| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,731,020 | T→C | 10.5% | D157G (GAC→GGC) | lpdG ← | dihydrolipoyl dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,731,020 | 0 | T | C | 10.5% | 99.3 / 3.1 | 38 | D157G (GAC→GGC) | lpdG | dihydrolipoyl dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (17/17); new base C (2/2); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01 | |||||||||||
TTCAGCACCCAGGCGAGCCCATACCGAACCCAGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGA > NC_002947/4730872‑4731161 | ttCAGCACCCAGGCGAGCCCATACCGAACCCAGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTc > 2:854697/1‑150 (MQ=255) cAGCACCCAGGCGAGCCCATACCGAACCCAGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGa > 1:637182/1‑150 (MQ=255) ccAGGCGAGCCCATACCGAACCCAGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGgc < 2:372241/150‑1 (MQ=255) gAGCCCATACCGAACCCAGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGc > 2:111026/1‑150 (MQ=255) tACCGAACCCCGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGc < 2:282952/150‑1 (MQ=255) ccGAACCCAGCTCCAGGCCGATCACGCCTGGACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCa > 2:655602/1‑150 (MQ=255) cGAACCCAGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAg < 2:637182/150‑1 (MQ=255) ccAGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGCAGCCAGGATCa > 1:706229/1‑150 (MQ=255) ccAGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCa > 2:419476/1‑150 (MQ=255) ccAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGCGTTTATTTTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTtct < 1:618996/150‑1 (MQ=255) cACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGAt < 1:111026/150‑1 (MQ=255) aCGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATg > 2:746225/1‑150 (MQ=255) cTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATc > 2:821377/1‑128 (MQ=255) cTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATc < 1:821377/128‑1 (MQ=255) cTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACtt > 2:590289/1‑150 (MQ=255) cTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACtt > 1:947251/1‑150 (MQ=255) ccGTTAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGCTCGGGCGCGAGCCGGAGGCACGGATGCCGTACCCGgcgtcggtggcgtcggtg > 1:417524/1‑150 (MQ=255) cGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCggtgg > 1:593821/1‑150 (MQ=255) gATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCggtggt < 2:599808/150‑1 (MQ=255) cGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGt < 2:593821/150‑1 (MQ=255) gcTTCGGTACGGCCTGGAATTCCAGGGCGCCCGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTgg < 2:581941/150‑1 (MQ=255) cTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGt < 1:274503/150‑1 (MQ=255) ttCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGCCGATCgg < 1:805130/83‑1 (MQ=255) ttCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGCCGATCgg < 1:805037/83‑1 (MQ=255) ttCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGCCGATCgg > 2:805037/1‑83 (MQ=255) ttCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGCCGATCgg > 2:805130/1‑83 (MQ=255) cTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGTTGGTGCCGTCAGCCTTCGGGACTTCGACttt > 1:785139/1‑150 (MQ=255) tGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCt < 1:1019481/105‑1 (MQ=255) tGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCt > 2:1019481/1‑105 (MQ=255) ccGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGc < 2:462662/64‑1 (MQ=255) ccGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGc > 1:462662/1‑64 (MQ=255) gacgaTGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGcc < 1:419476/150‑1 (MQ=255) aTGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTgg > 1:1004370/1‑150 (MQ=255) gACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGAt < 2:65729/150‑1 (MQ=255) ggggCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTg < 2:1004370/150‑1 (MQ=255) ccGGCGGAATGTCGATCGGGCGTGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTTGCCt > 2:138756/1‑150 (MQ=255) cggcggAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCtt < 1:655602/150‑1 (MQ=255) gcggAATGTCGAGCGGGCGCGACCCGGAAGCCAGGAGCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGa < 2:333075/150‑1 (MQ=255) tCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGt > 1:992702/1‑109 (MQ=255) tCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGt < 2:992702/109‑1 (MQ=255) | TTCAGCACCCAGGCGAGCCCATACCGAACCCAGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGA > NC_002947/4730872‑4731161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |