Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,850,942 T→A 10.0% F210I (TTC→ATC) ‡ fnrA → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,850,9420TA10.0% 115.3 / 5.0 40F210I (TTC→ATC) ‡fnrADNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (16/20);  new base A (2/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

ACGAGCGCATTGCCACCTTCCTGGTCAACCTGTCGGCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCGGGTATACTTGGGCATTCGTTTTCC  >  NC_002947/4850799‑4851088
                                                                                                                                               |                                                                                                                                                  
acgaGCGCATTGCCACCTTCCTGGTCAACCTGTCTGCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCAccc                                                                                                                                              <  1:965052/150‑1 (MQ=255)
       taTTACCACCTCCCCGGCCAACCTGTCGTCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGGGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAACCCGTGTCGCGGGTGTTCACCCGCTTCca                                                                                                                                       <  2:905315/149‑1 (MQ=255)
       cATTGCCACCTTCCTGGTCAACCTGTCGGCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCca                                                                                                                                       <  2:405082/150‑1 (MQ=255)
              aCCTTCCTGGTCAACCTGTCGGCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAAc                                                                                                                                >  2:920037/1‑150 (MQ=255)
                 ttCCTGGTCAACCTGTCGGCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGc                                                                                                                             <  1:236871/150‑1 (MQ=255)
                 ttCCTGGTCAACCTGTCGGCGCGCTTCCGCGCCCGAGGCTGATGGACCAATCATTGCCGCCTGAGCATGCCGCCTAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGc                                                                                                                             <  1:248099/150‑1 (MQ=255)
                          aaCCTGTCGGCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTgcgc                                                                                                                    >  1:733576/1‑150 (MQ=255)
                              tGTCGGCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCGGCTGCGCGCCg                                                                                                                >  2:79988/1‑150 (MQ=255)
                                 cGGCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAgg                                                                                                             <  1:990015/150‑1 (MQ=255)
                                    cgcgcTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCGGCGCGCCGAGGGCa                                                                                                          >  2:1106656/1‑150 (MQ=255)
                                         ttCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCGTGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGaa                                                                                                     >  2:1062329/1‑150 (MQ=255)
                                              cgcCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGca                                                                                                <  2:361499/150‑1 (MQ=255)
                                                 ccGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACAt                                                                                             >  1:659675/1‑150 (MQ=255)
                                                     ggCCATTCGTCCAACCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAACCCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCCCGCCGAGGGCAAGGAAGTGCACATCCTc                                                                                         <  2:283646/150‑1 (MQ=255)
                                                        tATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGAc                                                                                      <  1:641812/150‑1 (MQ=255)
                                                          ttCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTAGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGca                                                                                                <  2:886810/138‑1 (MQ=255)
                                                          ttCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTAGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGca                                                                                                >  1:886810/1‑138 (MQ=255)
                                                                   tCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCt                                                                           <  1:1106656/150‑1 (MQ=255)
                                                                      gTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCt                                                                           <  1:865304/147‑1 (MQ=255)
                                                                      gTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCt                                                                           >  2:865304/1‑147 (MQ=255)
                                                                       ttCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTgc                                                                       >  1:771657/1‑150 (MQ=255)
                                                                            ccTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCt                                                                  >  2:845328/1‑150 (MQ=255)
                                                                              tGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTgg                                                                >  2:7639/1‑150 (MQ=255)
                                                                                    tGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAggtg                                                          <  1:612802/150‑1 (MQ=255)
                                                                                    tGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAggat                                                          <  1:1062329/150‑3 (MQ=255)
                                                                                       cgcgCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGgtggtg                                                       <  1:441522/150‑1 (MQ=255)
                                                                                                          ttACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTg                                    >  2:655317/1‑150 (MQ=255)
                                                                                                              cTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCt                                >  2:489549/1‑150 (MQ=255)
                                                                                                                     tGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCgg                         >  1:498208/1‑150 (MQ=255)
                                                                                                                     gggCGGTAGAATCCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACAGCCTCGACCCGAGCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCgg                         <  2:32946/149‑1 (MQ=255)
                                                                                                                        cGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCGGGta                      <  1:920037/150‑1 (MQ=255)
                                                                                                                        cGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCGGGta                      <  1:489549/150‑1 (MQ=255)
                                                                                                                             gAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGATGGTCTTTAGCGGGTATACtt                 >  2:634512/1‑150 (MQ=255)
                                                                                                                               aaCCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCGGGTATACTTgg               <  2:659675/150‑1 (MQ=255)
                                                                                                                                   tggaagcgGGTGAACACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCGGGTATACTTGGGCAt           >  2:787683/6‑150 (MQ=255)
                                                                                                                                   tggaagcgGGTGAACACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCGGGTATACTTGGGCAt           <  1:787683/145‑1 (MQ=255)
                                                                                                                                     gtCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCGGGTATACTTGGGCATTc         <  1:7639/150‑1 (MQ=255)
                                                                                                                                      aagcgGGTGAACACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTc                                 <  2:280116/123‑1 (MQ=255)
                                                                                                                                      aagcgGGTGAACACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGATGGTc                                 >  1:280116/3‑125 (MQ=255)
                                                                                                                                          gggTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGAATAATATTGGTCTTTAGCGGGTATACTTGGGCATTCGtttt    >  1:336182/1‑150 (MQ=255)
                                                                                                                                            ttgtTCACCCGTTGCTAGCAGAACGGCCTGGTGCTCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGGGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCGCGTATACTTGTGCATTCGTTTTct  <  2:383592/149‑2 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
ACGAGCGCATTGCCACCTTCCTGGTCAACCTGTCGGCGCGCTTCCGCGCCCGCGGCTATTCGGCCAATCAGTTCCGCCTGAGCATGTCGCGCAACGAAATCGGCAATTACCTTGGCCTGGCGGTAGAAACCGTGTCGCGGGTGTTCACCCGCTTCCAGCAGAACGGCCTGCTGCGCGCCGAGGGCAAGGAAGTGCACATCCTCGACCCGATCCAGCTGTGCGCGCTGGCAGGTGGTGCAATCGAGGCCTGACGCTGGTCTTTAGCGGGTATACTTGGGCATTCGTTTTCC  >  NC_002947/4850799‑4851088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑