Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,301,102	A→G	8.4%	T75A (ACG→GCG)	PP_3774 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,301,102	0	A	G	8.4%	188.6 / 3.7	68	T75A (ACG→GCG)	PP_3774	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/26);  new base G (2/4);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACC  >  NC_002947/4300966‑4301251                                                                                                                                         |                                                                                                                                                      tGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAgcgc                                                                                                                                          >  2:199925/1‑150 (MQ=255)  gAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGcc                                                                                                                                         >  2:934403/1‑150 (MQ=255)  gAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGcc                                                                                                                                         >  2:934424/1‑150 (MQ=255)   aaGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGccc                                                                                                                                        >  2:82394/1‑150 (MQ=255)     gCGATTGCCCGTTTGCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAACAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAg                                                                                                                                      <  1:142505/150‑1 (MQ=255)     gCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAg                                                                                                                                      >  2:91209/1‑150 (MQ=255)      cGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAgg                                                                                                                                     <  2:162932/150‑1 (MQ=255)      cGATTGCCCGCTGTCCCAGTATCGTGACGCTGCTCAGCGATGGCTCTAGCGAGGTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAGGCACCCACAGGAGACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAgt                                                                                                                                     <  2:618716/150‑2 (MQ=255)         ttGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCGCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGagc                                                                                                                                  >  1:165266/1‑148 (MQ=255)            cccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                               >  1:157894/1‑150 (MQ=255)             ccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCt                                                                                                                                                   <  2:91786/129‑1 (MQ=255)             ccGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCt                                                                                                                                                   >  1:91786/1‑129 (MQ=255)             ccGCTTTCCCAGCATCGTGACGGTGCTAAGCGATGGCTCGAACCAGTTCTTAGCCCCAACCAAAGGTGCTTCTAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCaata                                                                                                                              >  2:280826/1‑147 (MQ=255)               gCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAgcgc                                                                                                                                          <  2:767404/136‑1 (MQ=255)               gCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAgcgc                                                                                                                                          >  1:767404/1‑136 (MQ=255)                cTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGaa                                                                                                                           <  1:866484/150‑1 (MQ=255)                  ttCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATc                                                                                                                         <  1:339096/150‑1 (MQ=255)                    cccAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGAGCCCAGGATCACTGGAATCGc                                                                                                                       >  2:287052/1‑150 (MQ=255)                     ccAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGCCTGGACGCTGGCCAGCCCCCAGGATCACTGGAATCGcc                                                                                                                      >  1:138730/1‑150 (MQ=255)                     ccAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTAGCCAGCGCCCAGGATCACTGGAATCGcc                                                                                                                      >  2:762654/1‑150 (MQ=255)                      cAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGGTAAATATTCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCa                                                                                                                     <  1:869437/150‑1 (MQ=255)                           tCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACaa                                                                                                                <  2:1020372/150‑1 (MQ=255)                           tCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACaa                                                                                                                >  1:1105356/1‑150 (MQ=255)                            cGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAAt                                                                                                               <  1:633212/150‑1 (MQ=255)                               gACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAg                                                                                                                                      >  2:404097/1‑124 (MQ=255)                               gACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGGCGCTGGCCAGCGTCCAg                                                                                                                                      <  1:404097/124‑1 (MQ=255)                                    tGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCTACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGNTGa                                                                                                       <  2:699705/150‑1 (MQ=255)                                       tCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCa                                                                                                    >  1:68122/1‑150 (MQ=255)                                                 cTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTc                                                                                          >  1:794375/1‑150 (MQ=255)                                                  tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCg                                                                                         >  2:1093655/1‑150 (MQ=255)                                                  tCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCg                                                                                         >  1:983413/1‑150 (MQ=255)                                                     aTCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGc                                                                                                                                                <  1:47173/92‑1 (MQ=255)                                                     aTCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGc                                                                                                                                                >  2:47173/1‑92 (MQ=255)                                                        cAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAg                                                                                   >  1:114859/1‑150 (MQ=255)                                                         aGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGa                                                                                  >  2:453048/1‑150 (MQ=255)                                                              tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                               >  2:490976/1‑100 (MQ=255)                                                              tcAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACt                                                                                                                               <  1:490976/100‑1 (MQ=255)                                                                    ccaaccaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGTGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATc                                                                       >  2:1011052/1‑150 (MQ=255)                                                                    ccaaccaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATc                                                                       >  1:49487/1‑150 (MQ=255)                                                                        ccaaAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGccc                                                                   <  2:49487/150‑1 (MQ=255)                                                                           aaGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGa                                                                <  1:1002473/150‑1 (MQ=255)                                                                                gCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCCGAAGGTGGTCATCGCCCAGATGCAc                                                           <  2:68122/150‑1 (MQ=255)                                                                                 cTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACg                                                          >  2:1024128/1‑150 (MQ=255)                                                                                        cAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCgg                                                   <  1:934424/150‑1 (MQ=255)                                                                                        cAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCgg                                                   <  1:934403/150‑1 (MQ=255)                                                                                              cACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGgacga                                             >  1:1033897/1‑150 (MQ=255)                                                                                                cccACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGacgacg                                           <  1:82394/150‑1 (MQ=255)                                                                                                 ccACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCACCTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACgg                                          >  2:757000/1‑150 (MQ=255)                                                                                                   acaGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGcc                                        >  2:940793/1‑150 (MQ=255)                                                                                                        acacGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCccgccg                                   <  2:206281/150‑1 (MQ=255)                                                                                                          acGCTGCGAATGGACGGAAACGCAAGACTGGACGCTAGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCACCGCCCAGATGCACGTACGCGGGGACGACGGCccgcgtac                                 <  1:762654/150‑5 (MQ=255)                                                                                                             cTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCgg                                                   >  2:994470/1‑129 (MQ=255)                                                                                                             cTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCgg                                                   <  1:994470/129‑1 (MQ=255)                                                                                                                  aaTGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCNACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGgac                                               <  1:260732/128‑1 (MQ=255)                                                                                                                  aaTGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCACAGGATCACTGGAATCGCCAGACAATGACGCTGAACAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGgac                                               >  2:260732/1‑128 (MQ=255)                                                                                                                   aTGAACGGAAACGCAAGTCGGGGCTCTGGCCAGCGCACAGGATCACTGGATTCGCCAGACAATGAAAGGGACCGAGGTCAACTCGGCGCAGACGGTGGGCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGtt                        <  1:346913/150‑1 (MQ=255)                                                                                                                     ggACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCt                      >  2:8390/1‑150 (MQ=255)                                                                                                                       aCGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGccc                                       >  2:103366/1‑131 (MQ=255)                                                                                                                       aCGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGccc                                       <  1:103366/131‑1 (MQ=255)                                                                                                                               gCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCTGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAggg            >  2:687153/1‑150 (MQ=255)                                                                                                                               gCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCccgccg                                   <  2:630517/127‑1 (MQ=255)                                                                                                                               gCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAggg            <  2:1105356/150‑1 (MQ=255)                                                                                                                               gCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAggg            >  2:1048886/1‑150 (MQ=255)                                                                                                                               gCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCGGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCccgccg                                   >  1:630517/1‑127 (MQ=255)                                                                                                                                cAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGc           <  1:940793/150‑1 (MQ=255)                                                                                                                                    aCTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtg                                                                             <  1:983908/80‑1 (MQ=255)                                                                                                                                    aCTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAggtg                                                                             >  2:983908/1‑80 (MQ=255)                                                                                                                                      tGGGCGCTGGCCAGCGTCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATc     >  2:744979/1‑150 (MQ=255)                                                                                                                                         aCGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCAcc  <  2:114859/150‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                      TGAAGCGATTGCCCGCTTTCCCAGCATCGTGACGGTGCTCAGCGATGGCTCGATCCAGTTCTCAGCCCCAACCAAAGGGGCTTCGAGCAAAAGCACCCACAGGACACGCTGCGAATGGACGGAAACGCAAGACTGGACGCTGGCCAGCGCCCAGGATCACTGGAATCGCCAGACAATGACGCTGACCAAGGTCAACTCGGCGCAGAAGGTGGTCATCGCCCAGATGCACGTACGCGGGGACGACGGCCCGCCGGTAAAGGTGTTCTGGAACAAGGGCAACATCACC  >  NC_002947/4300966‑4301251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑