| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,891,481 | G→A | 6.6% | V200M (GTG→ATG) | ttuD → | hydroxypyruvate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,891,481 | 0 | G | A | 6.6% | 169.5 / 2.7 | 61 | V200M (GTG→ATG) | ttuD | hydroxypyruvate reductase |
| Reads supporting (aligned to +/- strand): ref base G (28/29); new base A (2/2); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01 | |||||||||||
CATCGGCGAGATGAACTGCGTGCGCAAGCACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGCCGATGACCCGGCCCTGGCCCGCAGCCACTT > NC_002947/4891333‑4891626 | caTCGGCGAGATGAACTGCGTGCGCAAGCACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGt < 2:135050/150‑1 (MQ=255)caTCGGCGAGATGAACTGCGTGCGCAAGCACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGt < 2:743399/150‑1 (MQ=255) aTCGGCGAGATGAACTGCGTGCGCAAGCACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTg < 2:28100/150‑1 (MQ=255) cGAGATGAACTGCGTGCGCAAGCACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGa > 1:33121/1‑150 (MQ=255) aTGAACTGCGTGCGCAAGCACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCg > 2:709539/1‑150 (MQ=255) gAACTGCGTGCGCAAGCACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCGACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAg < 2:314172/150‑1 (MQ=255) gAACTGCGTGCGCAAGCACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAg > 2:82352/1‑150 (MQ=255) tgcgCAAGCACCTCTCGGCGATCAAGATCGAGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCgg > 1:759549/1‑150 (MQ=255) aaGCACCTCTCGGCGATCAAGGGCAGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGTCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGAc < 2:319160/150‑1 (MQ=255) cACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGcc < 1:787322/150‑1 (MQ=255) ctctCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTg > 1:485464/1‑150 (MQ=255) tctcGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTgg < 1:82352/150‑1 (MQ=255) ctcGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCGGGCCGGCGACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCCCCGGCCCCACCGTGGCCGACCCGAGCTCCTCGTCCGAGGCCCTGGc < 2:444210/150‑1 (MQ=255) ggCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCAt > 1:394108/1‑150 (MQ=255) gCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCttgatcgc > 1:232032/1‑143 (MQ=255) gCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATc < 1:500638/150‑1 (MQ=255) gCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATc < 2:1012877/150‑1 (MQ=255) gATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCt > 1:276139/1‑150 (MQ=255) cAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCGTCCTCaa > 1:121833/1‑150 (MQ=255) aGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCGCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAAc < 1:350501/150‑1 (MQ=255) cgggcgCCTGGCCAGGCCCGGCTCGCCGGCCCCGGTCTACACCTATGCCCTTTCCGAGGTACCGCGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGCCCGACCCGCGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTa < 2:234992/150‑1 (MQ=255) cgggcgCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTa < 1:389006/150‑1 (MQ=255) gggcgCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTac > 1:221405/1‑150 (MQ=255) ctgaacAAGGCCTGCTAGGCGGCCACTGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATc > 2:1123004/6‑150 (MQ=255) cTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATc > 1:1100390/1‑150 (MQ=255) gCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGaa < 2:485464/150‑1 (MQ=255) ccAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAg > 1:480603/1‑150 (MQ=255) cAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAgc < 2:132943/150‑1 (MQ=255) aaGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAgcg > 2:950840/1‑150 (MQ=255) aaGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAgcg > 1:337704/1‑150 (MQ=255) aaGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAgcg < 1:267229/150‑1 (MQ=255) ccTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGAAGGACCGGGCGACCTCGCCACGGTAATCCCCTCCGGCACCACCGTGGCTGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACAACGAAGCGCCCa > 1:630099/1‑150 (MQ=255) tgctgGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCaaa > 1:638011/1‑150 (MQ=255) cggccACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGCCCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCa > 1:730432/1‑150 (MQ=255) cggccACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCa < 1:1123004/150‑1 (MQ=255) cggccACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCa < 2:221405/150‑1 (MQ=255) gccACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATc < 2:638011/150‑1 (MQ=255) cACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGa < 2:480603/150‑1 (MQ=255) aCGGTCTACACATATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGAc > 2:910625/1‑150 (MQ=255) tATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGaacaac > 1:369310/1‑150 (MQ=255) cATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGc < 2:1100390/150‑1 (MQ=255) cGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGa > 2:349968/1‑150 (MQ=255) tgccccGCGCGCCCTCGCCACGCTAACCGCCTCCGTCCCCACCGTGGGCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAACCGCTACAACATCGAAGCGCCCAAAGCGGTCATAGACTGGCTGAACAACCCGGCCTCGGAAAc < 2:121833/146‑1 (MQ=255) ccGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAAccc < 2:839633/134‑1 (MQ=255) ccGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAAccc > 1:839633/1‑134 (MQ=255) ggCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAg > 1:386699/1‑150 (MQ=255) gACCTCGCCACGGTAATCGCCTCCGGAGCCGTCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGcc > 1:578843/1‑150 (MQ=255) ccTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGCGAACTCATGGCGTCTCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGCCGa < 2:578843/150‑1 (MQ=255) cGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGCCGATGa < 2:276139/150‑1 (MQ=255) aCGGTAATCGCCTCCGGCCCCACCATGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAg > 1:476630/1‑138 (MQ=255) aCGGTAATCGCCTCCGGCCCCACCATGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAg > 1:476780/1‑138 (MQ=255) aCGGTAATCGCCTCCGGCCCCACCATGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAg < 2:476630/138‑1 (MQ=255) aCGGTAATCGCCTCCGGCCCCACCATGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAg < 2:476780/138‑1 (MQ=255) aaTCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAGTCCTTGCTCGGAAACCATCAAGGCCTGGGACGCGGCCCt > 1:57626/1‑150 (MQ=255) ccTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGCCGATGACCCGGCCCTGGccc > 1:84465/1‑150 (MQ=255) gCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGCCGATGACCCGGCCCTGGCCCGCAGcc < 1:697423/150‑1 (MQ=255) cccACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAgg < 2:1085320/121‑1 (MQ=255) cccACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAgg > 1:1085320/1‑121 (MQ=255) cACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGCCGATGACCCGGCCCTGGCCCGCAGCCACtt < 2:655791/150‑1 (MQ=255) ccGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCACCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGCCGATGa < 2:140288/124‑1 (MQ=255) ccGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCACCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGCCGATGa > 1:140288/1‑124 (MQ=255) | CATCGGCGAGATGAACTGCGTGCGCAAGCACCTCTCGGCGATCAAGGGCGGGCGCCTGGCCAAGGCCTGCTGGCCGGCCACGGTCTACACCTATGCCATTTCCGATGTACCGGGCGACCTCGCCACGGTAATCGCCTCCGGCCCCACCGTGGCCGACCCGAGCACCTCGGCCGACGCCCTGGCCATCCTCAAACGCTACAACATCGAAGCGCCCAAAGCGGTCATCGACTGGCTGAACAACCCGGCCTCGGAAACCGTCAAGGCCGATGACCCGGCCCTGGCCCGCAGCCACTT > NC_002947/4891333‑4891626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |