Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 359,603 A→G 7.1% Q356R (CAG→CGG)  gbdR → AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947359,6030AG 7.1% 159.7 / 2.8 56Q356R (CAG→CGG) gbdRAraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (28/24);  new base G (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

GCACTTCTCCAAGTGCTATCGCGAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTG  >  NC_002947/359454‑359752
                                                                                                                                                     |                                                                                                                                                     
gCACTTCTCCAAGTGCTATCGCGAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCa                                                                                                                                                       <  1:662641/150‑1 (MQ=255)
       tcCAAGTGCTATCGCGAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGgcgcgc                                                                                                                                                >  1:633482/1‑150 (MQ=255)
         cAAGTGCTATCGCGAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCaa                                                                                                                                              >  1:987011/1‑150 (MQ=255)
          taGTGCTATCGCGAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACGACGGTCCGATGGCGGCCCTGAGCCAGGCGCGCAAt                                                                                                                                             >  2:932516/2‑150 (MQ=255)
               cTATCGCTAGTACTTCTGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCTAGGCGCGCAATGAGTc                                                                                                                                        <  2:569949/150‑1 (MQ=255)
               cTATCGCGAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTc                                                                                                                                        <  1:501268/150‑1 (MQ=255)
                tATCGCGAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCGGGCGCGCAATGAGTCg                                                                                                                                       >  2:421891/1‑150 (MQ=255)
                tATCGCGAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCGGGCGCGCAATGAGTCg                                                                                                                                       >  2:421719/1‑150 (MQ=255)
                     cgAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGgcgc                                                                                                                                                  <  1:910938/134‑1 (MQ=255)
                     cgAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGgcgc                                                                                                                                                  >  2:910938/1‑134 (MQ=255)
                            ttCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCCACTCCCCGCAGCAGGTGGCCATGATGCCGATTAAGCTTCCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAgt                                                                                                                           <  1:518953/150‑1 (MQ=255)
                            ttCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAgt                                                                                                                           >  1:930272/1‑150 (MQ=255)
                            ttCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAgt                                                                                                                           >  2:9334/1‑150 (MQ=255)
                            ttCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAgt                                                                                                                           >  2:930497/1‑150 (MQ=255)
                              cGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAgtgt                                                                                                                         >  2:485420/1‑150 (MQ=255)
                                     ccgccgCGTGACGAGCGGGTAGGCTCCAACACCGCGCAACAGGTGGCCATGATGCCCTCTCCGAAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGctc                                                                                                                  <  1:12350/150‑1 (MQ=255)
                                                 gAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATACTCGTGCTTGCATTCCGCATTACCCTGTCGGCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGcc                                                                                                      >  1:861707/1‑150 (MQ=255)
                                                      ggTAGGCTCCAACACCGCGCAGCAGGTGGCCATGGTGAAGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGAGGGCTGGCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCct                                                                                                 >  2:680491/1‑150 (MQ=255)
                                                        tAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGt                                                                                                                                         <  2:268162/108‑1 (MQ=255)
                                                        tAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGt                                                                                                                                         >  1:268162/1‑108 (MQ=255)
                                                         aGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCCATTTCGCGGGGCAAGGCGCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCtt                                                                                              >  1:22167/1‑150 (MQ=255)
                                                           gCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTcg                                                                                            >  2:449947/1‑150 (MQ=255)
                                                               cAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCgga                                                                                        <  1:485420/150‑2 (MQ=255)
                                                                aaCACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCGCAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGc                                                                                       >  2:259660/1‑150 (MQ=255)
                                                                 acacCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGa                                                                                                               <  1:935585/125‑1 (MQ=255)
                                                                 acacCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGa                                                                                                               >  2:935585/1‑125 (MQ=255)
                                                                 acacCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGATCTGACACCGCGCCGGCCTCTTCGCgg                                                                                         >  1:388838/1‑147 (MQ=255)
                                                                 acacCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGATCTGACACCGCGCCGGCCTCTTCGCgg                                                                                         <  2:388838/147‑1 (MQ=255)
                                                                  cacCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCAc                                                                                     >  2:328619/1‑150 (MQ=255)
                                                                   acCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACg                                                                                    >  2:903971/1‑150 (MQ=255)
                                                                      gcgcAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGccc                                                                                 >  2:141293/1‑150 (MQ=255)
                                                                        gcagcaGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGc                                                                               >  2:799360/1‑150 (MQ=255)
                                                                          agcagGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTc                                                                             <  1:746073/150‑1 (MQ=255)
                                                                               gTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGctc                                                                                                                  >  2:186751/1‑108 (MQ=255)
                                                                               gTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGctc                                                                                                                  <  1:186751/108‑1 (MQ=255)
                                                                               gTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCcaca                                                                        >  1:460833/1‑150 (MQ=255)
                                                                                     atgatgCCGATTCCGCAGGCGATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCAc                                                                  >  1:562946/1‑150 (MQ=255)
                                                                                     atgatgCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCcaca                                                                        <  2:107873/144‑1 (MQ=255)
                                                                                     atgatgCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCcaca                                                                        >  1:107873/1‑144 (MQ=255)
                                                                                     atgatgCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCAc                                                                  <  2:1014936/150‑1 (MQ=255)
                                                                                            cGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGa                                                           <  1:1020332/150‑1 (MQ=255)
                                                                                             gATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAg                                                          >  2:807540/1‑150 (MQ=255)
                                                                                                  cGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGa                                                     <  2:1074232/150‑1 (MQ=255)
                                                                                                                    ggccgcACGGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTgg                                   <  2:175877/149‑1 (MQ=255)
                                                                                                                    cgccgcACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTgg                                   <  1:903971/150‑1 (MQ=255)
                                                                                                                        gcACAGCGGGCCGATGGCGGCCCTGAGCCGGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGgggc                               <  1:421719/150‑1 (MQ=255)
                                                                                                                        gcACAGCGGGCCGATGGCGGCCCTGAGCCGGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGgggc                               <  1:421891/150‑1 (MQ=255)
                                                                                                                          acaGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGgggcgg                             <  1:449947/150‑1 (MQ=255)
                                                                                                                            aGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGgggcgggc                           <  2:22167/150‑1 (MQ=255)
                                                                                                                                 gCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGtt                      >  1:838927/1‑150 (MQ=255)
                                                                                                                                   cGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGCGTTGTTcc                    >  2:283077/1‑150 (MQ=255)
                                                                                                                                        gcggcCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGaa               <  2:861707/150‑1 (MQ=255)
                                                                                                                                        gcggcCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGaa               <  2:987011/150‑1 (MQ=255)
                                                                                                                                         cggcCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAg              <  2:1012693/150‑1 (MQ=255)
                                                                                                                                             ccTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGc          <  1:793671/150‑1 (MQ=255)
                                                                                                                                             ccTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGc          <  2:633482/150‑1 (MQ=255)
                                                                                                                                              cTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGcc         >  1:604592/1‑150 (MQ=255)
                                                                                                                                                     aGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTg  <  2:862044/150‑1 (MQ=255)
                                                                                                                                                     aGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTg  <  1:259660/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCACTTCTCCAAGTGCTATCGCGAGTACTTCGGTATTCCGCCGCGTGACGAGCGGGTAGGCTCCAACACCGCGCAGCAGGTGGCCATGATGCCGATTCCGCAGGCCATGACCCTGTCGCCGCACAGCGGGCCGATGGCGGCCCTGAGCCAGGCGCGCAATGAGTCGACATTTGCCAGTGTAAGGCTCTGACACCGCGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTCACCCGCTGAGCTTGAGCCAGCGGTGATCTGTGGGGGCGGGCTTGTTCCGCGAAGGGGCCGGTCCTG  >  NC_002947/359454‑359752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑