                                                                                                tcatcatcatcaTGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGTGCCCTGCTCGAACAAGACTGCCTACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGcc                 >  1:690342/1‑150 (MQ=255)
                                                                                                                                   tcatcatcatGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTgc              <  1:839778/150‑1 (MQ=255)
                                                                                                                                        atcatGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAg                                                                             >  1:493474/1‑82 (MQ=255)
                                                                                                                                        atcatGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAg                                                                             <  2:493474/82‑1 (MQ=255)
                                                                                                                                          catGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGt       <  2:1019231/150‑1 (MQ=255)
                                                                                                                                           atGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGTg      >  1:941282/1‑150 (MQ=255)
                                                                                                                                            tGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGCCAGCGGGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGTGc     >  1:966039/1‑150 (MQ=255)
                                                                                                                                               ccATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGTGCCCa  <  1:6541/150‑1 (MQ=255)
                                                                                                                                               ccATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGTGCCCa  <  1:929894/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTCGAACAAGGCTGCCGACGCGCTCAAAGCAATGGTGCGCACTACGGCCACGGTGTTGCGCCGCGAGCAGATCGGGCAAGCCCCGCGCCTGCGCGAAGTGCCCA  >  NC_002947/3031000‑3031292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑