| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,091,493 | A→C | 7.8% | N20H (AAC→CAC) | garD → | galactarate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,091,493 | 0 | A | C | 7.8% | 130.3 / 2.3 | 50 | N20H (AAC→CAC) | garD | galactarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (27/19); new base C (2/2); total (29/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
AGCTCCCGTCACTGAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCAAAT > NC_002947/4091344‑4091641 | aGCTCCCGTCACTGAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACTTGCAGTTGATCGAACATTCCGACTAGAGACGCGACGTGC‑‑GCCTGCATGACGACGACa > 1:684324/1‑150 (MQ=255) tGAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGgtcg > 1:489272/1‑150 (MQ=255) gAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGgtcgg > 2:756617/1‑149 (MQ=255) gaaaaaCACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACCAAAACCCGAGAACCTGACAGGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTCCACGCCGACGACAACGCCGTGgtcgtc > 2:283248/2‑150 (MQ=255) aaaCACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACCACGACGTTgtcgtcgtc < 1:439447/150‑1 (MQ=255) cGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCtt < 2:391617/150‑1 (MQ=255) ggTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTgg < 2:571822/150‑1 (MQ=255) gTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAacga > 1:137024/1‑138 (MQ=255) gTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAacga < 2:137024/138‑1 (MQ=255) cccGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGaa < 2:26342/150‑1 (MQ=255) accGGAATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGaa < 1:566598/149‑1 (MQ=255) ggCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGgc > 2:785902/1‑150 (MQ=255) gCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCt > 1:1018599/1‑150 (MQ=255) gCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCt > 1:714257/1‑150 (MQ=255) gCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCt > 2:252941/1‑150 (MQ=255) gATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAGCGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCg < 1:643306/150‑1 (MQ=255) aTGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGc < 2:896014/150‑1 (MQ=255) aaCCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCtt > 2:236330/1‑112 (MQ=255) aaCCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCtt < 1:236330/112‑1 (MQ=255) cTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCg > 2:71646/1‑150 (MQ=255) tGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAacga > 2:998085/1‑99 (MQ=255) tGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAacga < 1:998085/99‑1 (MQ=255) aGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGATAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCg < 1:747757/150‑1 (MQ=255) tAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTg > 2:914435/1‑150 (MQ=255) tAGGGCCCAAACATGCAGTCGATCGAACATTCCGACTCGCCCCGCTACGTCCAAGCGTTTCTGAC‑‑TGATACCGTCGTGGTCGTCGTCAACAACGCCGGCTTGGGCGAAGGCGCAATCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTg < 1:252941/150‑1 (MQ=255) gggCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGcc < 2:847229/150‑1 (MQ=255) aaaCATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAg > 1:305602/1‑150 (MQ=255) aaaCATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAg < 1:709326/150‑1 (MQ=255) gTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGt > 2:272207/1‑150 (MQ=255) aTCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGcc > 2:540268/1‑150 (MQ=255) tCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCa > 1:174613/1‑150 (MQ=255) gAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCAcc > 1:173347/1‑150 (MQ=255) gAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGCCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAAGTGGCCAcc > 1:1011768/1‑150 (MQ=255) aaCATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAg > 1:724927/1‑84 (MQ=255) aaCATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAg < 2:724927/84‑1 (MQ=255) aTTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCgtgt > 1:154195/1‑150 (MQ=255) aTTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCgtgt < 2:226654/150‑1 (MQ=255) tagCCCCGCTACGTCC‑‑GCCTGCACGACGACGACCACGTCCTGGTCGGCGTCAACGCCGGCCGCTTGGGCGAACGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAACGCCATAAGGTGGCCACCGTGTCCATCGcc > 2:55613/3‑150 (MQ=255) tCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGcc > 1:901554/1‑150 (MQ=255) gCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATc < 1:493339/103‑1 (MQ=255) gCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATc > 2:493339/1‑103 (MQ=255) ccccGCTACGTCC‑‑GCCCGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCAGCGCCAAg > 2:569909/1‑150 (MQ=255) cccGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAgg > 1:51443/1‑150 (MQ=255) cccGATACGTCC‑‑GCCTGCACTACGACAACAACGTCTTTTTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCAGAAGGCATGACGCTCACCGCCAGCGGGCCTCAACGCCATAAGCAGGCCACCGTGTCCATCGCCAAgg > 2:514768/1‑150 (MQ=255) cTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGa < 2:1046634/150‑1 (MQ=255) cgacgacAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACgg < 1:756617/150‑1 (MQ=255) gacgacCACGACGTTGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGc > 1:292772/1‑88 (MQ=38) gacgacCACGACGTTGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGc < 2:292772/88‑1 (MQ=39) gacAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCaa > 1:596161/1‑150 (MQ=255) acAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCaaa < 1:71646/150‑1 (MQ=255) cAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCAAat > 1:656225/1‑150 (MQ=255) | AGCTCCCGTCACTGAAAAAACACGCCCGCGCCGGGTCGCCCGGCATGGGCGGATGTACAAAAGCCTGAGAACCTGACAAGTAGGGCCCAAACATGCAGTTGATCGAACATTCCGACTCGCCCCGCTACGTCC‑‑GCCTGCACGACGACGACAACGTCGTGGTCGTCGTCAACGACGGCGGCTTGGGCGAAGGCGCCCGCTTCGCCGACGGCCTGACCCTGATCGAAGGCGTGCCGCAAAGCCATAAGGTGGCCACCGTGTCCATCGCCAAGGGCGAGCCCGTGCGCCGCTACGGGCAAAT > NC_002947/4091344‑4091641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |