| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,763,549 | C→T | 14.3% | intergenic (+26/+1649) | PP_3326 → / ← PP_3328 | hypothetical protein/ring‑cleaving dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,763,549 | 0 | C | T | 14.3% | 111.1 / 11.1 | 49 | intergenic (+26/+1649) | PP_3326/PP_3328 | hypothetical protein/ring‑cleaving dioxygenase |
| Reads supporting (aligned to +/- strand): ref base C (20/22); new base T (3/4); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
GCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCACTTGCAT > NC_002947/3763402‑3763694 | gCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACgg > 1:272245/1‑150 (MQ=255) aaTGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGcc > 1:115696/1‑150 (MQ=255) aaTGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGcc > 1:115711/1‑150 (MQ=255) gAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCGTCATGGCCGAg < 2:374667/150‑1 (MQ=255) aGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCGTCATGGCCGAGGt < 1:392524/150‑1 (MQ=255) gACTCAACATCATCGCGGCCGAGGCGACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAACA‑AGCTGATATAAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTAt < 1:68647/150‑1 (MQ=255) gACTCAACATCATCGCGGCCAAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTCTCGTTCCTGGGTAAGCCGCACCACCAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTAt < 2:278048/150‑1 (MQ=255) cAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCa < 1:269628/150‑1 (MQ=255) catcatCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGtt < 1:164932/150‑1 (MQ=255) atcatcGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAg > 1:1016143/1‑138 (MQ=255) atcatcGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAg < 2:1016143/138‑1 (MQ=255) caccgcgGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGa < 2:292232/147‑1 (MQ=255) atcGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAg > 1:502131/1‑150 (MQ=255) gcgGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCg > 1:627085/1‑150 (MQ=255) cccAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCg < 2:502131/150‑1 (MQ=255) cAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGa > 1:969530/1‑150 (MQ=255) tACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCTCAATCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAg < 1:561400/150‑1 (MQ=255) ccTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGc > 2:658647/1‑150 (MQ=255) tGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCt < 2:821601/150‑1 (MQ=255) tGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCt < 1:553931/150‑1 (MQ=255) aGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCa > 2:803170/1‑150 (MQ=255) aCGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGCCATACCTCGGCCATGACGGCCGAGGTATGGCAGTTTGAGGCGTTCGGAATGAGTGCCTTCAACCAGCATc < 2:852295/150‑1 (MQ=255) aCGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCt > 1:181378/1‑138 (MQ=255) aCGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCt < 2:181378/138‑1 (MQ=255) gCCCCGTGGCCCAGCAGAGCCTGT‑TCTGTTCATTTTCGTTCCTGGGTAAGCCGCAACACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCAcc < 2:9165/150‑1 (MQ=255) ccccGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGa > 1:1054812/1‑125 (MQ=255) ccccGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGa < 2:1054812/125‑1 (MQ=255) ccAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTg < 2:575716/150‑1 (MQ=255) cagAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGAATCCGCACCATGTCCATTGGcac < 2:272245/150‑1 (MQ=255) ccTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGcc > 2:360513/1‑150 (MQ=255) gttcaCTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCg < 2:969530/150‑1 (MQ=255) ttcaCTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCGTCATGGCCGAGGTAt > 2:1075763/1‑74 (MQ=37) ttcaCTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCGTCATGGCCGAGGTAt < 1:1075763/74‑1 (MQ=37) ttcaCTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCgg > 1:964773/1‑150 (MQ=255) cGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACgg < 1:803170/150‑1 (MQ=255) tCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCaa > 2:408509/1‑150 (MQ=255) tCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCaa < 2:627085/150‑1 (MQ=255) tAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTCTGACACTTGGAGGCGCAGCCTGTGGTTGTCTGGAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGCCAATCGAaca > 1:980910/1‑150 (MQ=255) gCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAATTCGAGGCGTTCAGTAACAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGa > 1:981295/1‑150 (MQ=255) gCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATgg > 2:448114/1‑150 (MQ=255) aTACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGTCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCatgacg < 1:360513/150‑3 (MQ=255) aCCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCca > 1:962886/1‑104 (MQ=255) aCCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCca < 2:962886/104‑1 (MQ=255) ccTCGGCCGTCATGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATgg > 2:801486/1‑124 (MQ=255) ccTCGGCCGTCATGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATgg < 1:801486/124‑1 (MQ=255) tCGGCCGTCATGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCAc > 1:611254/1‑150 (MQ=255) cGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCACt > 1:82200/1‑150 (MQ=255) ccATGACGGCCGAGGTATGGCAGTTGGAGGAGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGCTGGGCATGTCGATTACCTGCATGATGGGCACTTGc < 2:594387/150‑1 (MQ=255) aTGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCACTTGCAt > 2:140686/1‑150 (MQ=255) | GCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGT‑TCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGCACTTGCAT > NC_002947/3763402‑3763694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |