Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,848,642 G→T 7.1% intergenic (+2/‑104) PP_4263 → / → hemN cytochrome biogenesis protein/oxygen‑independent coproporphyrinogen III dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,848,6420GT 7.1% 176.0 / 2.5 56intergenic (+2/‑104)PP_4263/hemNcytochrome biogenesis protein/oxygen‑independent coproporphyrinogen III dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (24/28);  new base T (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GTGTTGCTGGCAACCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGATCC  >  NC_002947/4848494‑4848782
                                                                                                                                                    |                                                                                                                                            
gtgtTGCTGGCAACCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGNCgg                                                                                                                                             <  2:698123/150‑1 (MQ=255)
 tgtTGCTGGCAACTGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACggg                                                                                                                                            <  1:140259/150‑1 (MQ=255)
 tgtTGCTGGCAACCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACggg                                                                                                                                            <  1:258471/150‑1 (MQ=255)
   ttGCTGGCAACCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACggggg                                                                                                                                          <  2:3616/150‑1 (MQ=255)
        ggCAGCCAGGCTTTCTGCCGAACGGATGAACGCCCGGTTGCGGCGACGCGGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCa                                                                                                                                     >  2:884111/1‑150 (MQ=255)
           aaCCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGctttg                                                                                                                                  >  2:852697/1‑146 (MQ=255)
                 gctggctgCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGccc                                                                                                                            >  2:855018/1‑150 (MQ=255)
                         ccGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGccc                                                                                                                    >  2:1013499/1‑150 (MQ=255)
                           gAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCtt                                                                                                                  >  1:1129530/1‑150 (MQ=255)
                           gAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCtt                                                                                                                  <  2:918284/150‑1 (MQ=255)
                             aCGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGa                                                                                                                <  1:587357/150‑1 (MQ=255)
                               ggATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCGGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATa                                                                                                              <  2:919369/150‑1 (MQ=255)
                               gctggAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCCCTGCCTGATAGGCCATTGGCGAGGACCGGAAAGCCGCCCCAATGCCCTTGGTa                                                                                                              >  1:879548/5‑150 (MQ=255)
                                 aTGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGctttg                                                                                                                                  >  1:594968/1‑124 (MQ=255)
                                 aTGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGctttg                                                                                                                                  <  2:594968/128‑5 (MQ=255)
                                   gAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTg                                                                                                                 >  1:362072/1‑143 (MQ=255)
                                   gAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTg                                                                                                                 <  2:362072/143‑1 (MQ=255)
                                   gAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCCTGCCCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACaaa                                                                                                          <  1:912089/150‑1 (MQ=255)
                                         ccTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAaca                                                                                                    >  1:276123/1‑150 (MQ=255)
                                           tGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAAcaca                                                                                                  <  1:1013499/150‑1 (MQ=255)
                                           tGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAAcaca                                                                                                  <  2:112166/150‑1 (MQ=255)
                                             ttGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGt                                                                                                >  1:715124/1‑150 (MQ=255)
                                                 ggcgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTcc                                                                                            <  1:473091/150‑1 (MQ=255)
                                                 ggcgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTcc                                                                                            <  1:599640/150‑1 (MQ=255)
                                                  gcgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCt                                                                                           >  2:731294/1‑150 (MQ=255)
                                                  gcgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCt                                                                                           >  2:603328/1‑150 (MQ=255)
                                                   cgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTa                                                                                          <  1:1025085/150‑1 (MQ=255)
                                                     aCGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACa                                                                                        <  1:212000/150‑1 (MQ=255)
                                                        cAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGTCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGAc                                                                                     >  1:412165/1‑150 (MQ=255)
                                                        cAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGAc                                                                                     <  2:654031/150‑1 (MQ=255)
                                                                   tGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACt                                                                          >  2:775011/1‑150 (MQ=255)
                                                                        ggCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCACACGATAACACGGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGt                                                                     >  1:922653/1‑150 (MQ=255)
                                                                                           tATCCGGTAGCGGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCgg                                                  <  2:284193/150‑1 (MQ=255)
                                                                                            aTTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCggg                                                 <  1:609316/150‑1 (MQ=255)
                                                                                            aTTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCggg                                                 <  1:855018/150‑1 (MQ=255)
                                                                                              tCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGa                                               <  2:909353/150‑1 (MQ=255)
                                                                                                      tGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTAccc                                         <  2:123458/148‑1 (MQ=255)
                                                                                                      tGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTAccc                                         >  1:123458/1‑148 (MQ=255)
                                                                                                        gACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCAt                                     <  1:603328/150‑1 (MQ=255)
                                                                                                           ccTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCt                                  >  2:95035/1‑150 (MQ=255)
                                                                                                             tGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCggg                                                 <  1:614416/133‑1 (MQ=255)
                                                                                                             tGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCggg                                                 >  2:614416/1‑133 (MQ=255)
                                                                                                               ccGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTcgac                              <  1:95035/150‑1 (MQ=255)
                                                                                                                 gggCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGAc                                                                                     >  1:899293/1‑93 (MQ=255)
                                                                                                                 gggCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGAc                                                                                     <  2:899293/93‑1 (MQ=255)
                                                                                                                     ccACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTc                        >  1:611175/1‑150 (MQ=255)
                                                                                                                      cacaCCAGCACTGGCTGATGGGCCATTGACTGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGAc                                                                           <  2:211054/98‑1 (MQ=255)
                                                                                                                      cacaCCAGCACTGGCTGATGGGCCATTGACTGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGAc                                                                           >  1:211054/1‑98 (MQ=255)
                                                                                                                      cacaCCAGCACTGGCTGATGGGCCATTGACTGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGAc                                                                           <  2:210994/98‑1 (MQ=255)
                                                                                                                      cacaCCAGCACTGGCTGATGGGCCATTGACTGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGAc                                                                           >  1:210994/1‑98 (MQ=255)
                                                                                                                         acCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGtt                    <  2:1129530/150‑1 (MQ=255)
                                                                                                                         acCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCCtt                    >  2:196261/1‑150 (MQ=255)
                                                                                                                           cAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTgg                  <  1:884111/150‑1 (MQ=255)
                                                                                                                             gCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGa                <  1:731294/150‑1 (MQ=255)
                                                                                                                             gCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGa                >  2:198440/1‑150 (MQ=255)
                                                                                                                               aCTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCActg                                                               <  2:579819/101‑1 (MQ=255)
                                                                                                                               aCTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCActg                                                               >  1:579819/1‑101 (MQ=255)
                                                                                                                                   gCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACTGCCATAGACTCCGGTGCACTAATGCCCTCTGGATGTGCATCCCTCATGCTCGACGACCTCCGTTGGGATACCGa          >  1:247759/1‑150 (MQ=255)
                                                                                                                                          ggCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGATc   <  1:775011/150‑1 (MQ=255)
                                                                                                                                           gCCATTGACGGGGGCTGCAAAGCAGCCTCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACTACCTCCGTTGGGATACCGACatcatca  <  2:276123/150‑2 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GTGTTGCTGGCAACCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCCACACCAGCACTGGCTGATGGGCCATTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCGACCTGATCC  >  NC_002947/4848494‑4848782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑