| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,633,834 | C→G | 13.0% | R63R (CGC→CGG) | PP_3202 → | transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,633,834 | 0 | C | G | 13.0% | 145.6 / 10.9 | 52 | R63R (CGC→CGG) | PP_3202 | transporter |
| Reads supporting (aligned to +/- strand): ref base C (20/25); new base G (4/3); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCGACAGCGCGTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGACGCGGTA > NC_002947/3633685‑3633981 | ttCGACAGCGCGTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCcgcgc > 2:932393/1‑150 (MQ=255) gcgTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCGCGCGGGatcatctt < 2:1095742/150‑3 (MQ=255) tCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTAGAGAAGATGATCCCGCGCGATCATCCGTTCATCcac < 1:932393/150‑1 (MQ=255) gcGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCcac < 1:1076918/149‑1 (MQ=255) gctgctGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACaa > 1:71807/1‑150 (MQ=255) ctgGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCGCGCGGGatcatcttct > 2:309761/1‑133 (MQ=255) ctgGAACGTGCGCGCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCGCGCGGGatcatcttct < 1:309761/137‑5 (MQ=255) cAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGa > 2:312044/1‑150 (MQ=255) caccGTGTGCTGGTGTAGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTc < 2:569370/150‑1 (MQ=255) accGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCg < 2:71807/150‑1 (MQ=255) gtgtGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGTCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCg < 1:1085429/150‑1 (MQ=255) gCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGCCAGTTCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCt < 1:345884/150‑1 (MQ=255) ggTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTggg < 1:312044/150‑1 (MQ=255) gtgtTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAAAAAATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGc < 1:356283/150‑1 (MQ=255) gctgctGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAAcgc > 2:74960/1‑150 (MQ=255) ctgctgTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAAcgcg < 1:406170/150‑1 (MQ=255) gctgTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGt < 1:207214/150‑1 (MQ=255) ctgTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACCACTACCGGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTg < 1:83768/150‑1 (MQ=255) tgtgCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTgc < 1:74960/150‑1 (MQ=255) tGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTgg < 1:649139/105‑1 (MQ=255) tGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTgg > 2:649139/1‑105 (MQ=255) tGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACGGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCg > 1:918751/1‑150 (MQ=255) tGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCg > 2:1016732/1‑150 (MQ=255) gACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGc < 2:244489/150‑1 (MQ=255) cTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCaa > 1:167193/1‑150 (MQ=255) tCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACGTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCcgcgcg > 2:132093/1‑150 (MQ=255) tCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCcgcgcg > 1:1084602/1‑150 (MQ=255) tCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCcgcgcg > 1:718764/1‑150 (MQ=255) ggCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGt < 2:990572/150‑1 (MQ=255) gCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTa < 2:519817/150‑1 (MQ=255) tGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGc > 2:394519/1‑150 (MQ=255) ggCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTa > 1:645767/1‑150 (MQ=255) ggCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTa > 1:645776/1‑150 (MQ=255) gCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACATCTACCTTGAGCACCGCCAGGAACTCGCCGTCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTat < 1:160490/150‑2 (MQ=255) cTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCCACGCGGTGCGCATCGCCGTGGCTAATCCGCGCGGTAGCATCTACGa < 1:25439/150‑1 (MQ=255) tCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGAc > 1:658837/1‑150 (MQ=255) gcgcCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGCTCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGt < 2:616711/108‑1 (MQ=255) gcgcCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGt > 1:616711/1‑108 (MQ=255) aCCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTAc < 2:718764/150‑1 (MQ=255) tCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGc > 2:722311/1‑150 (MQ=255) cAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTgg > 1:127059/1‑80 (MQ=255) cAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTgg < 2:127059/80‑1 (MQ=255) cAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCcgc < 2:273580/113‑1 (MQ=255) cAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCcgc > 1:273580/1‑113 (MQ=255) ttCGAGAAAATGTTCCACCGCTAACATCTGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTgcag < 2:906151/150‑1 (MQ=255) agaagaTGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCagcagc < 1:1016732/150‑1 (MQ=255) acggatgatCGCGCGGGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCt > 2:775631/4‑150 (MQ=255) ggatgatCGCGCGGGGTCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTACGCATCGCCGTGGCCAATCCGCGCGGTAGCCTCTACGACAAGGACTACATGCACAGCCTGCGGCAGCTcc > 2:982033/2‑149 (MQ=255) ggatgatCGCGCGGGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCa > 1:1083958/2‑150 (MQ=255) gatgatCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGt < 2:488869/90‑1 (MQ=255) gatgatCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGt > 1:488869/1‑90 (MQ=255) atgatCGCGCGGGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAac < 1:775631/150‑1 (MQ=255) ccGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGAcgcg > 1:380075/1‑150 (MQ=255) cgcgATCATCCGTTCATCCACAACTACCTGGAGCACCCCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGACGCGGta < 1:850522/150‑1 (MQ=255) | TTCGACAGCGCGTCCGGCTCGCTGCTGGAACGTGCGCTCTTCAACCACCGTGTGCTGGTGTTGCTGCTGTGCCTGGCGGCGACCTTGCTGCTCGGCTGGCAGGCCTCGCGCCTGACCCTCAATGCCAGCTTCGAGAAGATGATCCCGCGCGATCATCCGTTCATCCACAACTACCTGGAGCACCGCCAGGAACTCGCCGGCCTGGGCAACGCGGTGCGCATCGCCGTGGCCAATCCGCGCGGTAGCATCTACGACAAGGACTACCTGCACAGCCTGCAGCAGCTCAACGACGCGGTA > NC_002947/3633685‑3633981 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |