Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 308,450 C→A 7.1% intergenic (+1464/‑406) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947308,4500CA 7.1% 173.1 / 2.6 56intergenic (+1464/‑406)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (24/28);  new base A (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGCCGAGCTGCTGATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAACCGAACTGCTCAACCCGCGCACCAACTGGGCTGA  >  NC_002947/308307‑308598
                                                                                                                                               |                                                                                                                                                    
tCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGCCGAGCTGCTGATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCccacca                                                                                                                                                <  2:748554/150‑1 (MQ=255)
              ttcCCGCGTCCGGCTGGCGAGTACGCCGAGCTGCTGATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATc                                                                                                                                  >  1:1049924/1‑150 (MQ=255)
                        cGGCTGGCGAGTACGCCGAGCTGCTGATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATcc                                                                                                                        >  2:964286/1‑150 (MQ=255)
                         ggCTGGCGAGTACGCCGAGCTGCTGATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCa                                                                                                                       <  2:940682/150‑1 (MQ=255)
                          gCTGGCGAGTACGCCGAGCTGCTGATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCag                                                                                                                      >  2:990277/1‑150 (MQ=255)
                            tGGCGAGTACGCCGAGCTGCTGATCAGGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGACTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGACGTGATCGCTGCAATCCagag                                                                                                                    >  2:562588/1‑150 (MQ=255)
                             ggCGAGTACGCCGAGCTGCTGATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAgc                                                                                                                   >  2:62164/1‑150 (MQ=255)
                                    aCGCCGAGCTGCTGATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCAc                                                                                                            <  2:252895/150‑1 (MQ=255)
                                              gctgATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCCACCTCTCCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGc                                                                                                  <  1:631551/150‑1 (MQ=255)
                                                     aaGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAAcc                                                                                           >  2:594649/1‑150 (MQ=255)
                                                        cGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAg                                                                                        <  2:330812/150‑1 (MQ=255)
                                                          tATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCa                                                                                      >  2:329601/1‑150 (MQ=255)
                                                              aaCGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTg                                                                                  >  1:868564/1‑150 (MQ=255)
                                                               aCGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGa                                                                                         >  2:757022/1‑142 (MQ=255)
                                                               aCGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGa                                                                                         <  1:757022/142‑1 (MQ=255)
                                                               aCGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGa                                                                                         <  1:756963/142‑1 (MQ=255)
                                                               aCGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGa                                                                                         >  2:756963/1‑142 (MQ=255)
                                                                 ggCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAAc                                                                                            <  1:650995/137‑1 (MQ=255)
                                                                 ggCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAAc                                                                                            >  2:650995/1‑137 (MQ=255)
                                                                    ttCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGAcatc                                                                            >  1:39960/1‑150 (MQ=255)
                                                                     tCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGAcatca                                                                           <  1:1002582/150‑1 (MQ=255)
                                                                     tCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGAGCTCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGAAcatca                                                                           <  2:460408/150‑1 (MQ=255)
                                                                      cAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCAATGGTCGGTGCCGAAACCGAGCACCTGGAcatcat                                                                          <  2:1049924/150‑1 (MQ=255)
                                                                         cTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCCACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCTAAACCGAGCACCTGGACATCATCaa                                                                       >  2:181244/1‑150 (MQ=255)
                                                                          tCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTTGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAATCCGAGCACCTGGACATCATCAAc                                                                      <  2:13268/150‑1 (MQ=255)
                                                                          tCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACccgtgcc                                                                                                                                        >  2:51201/1‑84 (MQ=255)
                                                                          tCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACccgtgcc                                                                                                                                        <  1:51201/84‑1 (MQ=255)
                                                                            cAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCt                                                                    <  2:39960/150‑1 (MQ=255)
                                                                             aaGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTg                                                                   >  2:497227/1‑150 (MQ=255)
                                                                              aGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTgg                                                                  <  1:181244/150‑1 (MQ=255)
                                                                                gTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGAc                                                                <  1:1043359/150‑1 (MQ=255)
                                                                                gTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGAc                                                                >  2:1043735/1‑150 (MQ=255)
                                                                                  cTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGt                                                              <  1:413336/150‑1 (MQ=255)
                                                                                    aCCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGc                                                            <  2:689657/150‑1 (MQ=255)
                                                                                    aCCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGc                                                            <  1:594649/150‑1 (MQ=255)
                                                                                        ggTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGaa                                                        <  1:362042/150‑1 (MQ=255)
                                                                                             acacCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCACCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGaaa                                                                                             >  2:310884/1‑108 (MQ=255)
                                                                                             acacCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCACCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGaaa                                                                                             <  1:310684/108‑1 (MQ=255)
                                                                                             acacCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCACCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGaaa                                                                                             <  1:310884/108‑1 (MQ=255)
                                                                                             acacCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCACCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGaaa                                                                                             >  2:310684/1‑108 (MQ=255)
                                                                                                 cGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTcc                                               >  2:865417/1‑150 (MQ=255)
                                                                                                  ggCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCg                                              <  2:235449/150‑1 (MQ=255)
                                                                                                     cggACCGGTTGTTTCTACGGCGGTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGc                                           <  2:905041/149‑1 (MQ=255)
                                                                                                       gACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGt                                         <  1:329601/150‑1 (MQ=255)
                                                                                                            gtggtgGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGaaa                                    >  2:760196/1‑150 (MQ=255)
                                                                                                             tggtggCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCCCCCGTGCCGTGATCGCTGTAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAAc                                   <  1:562588/150‑1 (MQ=255)
                                                                                                                  gCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGc                                           <  2:522677/137‑1 (MQ=255)
                                                                                                                  gCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGc                                           >  1:522677/1‑137 (MQ=255)
                                                                                                                    tACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGc                                           >  2:229555/1‑135 (MQ=255)
                                                                                                                    tACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGc                                           <  1:229555/135‑1 (MQ=255)
                                                                                                                      cGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGTGCACCTGGACATAATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAACCGAACTGCt                          <  2:616121/150‑1 (MQ=255)
                                                                                                                          gTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAACCGAACTGCTCAAc                      <  1:1043735/150‑1 (MQ=255)
                                                                                                                               cAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCTAGCATCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAACCGAACTGCTCAACCcgcg                 >  2:317478/1‑150 (MQ=255)
                                                                                                                                  gcgcTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAACCGAACTGCTCAACCCGCGCAc              >  1:549567/1‑150 (MQ=255)
                                                                                                                                          gCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAACCGAACTGCTCAACCCGCGCACCAACTggg      >  1:580618/1‑150 (MQ=255)
                                                                                                                                           cATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAACCGAACTGCTCAACCCGCGCACCAACTGGGc     >  1:362715/1‑150 (MQ=255)
                                                                                                                                              ccccACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAACCGAACTGCTCAACCCGCGCACCAACTGGGCTGa  >  2:94974/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TCGGCGCACCGTTCTTCCCGCGTCCGGCTGGCGAGTACGCCGAGCTGCTGATCAAGCGTATCAACGGCTTCAACTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGTGGTGGCTACGGCGTTGGCAAGCGCTTCAGCATCCCCACCACCCGTGCCGTGATCGCTGCAATCCAGAGCGGCGCACTGGTCGGTGCCGAAACCGAGCACCTGGACATCATCAACCTGGACGTGCCGAAGGCCGTTCCGGGCGTTGAAACCGAACTGCTCAACCCGCGCACCAACTGGGCTGA  >  NC_002947/308307‑308598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑