Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,115,762 C→A 11.5% R24R (CGG→CGT)  PP_4500 ← TrpH family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,115,7620CA11.5% 143.7 / 8.8 52R24R (CGG→CGT) PP_4500TrpH family protein
Reads supporting (aligned to +/- strand):  ref base C (22/24);  new base A (3/3);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GATGGTCGCGCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGCCATTGTGAAACAA  >  NC_002947/5115615‑5115909
                                                                                                                                                   |                                                                                                                                                   
gatggTCGCGCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCg                                                                                                                                                   <  1:335123/150‑1 (MQ=255)
 atggTCGCGCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCgg                                                                                                                                                  <  1:774354/150‑1 (MQ=255)
   ggTCGCGCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGGGCTCATGAGCACgggg                                                                                                                                                <  1:711520/150‑2 (MQ=255)
    gTCGCGCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGGGCTCATGAGCACggggt                                                                                                                                               <  1:468883/150‑3 (MQ=255)
      cgcgCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAAc                                                                                                                                             <  2:523806/150‑1 (MQ=255)
       gcgcCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGGGCTCATGAGCACggg                                                                                                                                                 >  2:724463/1‑145 (MQ=255)
       gcgcCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGGGCTCATGAGCACggg                                                                                                                                                 <  1:724463/145‑1 (MQ=255)
                 aCGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAg                                                                                                                                  >  1:584777/1‑150 (MQ=255)
                                  aCCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAAc                                                                                                                                             <  2:889274/122‑1 (MQ=255)
                                  aCCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAAc                                                                                                                                             >  1:889274/1‑122 (MQ=255)
                                     ccGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAg                                                                                                              <  2:584777/150‑1 (MQ=255)
                                             ccAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGcc                                                                                                                     <  2:312070/135‑1 (MQ=255)
                                             ccAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGcc                                                                                                                     >  1:312070/1‑135 (MQ=255)
                                                  gcATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGAc                                                                                                 >  1:196095/1‑150 (MQ=255)
                                                     tACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGt                                                                                              <  1:715938/150‑1 (MQ=255)
                                                      aCCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTg                                                                                             <  1:920760/150‑1 (MQ=255)
                                                        ccAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGa                                                                                                               <  1:145349/130‑1 (MQ=255)
                                                        ccAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGa                                                                                                               >  2:145349/1‑130 (MQ=255)
                                                        ccAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGcc                                                                                           >  2:381089/1‑149 (MQ=255)
                                                        ccAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCACGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCa                                                                                           >  1:962833/1‑150 (MQ=255)
                                                            gTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATc                                                                                       >  1:425644/1‑150 (MQ=255)
                                                              cAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCaa                                                                                     <  1:37536/150‑1 (MQ=255)
                                                               aaCGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCaa                                                                                                                                              >  1:727647/1‑92 (MQ=255)
                                                               aaCGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCaa                                                                                                                                              <  2:727647/92‑1 (MQ=255)
                                                                   cAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCATATCAACATTc                                                                                <  2:196095/150‑1 (MQ=255)
                                                                       ccTNGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATag                                                                            >  2:701646/1‑150 (MQ=255)
                                                                         tGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATagag                                                                          >  2:947817/1‑150 (MQ=255)
                                                                                 ttCNGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCtt                                                                  >  2:697589/1‑150 (MQ=255)
                                                                                         ggCCTTCGAGGGTGTCATGATCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGa                                                          <  2:1097934/150‑1 (MQ=255)
                                                                                            cTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATaga                                                                           >  1:1089517/1‑130 (MQ=255)
                                                                                            cTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATaga                                                                           <  2:1089517/130‑1 (MQ=255)
                                                                                             ttCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCt                                                             <  2:138132/143‑1 (MQ=255)
                                                                                             ttCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCt                                                             >  1:138132/1‑143 (MQ=255)
                                                                                                         aTGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGttatt                                          <  2:867811/150‑1 (MQ=255)
                                                                                                         aTGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGttatt                                          >  1:140110/1‑150 (MQ=255)
                                                                                                                 tcAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTc                                  >  1:365701/1‑150 (MQ=255)
                                                                                                                   aGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACAtt                                                                                 <  1:2402/101‑1 (MQ=255)
                                                                                                                   aGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACAtt                                                                                 >  2:2402/1‑101 (MQ=255)
                                                                                                                         aGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCg                          >  2:376379/1‑150 (MQ=255)
                                                                                                                         aGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCg                          >  1:390750/1‑150 (MQ=255)
                                                                                                                              ttGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCtgt                                                                                                    <  1:837084/71‑1 (MQ=255)
                                                                                                                              ttGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCtgt                                                                                                    >  2:837084/1‑71 (MQ=255)
                                                                                                                               tGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTg                    <  1:947817/150‑1 (MQ=255)
                                                                                                                                gCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGTGATAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTgg                   <  2:181424/150‑1 (MQ=255)
                                                                                                                                gCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTgg                   <  2:810013/150‑1 (MQ=255)
                                                                                                                                    cccGGGCTCATGAGCACGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGc               >  1:1063008/1‑150 (MQ=255)
                                                                                                                                       gggCTCATGAGCACGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGCCAt            >  2:935249/3‑150 (MQ=255)
                                                                                                                                           tCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGa                                                     <  1:857812/105‑1 (MQ=255)
                                                                                                                                           tCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGa                                                     >  2:857812/1‑105 (MQ=255)
                                                                                                                                           tCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGCCATtgtg        <  2:140110/150‑1 (MQ=255)
                                                                                                                                             aTGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGCCATTGTGaa      >  2:1045288/1‑150 (MQ=255)
                                                                                                                                                 gCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGCCATTGTGAAACaa  <  1:1045288/150‑1 (MQ=255)
                                                                                                                                                   ccGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGCCATTGTGa       >  1:1048047/1‑143 (MQ=255)
                                                                                                                                                   ccGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGCCATTGTGa       <  2:1048047/143‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATGGTCGCGCCGCCCCACGTGCAGGACAGCTCCACCCCGCTGACCCAGCGCATACCCAGGTCAACGCAGGCCTGGCGCGCTTCGGGCAGGCCTTCGAGGGTGTCATGGTCGGTCAGTGCCAGCGCTTGCACCCCGTGCTCATGAGCCCGGGCAACCAGTACCGAAGGCGACAGGGCGCCGTCGGAGGCCGTGCTGTGACAGTGCAGATCAACATTCATAGAGGAGCGCTTTCGCTGGATTCGATGTTTGTTATTATGCCGTCACATCCCGATTCTGGCTGCCATTGTGAAACAA  >  NC_002947/5115615‑5115909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑