Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,921,245 C→G 6.1% E189Q (GAG→CAG)  acs ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,921,2450CG 6.1% 207.3 / 4.6 66E189Q (GAG→CAG) acsacyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (36/26);  new base G (2/2);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

CCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGC  >  NC_002947/3921098‑3921393
                                                                                                                                                   |                                                                                                                                                    
ccAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTcgc                                                                                                                                                    >  2:474074/1‑150 (MQ=255)
ccAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTcgc                                                                                                                                                    >  2:1112646/1‑150 (MQ=255)
          aCCGACCATGTACCCGGTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGTGAAACCGGTGGTGACTCAGGGGGACAGGATGTCGATCAGCGCGGCGCTACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACacc                                                                                                                                          >  2:262624/1‑148 (MQ=255)
           ccGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCATCTCCGTTGACAAcc                                                                                                                                         >  2:308019/1‑150 (MQ=255)
            cGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCt                                                                                                                                        <  1:1112646/150‑1 (MQ=255)
            cGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCt                                                                                                                                        <  1:718617/150‑1 (MQ=255)
              aCCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGGGCCGGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCg                                                                                                                                      <  2:588693/150‑1 (MQ=255)
               ccATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCgg                                                                                                                                     <  1:118417/150‑1 (MQ=255)
                cATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGc                                                                                                                                    >  1:925148/1‑150 (MQ=255)
                      cccGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAAGCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGGCAGGGCCTCGCGGCTGACAACCTCGGCACgggg                                                                                                                              >  2:333465/1‑149 (MQ=255)
                         gttgttGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACgg                                                                                                                                <  1:700900/145‑1 (MQ=255)
                         gttgttGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACgg                                                                                                                                >  2:700900/1‑145 (MQ=255)
                                aGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGGTCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCg                                                                                                                    <  2:185953/150‑1 (MQ=255)
                                 ggATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGt                                                                                                                   <  2:804500/150‑1 (MQ=255)
                                  gATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTg                                                                                                                  >  1:568390/1‑150 (MQ=255)
                                   atatTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTgc                                                                                                                 <  1:276779/150‑1 (MQ=255)
                                     cttGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTgcca                                                                                                               <  2:278303/149‑1 (MQ=255)
                                        gCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGACTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTgccaggc                                                                                                            <  2:688393/150‑1 (MQ=255)
                                              gCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGaaa                                                                                                      >  2:1060199/1‑150 (MQ=255)
                                               cTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAAc                                                                                                     >  2:206126/1‑150 (MQ=255)
                                                tAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAAcc                                                                                                    >  2:1011452/1‑150 (MQ=255)
                                                     gTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACgg                                                                                                                                >  2:78458/1‑117 (MQ=255)
                                                     gTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACgg                                                                                                                                <  1:78458/117‑1 (MQ=255)
                                                         cccccTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTg                                                                                           >  1:814423/1‑150 (MQ=255)
                                                            ccTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCg                                                                                        >  2:1080816/1‑150 (MQ=255)
                                                             cTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCGGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGa                                                                                       >  1:609656/1‑150 (MQ=255)
                                                              ttGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGc                                                                                         <  1:882525/147‑1 (MQ=255)
                                                              ttGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGc                                                                                         >  2:882525/1‑147 (MQ=255)
                                                                 gggAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAgc                                                                                   >  1:530857/1‑150 (MQ=255)
                                                                     aaCCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCa                                                                               <  2:530857/150‑1 (MQ=255)
                                                                       ccGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAgg                                                                             >  1:610020/1‑150 (MQ=255)
                                                                        cGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGc                                                                            >  1:224259/1‑150 (MQ=255)
                                                                         ggtggtGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGAc                                                                                                                                             <  2:317262/84‑1 (MQ=255)
                                                                         ggtggtGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGAc                                                                                                                                             >  1:317262/1‑84 (MQ=255)
                                                                                  tGAGGTGTACTGGATGTTGAGCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGAccacgcc                                                                  >  2:741614/1‑150 (MQ=255)
                                                                                   gAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGAccacgcca                                                                 <  1:1060199/150‑1 (MQ=255)
                                                                                           cTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTc                                                         >  1:459350/1‑150 (MQ=255)
                                                                                            tGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCg                                                        >  2:858741/1‑150 (MQ=255)
                                                                                             ggATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGc                                                                                         >  2:134699/1‑116 (MQ=255)
                                                                                             ggATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCgg                                                       <  1:1011452/150‑1 (MQ=255)
                                                                                             ggATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCCCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGc                                                                                         <  1:134699/116‑1 (MQ=255)
                                                                                               aTGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCgggg                                                     >  2:994839/1‑150 (MQ=255)
                                                                                                  ttGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAAc                                                  <  1:206126/150‑1 (MQ=255)
                                                                                                  ttGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAAc                                                  >  1:798621/1‑150 (MQ=255)
                                                                                                    gATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCg                                                >  1:998275/1‑150 (MQ=255)
                                                                                                      tCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCt                                              >  1:464109/1‑150 (MQ=255)
                                                                                                          gtcgtcGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCa                                          >  2:833811/1‑150 (MQ=255)
                                                                                                              tcgCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCcg                                                                                                 <  1:1062732/91‑1 (MQ=255)
                                                                                                              tcgCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCcg                                                                                                 >  2:1062732/1‑91 (MQ=255)
                                                                                                              tcgCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAg                                                                              <  2:304767/110‑1 (MQ=255)
                                                                                                              tcgCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAg                                                                              >  1:304767/1‑110 (MQ=255)
                                                                                                              tcgCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATc                                      >  2:418189/1‑150 (MQ=255)
                                                                                                                gCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAg                                    >  2:1046458/1‑150 (MQ=255)
                                                                                                                 cAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAgc                                   <  2:925148/150‑1 (MQ=255)
                                                                                                                           gtgCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGtt                         <  1:233711/150‑1 (MQ=255)
                                                                                                                               cTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGgct                     >  2:753542/1‑150 (MQ=255)
                                                                                                                                      gcggcgAGGCCCTGGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGtt                         >  1:297422/1‑139 (MQ=255)
                                                                                                                                      gcggcgAGGCCCTGGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGtt                         <  2:294612/139‑1 (MQ=255)
                                                                                                                                      gcggcgAGGCCCTGGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGtt                         <  2:297422/139‑1 (MQ=255)
                                                                                                                                      gcggcgAGGCCCTGGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGtt                         >  1:294612/1‑139 (MQ=255)
                                                                                                                                            aGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGccc        >  2:889093/1‑150 (MQ=255)
                                                                                                                                             gggCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCg       <  1:858741/150‑1 (MQ=255)
                                                                                                                                               gCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCggg     <  2:709974/150‑1 (MQ=255)
                                                                                                                                                ccTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTc                                             >  1:581412/1‑109 (MQ=255)
                                                                                                                                                ccTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTc                                             <  2:581412/109‑1 (MQ=255)
                                                                                                                                                ccTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGATGGCTGCTGGCCAGGCCCGGGa    >  2:558094/1‑150 (MQ=255)
                                                                                                                                                  tCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGc  >  1:1124134/1‑150 (MQ=255)
                                                                                                                                                  tCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGc  <  2:459350/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGC  >  NC_002947/3921098‑3921393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑