| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,176,896 | C→A | 9.9% | Y511* (TAC→TAA) | PP_3676 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,176,896 | 0 | C | A | 9.9% | 121.4 / 2.0 | 40 | Y511* (TAC→TAA) | PP_3676 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (21/15); new base A (2/2); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AAATTTGAGGCCGCTGGCATCACCCGCGGAGTGCTCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACAAAAGAGCAAATGCAGGACTTCA > NC_002947/4176747‑4177038 | aaaTTTGAGGCCGCTGGCATCACCCGCGGAGTGCTCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTAc < 1:930910/150‑1 (MQ=255) aTTTGAGGCAGCTGGCGTCACCCGCGGAGTGCTCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTACCCGACTTATTGGCAAATACATGTCAAGTTGTAGAGATTTGTTACAt > 2:345971/1‑150 (MQ=255) gCCGCTGGCATCACCCGCGGAGTGCTCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAg > 1:836911/1‑150 (MQ=255) gCTGGCATCACCCGCGGAGTGCTCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTgg < 1:19289/150‑1 (MQ=255) gcgGAGTGCTCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCt > 1:646426/1‑150 (MQ=255) ggAGTGCTCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTgg < 2:635390/150‑1 (MQ=255) cTCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACt > 1:664341/1‑150 (MQ=255) tCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTa > 2:688342/1‑150 (MQ=255) ccGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAg < 2:79254/150‑1 (MQ=255) aTTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGAATTCTTAGGAta > 2:922646/1‑150 (MQ=255) cTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTCTCAGACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATtctc < 1:922646/150‑1 (MQ=255) gcagcaTTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAAc < 1:453244/150‑1 (MQ=255) caTTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTg < 2:342755/150‑1 (MQ=255) caTTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTg < 1:349773/150‑1 (MQ=255) aTTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTg > 1:738908/1‑83 (MQ=255) aTTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTg < 2:738908/83‑1 (MQ=255) ttCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGCTTTGTTACATCCCTAAGTGGTAAAAATACCGCTGGGGCACTAGACTTCTTAGCATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGACCCTTGGc > 2:571190/1‑150 (MQ=255) ttCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGCAGGTGaaacctcga > 1:661714/1‑143 (MQ=255) aaaGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAg > 2:838420/1‑150 (MQ=255) aaaGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCACTGGTGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAg > 1:980001/1‑150 (MQ=255) agagTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTAtt < 2:101784/117‑1 (MQ=255) agagTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTAtt > 1:101784/1‑117 (MQ=255) tGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAgg < 1:505143/126‑1 (MQ=255) tGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAgg > 2:505143/1‑126 (MQ=255) ttACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGAc > 1:559552/1‑150 (MQ=255) tGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGaaa < 1:909145/95‑1 (MQ=255) tGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGaaa > 2:909145/1‑95 (MQ=255) tGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCa > 1:1122872/1‑150 (MQ=255) ggAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTAt < 2:33513/98‑1 (MQ=255) ggAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTAt > 1:33513/1‑98 (MQ=255) gAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTa < 2:3119/150‑1 (MQ=255) tACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACaaa > 2:959633/1‑150 (MQ=255) aTGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACaaaa > 2:725894/1‑148 (MQ=255) aTGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACaaaa < 1:725894/148‑1 (MQ=255) ccAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACAAAAGAGCa > 1:254512/1‑150 (MQ=255) cAAGTTGTAGAGATTTGTTAAATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACAAAAGAGCaa > 1:454763/1‑150 (MQ=255) cAAGTTGTAGAGATTTGTTAAATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACAAAAGAGCaa > 1:1028067/1‑150 (MQ=255) agaTTTGTTACATCCCTAAGTGGTTAAAATAACTCATGGTCTCTAGACTTCTTATGATCCTACAACAGAAATTCATTTATTCTGCTGGGCGTCGGT‑TACCTTGGCAAGTTGCGGCATCCGACTTCACTACAAAAGAGCAAATTCAGGACt > 1:1102277/1‑150 (MQ=255) aTTTGTTAAATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACAAAAGAGCAAATGCAGGACTTc < 2:454763/150‑1 (MQ=255) aTTTGTTAAATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACAAAAGAGCAAATGCAGGACTTc < 2:1028067/150‑1 (MQ=255) tttGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACAAAAGAGCAAATGCAGGACTTCa < 2:836911/150‑1 (MQ=255) | AAATTTGAGGCCGCTGGCATCACCCGCGGAGTGCTCACCCTATTCCGTATTCGATGCACACAGGGCATTCTAGAATTTTTACGGAACGGCAGCATTCAAAGAGTAGCTGACTTACTGGGAAATACATGCCAAGTTGTAGAGATTTGTTACATCCCTAAGTGGTTAAAATACCGCTGGGGCACTAGACTTCTTAGGATACTACAACAGAAATTTATTCTCGTAGC‑AACTGAAGGTGAACCTTGGCAAGTTGCGGCATCCGACTTCACTACAAAAGAGCAAATGCAGGACTTCA > NC_002947/4176747‑4177038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |