| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,079,235 | C→A | 11.4% | noncoding (42/77 nt) | PP_t56 ← | tRNA‑Arg |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,079,235 | 0 | C | A | 11.4% | 92.9 / 3.3 | 35 | noncoding (42/77 nt) | PP_t56 | tRNA‑Arg |
| Reads supporting (aligned to +/- strand): ref base C (15/16); new base A (2/2); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01 | |||||||||||
CTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGC > NC_002947/5079090‑5079377 | cTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCAGCCTCAGCAGGATTCGAACCTGCGACCGCTCGGtt > 1:114907/1‑150 (MQ=255) acacCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa < 2:1003826/150‑1 (MQ=255) ttACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGACGCCGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa > 1:900202/1‑150 (MQ=255) cAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt < 1:296869/150‑1 (MQ=255) tAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAg < 1:842089/150‑1 (MQ=255) aGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGttt < 1:426404/150‑1 (MQ=255) aaacaaaAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACt > 2:558992/1‑150 (MQ=255) aaacaaaAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACt > 2:559068/1‑150 (MQ=255) acaaaAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTgg < 2:865055/150‑1 (MQ=255) acaaaAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTgg < 1:440589/150‑1 (MQ=255) aaaaaGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGTGGTTTTTAGCCAAGTTACCGCTGGCt > 1:421198/1‑150 (MQ=18) gCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt > 2:122662/1‑105 (MQ=255) gCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt < 1:122662/105‑1 (MQ=255) cccGCGCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATGGTAGTTTTAGAACAGGGTACCACTGGCTTACTGa > 1:826292/1‑150 (MQ=255) ccGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGaa > 2:644027/1‑150 (MQ=255) ccGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGGTTTAGACCAGGTTAACACTGGCTTACCGaa > 2:657817/1‑150 (MQ=255) gCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTAGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGc < 2:1059603/139‑1 (MQ=255) gCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTAGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGc < 2:1059404/139‑1 (MQ=255) gCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTAGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGc > 1:1059603/1‑139 (MQ=255) gCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTAGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGc > 1:1059404/1‑139 (MQ=255) tttttGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt > 2:1091149/1‑85 (MQ=255) tttttGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt < 1:1091149/85‑1 (MQ=255) tttttGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGaa > 1:920346/1‑150 (MQ=255) tttttGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGaa > 1:629315/1‑150 (MQ=255) ggggTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCa < 1:559068/150‑1 (MQ=255) ggggTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCa < 1:558992/150‑1 (MQ=255) gggTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCaa < 2:920346/150‑1 (MQ=255) gTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCtttt < 1:732325/130‑1 (MQ=255) gTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCtttt > 2:732325/1‑130 (MQ=255) gCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTg < 2:629315/150‑1 (MQ=255) ggACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGc < 2:900202/150‑1 (MQ=255) agcagGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTc > 2:693359/1‑150 (MQ=255) ggATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTcagca < 1:644027/150‑1 (MQ=255) gAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTc > 2:968917/1‑150 (MQ=255) gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGc < 1:693359/150‑1 (MQ=255) | CTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGC > NC_002947/5079090‑5079377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |