Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,556,306 C→T 8.5% A79T (GCC→ACC)  zwfB ← glucose 6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,556,3060CT 8.5% 126.2 / 2.6 47A79T (GCC→ACC) zwfBglucose 6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (20/23);  new base T (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

ATAGAAAATGGCATTGCCGTGGCGGGTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCACGGCTGAGGTTGTACAGCGCGG  >  NC_002947/4556166‑4556452
                                                                                                                                            |                                                                                                                                                  
aTAGAAAATGGCATTGCCGTGGCGGGTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTGGGCGGATCGAGAAAGTCGCCCGTCTGGTAATCTAGGCGCTTGGCCAGACGGGCCCATAGCTGCTCGTCCAGGCACTTGGCGCTGCCCTc                                                                                                                                           >  2:379772/1‑150 (MQ=255)
      aaTGGCATTGCCGTGGCGGGTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCgccgcc                                                                                                                                     <  2:864943/150‑1 (MQ=255)
      aaTGGCATTGCCGTGGCGGGTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCgccgcc                                                                                                                                     >  1:133507/1‑150 (MQ=255)
             ttGCCGTGGCGGGTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTAACAGGATCGTGAATGTGACACGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCa                                                                                                                              >  2:942818/1‑150 (MQ=255)
                ccGTGGCGGGTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGc                                                                                                                           <  2:315669/150‑1 (MQ=255)
                     gCGGGTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAAGGACGTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGAc                                                                                                                      >  1:336453/1‑150 (MQ=255)
                        ggTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCAt                                                                                                                   >  1:1049207/1‑150 (MQ=255)
                        ggTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCAt                                                                                                                   <  2:844865/150‑1 (MQ=255)
                                 gATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTg                                                                                                          <  2:78181/150‑1 (MQ=255)
                                 gATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTg                                                                                                          <  1:632796/150‑1 (MQ=255)
                                 gATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTAGTATGCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCGCGCTCGACCATGAACGCGTg                                                                                                          <  1:562152/150‑1 (MQ=255)
                                         tGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGgcgcg                                                                                                  <  1:277747/150‑1 (MQ=255)
                                              aGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCgcggcg                                                                                             <  2:659284/150‑1 (MQ=255)
                                                             gCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCgg                                                                                   <  2:710129/145‑1 (MQ=255)
                                                             gCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCgg                                                                                   >  1:710129/1‑145 (MQ=255)
                                                             gCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTg                                                                              >  2:753304/1‑150 (MQ=255)
                                                                   tCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGGTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCCAGCGTGGGTATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCggg                                                                        <  1:476335/150‑1 (MQ=255)
                                                                     gagaAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATACCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCTCGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGtt                                                                      <  2:584703/150‑1 (MQ=255)
                                                                        aaaGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTCCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATg                                                                   <  2:857354/150‑1 (MQ=255)
                                                                            tCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCa                                                               >  2:363974/1‑150 (MQ=255)
                                                                                  gTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCTCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCt                                                                                      >  1:629489/1‑121 (MQ=255)
                                                                                  gTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCt                                                                                      <  2:629489/121‑1 (MQ=255)
                                                                                       gTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGTGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGAt                                                    >  1:134815/1‑150 (MQ=255)
                                                                                       gTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGTGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGAt                                                    >  1:134687/1‑150 (MQ=255)
                                                                                          aTCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATgcg                                                 >  1:926214/1‑150 (MQ=255)
                                                                                             gAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAg                                              <  1:1075095/150‑1 (MQ=255)
                                                                                                  gcTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTAc                                         >  2:213167/1‑150 (MQ=255)
                                                                                                   cTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACg                                        <  1:753304/150‑1 (MQ=255)
                                                                                                      ggCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTc                                     <  2:1064210/150‑1 (MQ=255)
                                                                                                      ggCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTc                                     <  1:213167/150‑1 (MQ=255)
                                                                                                        ccAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCa                                   >  1:415296/1‑150 (MQ=255)
                                                                                                         cAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAg                                  >  2:1084804/1‑150 (MQ=255)
                                                                                                          aGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGc                                 >  2:424082/1‑150 (MQ=255)
                                                                                                              gggCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGAGCAGGTTACGGTCCAGCAAAc                             >  1:126676/1‑150 (MQ=255)
                                                                                                                  ccATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTc                         >  1:123125/1‑150 (MQ=255)
                                                                                                                    aTAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGt                                                                                                           >  2:285432/1‑66 (MQ=255)
                                                                                                                    aTAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGt                                                                                                           <  1:285432/66‑1 (MQ=255)
                                                                                                                    aTAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCAc                       <  2:1049207/150‑1 (MQ=255)
                                                                                                                        cTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCACGGcc                   >  2:131128/1‑149 (MQ=255)
                                                                                                                          tctcGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCACGGCTGa                 >  2:72690/1‑150 (MQ=255)
                                                                                                                                       cTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCACGGCTGAGGTTGTACAgcgc    >  1:271677/1‑150 (MQ=255)
                                                                                                                                        ttGGTGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCACGGCTGAGGTTGTACAGcgcg   <  2:134687/150‑1 (MQ=255)
                                                                                                                                        ttGGTGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCACGGCTGAGGTTGTACAGcgcg   <  2:134815/150‑1 (MQ=255)
                                                                                                                                        ttGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCACGGCTGAGGTTGTACAGcgcg   <  1:1084804/150‑1 (MQ=255)
                                                                                                                                         tGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAGAGCCTCGGCGCTGGCCCGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCGAACCGTCACGGATGAGGTTGTACAGCGCgg  <  2:515976/150‑1 (MQ=255)
                                                                                                                                         tGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGAGCAGGTTACGGTCCAGCAAACCGTCACGGCTGAGGTTGTACAGCGCgg  <  2:126676/150‑1 (MQ=255)
                                                                                                                                         gggCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCACGGCTGAGGTTGTACAGCGCgg  <  1:338357/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
ATAGAAAATGGCATTGCCGTGGCGGGTCTTGTCGATGCGCCTGGCCAGGGCCTGGTAGGTGGCCGGATCGAGAAAGTCGCCCGTCTGGTAATCGAGGCGCTTGGCCAGGCGGGCCCATAGCTTCTCGTCCAGGCACTTGGCGCTGCCCTCGCCGCCTTTGTCACGCTCGACCATGAACGCGTGCAGGCGCGCGGCGAAATCCTCGGCGCTGGCCGGGTTATGGTCAACGCCGACGATGCGCAGGTTACGGTCCAGCAAACCGTCACGGCTGAGGTTGTACAGCGCGG  >  NC_002947/4556166‑4556452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑