| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,610,345 | C→G | 9.5% | A262A (GCC→GCG) | PP_4081 → | type IV/VI secretion system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,610,345 | 0 | C | G | 9.5% | 122.4 / 5.1 | 42 | A262A (GCC→GCG) | PP_4081 | type IV/VI secretion system protein |
| Reads supporting (aligned to +/- strand): ref base C (19/19); new base G (2/2); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
ATTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCGCT > NC_002947/4610199‑4610491 | aTTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATg > 2:846470/1‑150 (MQ=255) ccAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCATACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGt > 1:19699/1‑150 (MQ=255) cAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCCCCGCCATCGCCATGGCGGTg < 2:565405/150‑1 (MQ=255) atcCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTAc > 2:959443/1‑150 (MQ=255) atcCGCGAACGGCGCGCCCCCACCCCCGAAGAAGTCTGCGCCCCCTACACCAACGTCGCCCCCAGAAAACTCGCTGTGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTAc < 1:351447/150‑1 (MQ=18) aCTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATTCTCTTTTGGTGGAGCCCGTTGGTCATCTCCGCCATAGCCATGCGGCTGGCCCACGGGATTTACAGCTACCg > 1:192158/1‑150 (MQ=18) cTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGc < 1:846470/150‑1 (MQ=255) cgcgGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTGCGCACCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTg > 1:68430/1‑150 (MQ=255) gcgGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGc > 1:368708/1‑150 (MQ=255) ggCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTCGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCAc < 2:575792/150‑1 (MQ=255) ccACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGa < 1:259982/150‑1 (MQ=255) ccccGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATcac > 1:661059/1‑150 (MQ=255) cGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAAc > 1:769864/1‑150 (MQ=255) gaagTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAg < 2:985030/150‑1 (MQ=255) tGCGACCCATACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTg < 2:19699/150‑1 (MQ=255) cGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGa < 2:919233/150‑1 (MQ=255) ccTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGc > 1:773140/1‑150 (MQ=255) ccTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGc < 1:200904/150‑1 (MQ=255) ccccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca < 1:1022213/150‑1 (MQ=255) ccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCt < 2:145669/100‑1 (MQ=255) ccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCt > 1:145669/1‑100 (MQ=255) cAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgcagca > 1:148189/1‑150 (MQ=255) ttCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCCTCCCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCa > 2:427845/1‑150 (MQ=255) ccGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAAc > 2:470434/1‑108 (MQ=255) ccGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAAc < 1:470434/108‑1 (MQ=255) cGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGGCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAg > 1:979175/1‑150 (MQ=255) gtggtgGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGt < 2:368708/150‑1 (MQ=255) gtggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCa > 2:220908/1‑150 (MQ=255) tggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCaa < 1:533696/150‑1 (MQ=255) ggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAAc < 1:696655/150‑1 (MQ=255) gCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAAc < 1:229134/147‑1 (MQ=40) gCCCGTTGGTCATCTCCGCCATAGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAAc > 2:229134/1‑147 (MQ=255) ggTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACTGTGTCACAGCACCACTGAGTTCAACGAATTCGCGa < 2:595503/150‑1 (MQ=38) tCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAgg > 1:626589/1‑150 (MQ=37) tctcCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCAt < 1:807581/91‑1 (MQ=37) tctcCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCAt > 2:807581/1‑91 (MQ=37) tctcCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGttt > 1:459402/1‑150 (MQ=34) ccaccGCCATGGCGATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGttt < 1:392189/147‑1 (MQ=12) caccGCCATGGCGATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAAt < 2:433014/99‑1 (MQ=255) caccGCCATGGCGATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAAt > 1:433014/3‑101 (MQ=255) caccGCCATGGCGATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGtttt > 2:428798/3‑150 (MQ=12) tcCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGCAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGttttt < 2:769864/150‑1 (MQ=17) cgccatGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCGCt < 2:661059/150‑1 (MQ=32) | ATTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCGCT > NC_002947/4610199‑4610491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |