| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,041,300 | T→A | 7.4% | intergenic (+230/‑432) | PP_5680 → / → PP_4447 | Cro/CI family transcriptional regulator/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,041,300 | 0 | T | A | 7.4% | 159.0 / 3.6 | 54 | intergenic (+230/‑432) | PP_5680/PP_4447 | Cro/CI family transcriptional regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (28/22); new base A (2/2); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
GTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTATCAATATCAGCCCCCCACCGAAAAGTCATT > NC_002947/5041154‑5041444 | gtTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGc < 1:848112/150‑1 (MQ=255) ttGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCg > 1:432550/1‑150 (MQ=255) gATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGt < 1:148884/150‑1 (MQ=255) cacaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCATAAGGCGGttagg < 1:1056099/150‑4 (MQ=255) gagGGTTAGGGCTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGCCCCCTTTGCTGAAGGTTCTGAAGCCCGCCCAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGc < 2:587534/150‑1 (MQ=255) ttAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTACGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGc > 1:1093522/1‑145 (MQ=255) ttAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGc < 2:1093522/145‑1 (MQ=255) ttAAGGCGTAGTTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGa > 2:861140/1‑150 (MQ=255) ttAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGa > 2:826502/1‑150 (MQ=255) tAAGGCGTACTTCTCTGATACCAACACCTCTGCGATGAGGACATGTCCTCTTCAGCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGaa < 1:388349/150‑1 (MQ=255) aGGCGTAATTGTCTGATACCAACACCTCTGCGAGGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTATCCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAgg < 1:453437/150‑1 (MQ=255) gCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGcc > 2:56631/1‑150 (MQ=255) tCTGATACCAACACCTCTGCTATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa < 1:56631/150‑1 (MQ=255) tCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa > 1:894905/1‑150 (MQ=255) cAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTccaacc > 1:929007/1‑150 (MQ=255) ctctGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAg > 1:436512/1‑124 (MQ=255) ctctGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAg < 2:436512/124‑1 (MQ=255) ctctGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCATAAGGCGGttagggg < 2:438660/103‑6 (MQ=255) ctctGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCATAAGGCGGttagggg > 1:438660/1‑98 (MQ=255) aTGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGACAAATAACTGTTTCTCTGCCTCCGAGACTAGAGCAAAGGCCGAGATCCGAGTCCAACCAATTCTATTTTTCGACCTGGc > 1:519742/1‑150 (MQ=255) gACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCt > 2:1128928/1‑150 (MQ=255) ctctTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGAc > 1:153890/1‑150 (MQ=255) tCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTa > 1:898083/1‑150 (MQ=255) ggCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTaga < 2:36123/150‑1 (MQ=255) ttAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGt < 2:76137/60‑1 (MQ=255) ttAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGt > 1:76137/1‑60 (MQ=255) gCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtct > 1:1093158/1‑60 (MQ=255) gCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtct < 2:1093158/60‑1 (MQ=255) cgccgAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCagg < 1:826502/150‑2 (MQ=255) cgccgAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCaga > 1:617068/1‑150 (MQ=255) cgAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGaa < 2:898083/150‑1 (MQ=255) agaagaGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGaaaa < 2:652075/150‑1 (MQ=255) gagCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGGGCTATCAGAGAAAAGTgg > 1:478818/1‑150 (MQ=255) agCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGt > 2:24260/1‑150 (MQ=255) agCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGt < 1:24260/150‑1 (MQ=255) ccAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTAt > 1:265246/1‑150 (MQ=255) ccccGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTATCAATATc < 2:617068/150‑1 (MQ=255) cccAAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTATCAATATCa > 2:665780/1‑150 (MQ=255) cGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa > 2:240487/1‑70 (MQ=255) cGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa < 1:240487/70‑1 (MQ=255) gCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGc > 1:581054/1‑96 (MQ=255) gCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGc < 2:581054/96‑1 (MQ=255) gACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGcc > 2:958835/1‑61 (MQ=255) gACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGcc < 1:958835/61‑1 (MQ=255) agagaaaCCGCCATAAGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTATCAATATCAGCCCCCCACCg > 1:534458/6‑150 (MQ=255) cccTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTATCAATATCAGCCCCCCACCGa > 1:108708/1‑150 (MQ=255) ccTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTATCAATATCAGCCCCCCACCGaa > 2:658912/1‑150 (MQ=255) cTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTATCAATATCAGCCCCCCACCGaaa > 1:670854/1‑150 (MQ=255) tAACCGCCTTATGGCGGTGTTTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTACCAGAGAAAAGTGGTATAAATATCAGCCCCCCACCGaaaa < 2:377679/150‑1 (MQ=255) ccGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTaga > 1:932679/1‑97 (MQ=255) ccGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTaga < 2:932679/97‑1 (MQ=255) ccTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTATCAATATCAGCCCCCCACCGAAAAGTCAtt > 1:385610/1‑150 (MQ=255) cTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGtgtg > 2:118860/1‑100 (MQ=255) cTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGtgtg < 1:118860/100‑1 (MQ=255) | GTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGAGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGTGCTATCAGAGAAAAGTGGTATCAATATCAGCCCCCCACCGAAAAGTCATT > NC_002947/5041154‑5041444 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |