| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,758,181 | G→C | 13.6% | R470P (CGC→CCC) | ompQ → | outer membrane pyoverdine efflux protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,758,181 | 0 | G | C | 13.6% | 121.6 / 11.5 | 45 | R470P (CGC→CCC) | ompQ | outer membrane pyoverdine efflux protein |
| Reads supporting (aligned to +/- strand): ref base G (20/18); new base C (4/2); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
AGACCTTGCTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGT > NC_002947/4758037‑4758325 | agaCCTTGCTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAgg < 1:1000703/150‑1 (MQ=255) gCTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAg < 1:39432/150‑1 (MQ=255) gTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCt > 1:454997/1‑150 (MQ=255) acCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCTTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCAc < 1:341490/150‑1 (MQ=255) acCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCAc > 2:477769/1‑150 (MQ=255) cccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACg > 1:606002/1‑150 (MQ=255) ccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCCACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGa < 2:606002/150‑1 (MQ=255) ccTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCt < 2:1038725/150‑1 (MQ=255) tGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGa > 1:1003678/1‑150 (MQ=255) ccAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCtt > 1:967777/1‑150 (MQ=255) ccAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCtt > 1:701598/1‑150 (MQ=255) cccAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt < 1:850457/150‑1 (MQ=255) aGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTcggg > 2:853964/1‑107 (MQ=255) aGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTCggg < 1:853964/110‑1 (MQ=255) cccGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACGAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAgg < 2:989855/150‑1 (MQ=255) ccGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAggg > 1:1000589/1‑150 (MQ=255) tGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGa < 2:4169/109‑1 (MQ=255) tGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGa > 1:4169/1‑109 (MQ=255) cAGCAAAGGCAACTTTAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGAGCTTCAGGTGGCGAGCATACCAGGGgcggcgc < 2:613118/150‑1 (MQ=255) aaGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCAc < 2:1000589/150‑1 (MQ=255) ccAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGGCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCCGGTGGCGAGCATACCAGGGGCGGCCCGGACCttt > 2:174879/1‑150 (MQ=255) aaCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGc < 1:477769/150‑1 (MQ=255) cAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGccc < 1:780852/150‑1 (MQ=255) cGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTc > 2:960623/1‑150 (MQ=255) tGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCaa > 2:3939/1‑150 (MQ=255) ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTNaaa < 2:701598/150‑1 (MQ=255) ggCCCTTTACAAAGCCGTGGGCGGTGGCTGGCAAGCGGATAGCCCGACTCTCTAGGGCGTCAGGCTAGCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCaaa < 2:170849/150‑1 (MQ=255) gCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAg > 2:256029/1‑150 (MQ=255) tttACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGct > 2:72370/1‑150 (MQ=255) tACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcc > 2:758017/1‑150 (MQ=255) ttGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAg < 1:233214/83‑1 (MQ=255) ttGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAg > 2:233214/1‑83 (MQ=255) tggctggcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAg < 2:1003678/150‑1 (MQ=255) gctggcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGgc < 1:3939/150‑1 (MQ=255) cAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAg < 1:758017/150‑1 (MQ=255) aaGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAgg > 2:945296/1‑150 (MQ=255) cGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCa > 2:589644/1‑150 (MQ=255) cctgACGCCCTAGCGCGTCGGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTTGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCAt > 1:955120/4‑150 (MQ=255) cctgACGCCCTAGCGCGTCGGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcctc < 2:901078/114‑1 (MQ=255) cctgACGCCCTAGCGCGTCGGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcctc > 1:901078/4‑117 (MQ=255) cctgACGCCCTAGCGCGTCGGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCAt > 2:1008428/4‑150 (MQ=255) cccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCAt < 1:989447/150‑1 (MQ=255) cccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGAAGCGGCACTTTGCCTACCAGCCAGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCAt > 1:251455/1‑150 (MQ=255) ccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATg > 1:315539/1‑150 (MQ=255) gACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACCTCTCCTACCATCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGTCGCCAGACCAGCGTCATGGt > 2:34819/1‑150 (MQ=255) | AGACCTTGCTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGT > NC_002947/4758037‑4758325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |