Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,257,523 G→C 11.4% A158G (GCC→GGC)  PP_2854 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,257,5230GC11.4% 129.4 / 7.4 45A158G (GCC→GGC) PP_2854hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (17/22);  new base C (2/3);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TCTGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGTTGC  >  NC_002947/3257383‑3257667
                                                                                                                                            |                                                                                                                                                
tCTGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGTTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTGCCCCGACCAGGGTCTGGCCGCCGGCCAgac                                                                                                                                         <  1:510110/150‑3 (MQ=255)
 cTGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTaa                                                                                                                                        <  1:714900/150‑2 (MQ=255)
 cTGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTAc                                                                                                                                        <  1:737641/150‑1 (MQ=255)
 cTGCAGCACGCCCCCTTGGGCCAGGCGTTGCACGCTGCTGCCCTTGTTGTTGCTGTTATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTAc                                                                                                                                        <  1:126469/150‑1 (MQ=255)
  tGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCTGCCAGTAcc                                                                                                                                       <  1:471403/150‑1 (MQ=255)
   gcagcaGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCt                                                                                                                                      <  2:938016/150‑1 (MQ=255)
    cagcagGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGCCGGCCAgacc                                                                                                                                        >  2:652322/1‑144 (MQ=255)
    cagcagGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGCCGGCCAgacc                                                                                                                                        >  2:652222/1‑144 (MQ=255)
    cagcagGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGCCGGCCAgacc                                                                                                                                        <  1:652322/147‑4 (MQ=255)
    cagcagGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGCCGGCCAgacc                                                                                                                                        <  1:652222/147‑4 (MQ=255)
               cTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCtgtt                                                                                                                          <  1:788374/150‑1 (MQ=255)
                      cAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTAcggc                                                                                                                   >  1:683291/1‑150 (MQ=255)
                       aGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTAcggcg                                                                                                                  <  2:377285/150‑1 (MQ=255)
                        ggCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTAcggcgg                                                                                                                 <  2:935135/150‑1 (MQ=255)
                                                     cTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGata                                                                                    >  2:219872/1‑150 (MQ=255)
                                                              aTGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACgttg                                                                           <  1:718223/150‑1 (MQ=255)
                                                                          acacccacaccGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTAcgg                                                                                                                    <  2:276706/97‑1 (MQ=255)
                                                                          acacccacaccGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTAcgg                                                                                                                    >  1:276706/1‑97 (MQ=255)
                                                                            acccacaccGATGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCgtt                                                             >  1:321910/1‑150 (MQ=255)
                                                                               cacaccGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTg                                                                                                >  2:287428/1‑112 (MQ=255)
                                                                               cacaccGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTg                                                                                                <  1:287428/112‑1 (MQ=255)
                                                                               cacaccGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCgttgtt                                                          <  2:683291/150‑1 (MQ=255)
                                                                                acaccGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACgttg                                                                           >  2:779910/1‑132 (MQ=255)
                                                                                acaccGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACgttg                                                                           <  1:779910/132‑1 (MQ=255)
                                                                                  accGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTtgttgtt                                                       >  1:841419/1‑149 (MQ=255)
                                                                                    cGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCAc                                                     >  2:997199/1‑150 (MQ=255)
                                                                                      cTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACgttgtt                                                                         >  1:855236/1‑128 (MQ=255)
                                                                                      cTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACgttgtt                                                                         <  2:855236/128‑1 (MQ=255)
                                                                                           aGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGc                                              >  1:1100440/1‑150 (MQ=255)
                                                                                               acacTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACgg                                          >  2:105736/1‑150 (MQ=255)
                                                                                                 acTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCgttgtt                                                          <  1:47968/132‑1 (MQ=255)
                                                                                                 acTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCgttgtt                                                          >  2:47968/1‑132 (MQ=255)
                                                                                                 acTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGac                                        >  1:146474/1‑150 (MQ=255)
                                                                                                  cTGAGCGCGCCGGCGCCATTTTCCCCGACACGGGTGTGATACAAGACCACTATCTGAGCATGATGTGGTACCGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTTTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGacc                                       >  1:21463/1‑150 (MQ=255)
                                                                                                         cgcCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTggg                                <  2:1100440/150‑1 (MQ=255)
                                                                                                              gcgcCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAg                                                                    >  2:265020/1‑109 (MQ=255)
                                                                                                              gcgcCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAg                                                                    <  1:265020/109‑1 (MQ=255)
                                                                                                                  cATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCtt                       >  1:950953/1‑150 (MQ=255)
                                                                                                                      ttCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCggt                   <  1:1061563/150‑1 (MQ=255)
                                                                                                                          ccGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCggtggtg               >  2:1006925/1‑150 (MQ=255)
                                                                                                                          ccGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCggtggtg               <  1:1000695/150‑1 (MQ=255)
                                                                                                                          ccGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTAGGCTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCggtggtg               <  1:58062/150‑1 (MQ=255)
                                                                                                                                     cTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTc                      <  1:458037/132‑1 (MQ=255)
                                                                                                                                     cTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTc                      >  2:458037/1‑132 (MQ=255)
                                                                                                                                       ggCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGTTgc  <  1:997199/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
TCTGCAGCAGGCCACCTTGGGCCAGGCGTTGCACGCTGCTGCCCTGGTTGTTGCTGGCATTGATGTTGAGCTGCACACCCACACCGCTGGAAGACACACTGAGCGCGCCGGCGCCATTTTCCCCGACCAGGGTCTGGCCGGCGGCCAGTACCTGGCCTTGCTGTTGTACGGCGGGTGCCTGGTTGGCTTTGGTCACGTTGATATCGACGTTGTTGTAAGCGGCGTTGTTGTCACCGGCTGCACGGACCACCTGGGTTACACCTTCGGTGGTGGTCAGGCCGTTGC  >  NC_002947/3257383‑3257667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑