Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,872,661 T→A 14.7% intergenic (‑237/+71) PP_3420 ← / ← PP_3421 sensor histidine kinase/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,872,6610TA14.7% 75.5 / 8.2 34intergenic (‑237/+71)PP_3420/PP_3421sensor histidine kinase/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (13/16);  new base A (2/3);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01

CTGCCATTTGTCGCGTGTCGCGCTTCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAACGAATTCACGCCAGTCGGAAACTTAACGAAACCCTTGTGCCATGGCCTTCACTGCTGCTGAGCCGGAC  >  NC_002947/3872515‑3872794
                                                                                                                                                  |                                                                                                                                     
cTGCCATTTGTCGCGTGTCGCGCTTCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATc                                                                                                                                    <  1:1047436/150‑1 (MQ=255)
 tGCCATTTGTCGCGTGTCGCGCTTCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCa                                                                                                                                   <  2:780790/150‑1 (MQ=255)
  gCCATTTGTCGCGTGTCGCGCTTCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGTGATAATccc                                                                                                                                  >  2:606389/1‑148 (MQ=255)
   ccATTTGTCGCGTGTCGCGCTTCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCAcc                                                                                                                                 <  1:154875/150‑1 (MQ=255)
        tgtcgcgtgtcgcgCTTCACAAACTTCGCTGCAAAAACTTGGCAAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACACTTGGGTGATAATCCCCCaaat                                                                                                                            <  2:943861/150‑2 (MQ=255)
            gcgtgtcgcgCTTCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAaca                                                                                                                        >  1:531331/1‑150 (MQ=255)
              gtgtcgcgCTTCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGTGATAATCCCCCaaatgtgggg                                                                                                                      <  1:606389/150‑8 (MQ=255)
                 tcgcgCTTCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAAt                                                                                                                   <  2:1083340/150‑1 (MQ=255)
                        tCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTc                                                                                                            <  2:1031730/150‑1 (MQ=255)
                           caAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCata                                                                                                         >  1:272505/1‑150 (MQ=255)
                           caAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCata                                                                                                         >  1:766324/1‑150 (MQ=255)
                           caAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCata                                                                                                         <  1:839058/150‑1 (MQ=255)
                            aaaCTTCGCTGCAAAAACTTGGCCAGCAGAAACATTACAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCatat                                                                                                        <  2:523527/150‑1 (MQ=255)
                                    cTGCAAAAACTTGGCCAGCAGAAACATGCCAAATTAAAAATGTTATTCTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGACGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCt                                                                                                <  1:345152/150‑1 (MQ=255)
                                      gCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGTGATAATCCCCCaaatgtggg                                                                                                                       >  1:1077729/1‑119 (MQ=255)
                                      gCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGTGATAATCCCCCaaatgtggg                                                                                                                       <  2:1077729/125‑7 (MQ=255)
                                       cAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTAc                                                                                             >  2:531909/1‑150 (MQ=255)
                                          aaaCTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTa                                                                                              <  1:849282/146‑1 (MQ=255)
                                          aaaCTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTa                                                                                              >  2:849282/1‑146 (MQ=255)
                                           aaCTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGaa                                                                                         <  1:803853/150‑1 (MQ=255)
                                                   cagcagAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTg                                                                                 >  2:1128349/1‑150 (MQ=255)
                                                    agcagAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGa                                                                                <  2:272505/150‑1 (MQ=255)
                                                               tCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAAc                                                                     <  1:691446/150‑1 (MQ=255)
                                                                       aaaaaTGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAACGAATTCAc                                                             <  1:719806/150‑1 (MQ=255)
                                                                         aaaTGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAACGAATTCACGc                                                           >  1:960846/1‑150 (MQ=255)
                                                                         aaaTGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATGTAACGAATTCACGc                                                           >  1:109139/1‑150 (MQ=255)
                                                                              ttATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAGCCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAACGAATTCACGCCAGTc                                                      >  1:215519/1‑150 (MQ=255)
                                                                                             ttGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAACGAATTCACGCCAGTCGGAAACTTAACGaaa                                       <  1:1128349/150‑1 (MQ=255)
                                                                                                                   attaGAGGATTAACCCCCACATTGGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATGAACATCTGACTGAACAATCCTGATGTCATTTAACGAATTCACGCCAGTCGGAAACTTAACGAAACCCTTGTGCAATGGCCTTCAct                 >  2:618587/1‑150 (MQ=255)
                                                                                                                              aaCCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAACGAATTCACGCCAGTCGGAAACTTAACGAAACCCTTGTGCCATGGCCTTCACTGCTGCTGatcc      >  2:159001/1‑147 (MQ=255)
                                                                                                                               aCCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATc                                                                                    >  1:625201/1‑71 (MQ=255)
                                                                                                                               aCCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATc                                                                                    <  2:625201/71‑1 (MQ=255)
                                                                                                                               aCCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAACGAATTCACGCCAGTCGGAAACTTAACGAAACCCTTGTGCCATGGCCTTCACTGCTGCTGAGCCg     <  2:531331/150‑1 (MQ=255)
                                                                                                                                  cccACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAACGAATTCACGCCAGTCGGAAACTTAACGAAACCCTTGTGCCATGGCCTTCACTGCTGCTGAGCCGGAc  >  2:348672/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                     
CTGCCATTTGTCGCGTGTCGCGCTTCACAAACTTCGCTGCAAAAACTTGGCCAGCAGAAACATTCCAAATTAAAAATGTTATTTTTCAGTAGGTTGCCATAACTTATTAAGATTAATTAGAGGATTAACCCCCACATTTGGGGGATTATCACCCAAAAAACACAAATCCTTTTCATATCAACATCTTACTGAACAATCCTGATGTCATTTAACGAATTCACGCCAGTCGGAAACTTAACGAAACCCTTGTGCCATGGCCTTCACTGCTGCTGAGCCGGAC  >  NC_002947/3872515‑3872794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑