Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,615,341 C→A 8.1% A174E (GCA→GAA)  PP_3186 → transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,615,3410CA 8.1% 152.5 / 2.7 49A174E (GCA→GAA) PP_3186transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (17/28);  new base A (2/2);  total (19/30)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CTGGAGCTGCATGTGGCACTGGCACCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGG  >  NC_002947/3615193‑3615490
                                                                                                                                                    |                                                                                                                                                     
ctgGAGCTGCATGTGGCACTGGCACCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCgca                                                                                                                                                      <  2:503201/150‑1 (MQ=255)
   gAGCTGCATGTGGCACTGGCACCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCgcagca                                                                                                                                                   >  1:129593/1‑150 (MQ=255)
     gCTGCATGTGGCACTGGCACCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCagcagc                                                                                                                                                 <  2:899194/150‑1 (MQ=255)
         cATGTGGCACTGGCACCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAgcgcgc                                                                                                                                             <  2:1120798/150‑1 (MQ=255)
               gcactggcacCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCAc                                                                                                                                       <  1:220988/150‑1 (MQ=255)
                  ctggcacCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTg                                                                                                                                    <  1:903606/150‑1 (MQ=255)
                     gcacCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGAt                                                                                                                                 <  1:114863/150‑1 (MQ=255)
                      cacCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATg                                                                                                                                >  2:65033/1‑150 (MQ=255)
                        ccTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGaa                                                                                                                              <  1:921050/150‑1 (MQ=255)
                         cTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAAc                                                                                                                             >  1:739645/1‑150 (MQ=255)
                            gCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGTATGAACTgg                                                                                                                          <  1:327231/150‑1 (MQ=255)
                                 aTCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCcgcg                                                                                                                     >  2:864994/1‑150 (MQ=255)
                                  tCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCcgcgc                                                                                                                    >  2:516395/1‑150 (MQ=255)
                                    ggCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCgcgcgc                                                                                                                  >  2:657922/1‑150 (MQ=255)
                                         tGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCAt                                                                                                             <  1:65033/150‑1 (MQ=255)
                                            cGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGAc                                                                                                          <  1:673415/150‑1 (MQ=255)
                                                 tCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGagc                                                                                                     >  2:994392/1‑150 (MQ=255)
                                                         aCCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTc                                                                                             <  1:432557/150‑1 (MQ=255)
                                                               gCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGaa                                                                                       <  2:467333/150‑1 (MQ=255)
                                                                  gAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTg                                                                                    >  2:1006803/1‑150 (MQ=255)
                                                                   aGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTgg                                                                                   <  2:897579/150‑1 (MQ=255)
                                                                   aGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTgg                                                                                   <  2:811383/150‑1 (MQ=255)
                                                                      gcATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAg                                                                                <  2:1082657/150‑1 (MQ=255)
                                                                        aTTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAggg                                                                              >  2:841499/1‑150 (MQ=255)
                                                                         ttGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGa                                                                             >  1:716952/1‑150 (MQ=255)
                                                                             cGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGAtttt                                                                         <  1:670628/150‑1 (MQ=255)
                                                                                cTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTgg                                                                      <  1:190037/150‑1 (MQ=255)
                                                                                 ttGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGc                                                                     <  1:864994/150‑1 (MQ=255)
                                                                                         gtattccTACCGCAAGTGTATTCGCGAGAATGCCTGGTAGGGCTACCACGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGACCTGGCCGCGCGCAGCATGACCGTGCAGCGGTTCGCCGAACTGGCAGGGATTTTTAGCCAGGCCTc                                                             <  2:529318/145‑1 (MQ=255)
                                                                                           aCCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCa                                                           >  1:512548/1‑150 (MQ=255)
                                                                                             cccTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGc                                                         >  2:604391/1‑150 (MQ=255)
                                                                                               cTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAgagc                                                       >  1:529318/1‑147 (MQ=255)
                                                                                                   cgcgAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAg                                                   >  2:801937/1‑150 (MQ=255)
                                                                                                      gAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCg                                                <  2:739645/150‑1 (MQ=255)
                                                                                                           gATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCa                                                                   >  2:355679/1‑126 (MQ=255)
                                                                                                           gATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCa                                                                   <  1:355679/126‑1 (MQ=255)
                                                                                                                  gAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGAAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCa                                                                                 >  1:508177/1‑105 (MQ=255)
                                                                                                                  gAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGAAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCa                                                                                 <  2:508173/105‑1 (MQ=255)
                                                                                                                  gAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGAAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCa                                                                                 <  2:508177/105‑1 (MQ=255)
                                                                                                                  gAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGAAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCa                                                                                 >  1:508173/1‑105 (MQ=255)
                                                                                                                     tATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATg                                 <  2:512548/150‑1 (MQ=255)
                                                                                                                      aTGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATgg                                <  1:682900/150‑1 (MQ=255)
                                                                                                                          cGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGtt                            <  1:1006803/150‑1 (MQ=255)
                                                                                                                           ggggAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGAc                                                                                                          >  2:607027/1‑71 (MQ=255)
                                                                                                                           ggggAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGAc                                                                                                          <  1:607027/71‑1 (MQ=255)
                                                                                                                                gggCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACgg                      <  1:1091427/150‑1 (MQ=255)
                                                                                                                                 ggCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGtt                            >  2:1063470/1‑143 (MQ=255)
                                                                                                                                 ggCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGtt                            <  1:1063470/143‑1 (MQ=255)
                                                                                                                                  gCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGGCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGTGa                    <  2:129593/150‑1 (MQ=255)
                                                                                                                                      ccAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGAttt                                           <  1:25755/123‑1 (MQ=255)
                                                                                                                                      ccAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGAttt                                           >  2:25755/1‑123 (MQ=255)
                                                                                                                                                    cagcagCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGTGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTgg  <  1:801937/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGGAGCTGCATGTGGCACTGGCACCTTGCGTGATCGGCTATGCCGAAATCGGCCGGACCCTGGCCGAGCGCATTGGCGACTTGAGCAATCACCCCTACCGCGAGTGGATTGGCGAGTATGCCGGGGAGGGCTACCAAGGGGTGGCCGCAGCAGCGCGCAAGCACCTGGATGAACTGGCCGCGCGCAGCATGACCGAGCAGCGGTTCGCCGAACTGGCAGGGATTTTTGGCCAGGCCTCCAGCCTGGAGGCGGATTTCTGGCAAATGGGGTTGGACGGGACCGTGTAAACCGCCGTGG  >  NC_002947/3615193‑3615490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑