| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,221,722 | T→C | 6.9% | V267A (GTC→GCC) | PP_4601 → | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,221,722 | 0 | T | C | 6.9% | 156.6 / 2.0 | 58 | V267A (GTC→GCC) | PP_4601 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (31/23); new base C (2/2); total (33/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01 | |||||||||||
GCGACTCGAGCTTCCAGCTTATCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGCCGGTCAGCTGCTTGA > NC_002947/5221584‑5221862 | gCGACTCGAGCTTCCAGCTTATCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGgctgctgc > 1:925721/1‑150 (MQ=255) aCTCGAGCTTCCAGCTTATCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCtgctgctgc > 2:589551/1‑150 (MQ=255) tCGAGCTTCCAGCTTATCCAGGCCATCGACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCAGCGAACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTgcgc > 1:617319/1‑150 (MQ=255) tCGAGCTTCCAGCTTATCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTgcgc < 1:871965/150‑1 (MQ=255) tCGAGCTTACAGCTTATCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTgcgc > 1:997181/1‑150 (MQ=255) aGCTTATCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGc > 1:495733/1‑150 (MQ=255) ggttAACCAGCCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGcc < 1:1036677/148‑1 (MQ=255) gCTTATCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGcc > 1:77013/1‑150 (MQ=255) tCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCt > 2:26812/1‑150 (MQ=255) tCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGACCGGgctgt > 2:715194/1‑125 (MQ=255) tCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGACCGGgctgt < 1:715194/126‑2 (MQ=255) aGGNCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTAACGGGGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCgg > 2:700047/1‑150 (MQ=255) aGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCtgctgctgc < 1:334414/129‑1 (MQ=255) aGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCtgctgctgc > 2:334414/1‑129 (MQ=255) aTCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGAtt > 2:673629/1‑150 (MQ=255) cgccGGCATATGCTGCGCGATCATGTCACTGGACTGCGGCCTGGAAGAACTCAGCGAGCACCTGCGCATGGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGa < 2:418771/148‑1 (MQ=255) ccGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGc > 2:33889/1‑150 (MQ=255) cGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGc > 2:817476/1‑124 (MQ=255) cGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGc < 1:817476/124‑1 (MQ=255) ggCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCaa < 1:26812/150‑1 (MQ=255) ggCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCaa > 2:48069/1‑150 (MQ=255) ggCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACATGCGCATCGTAACGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAACaa > 2:48103/1‑150 (MQ=255) gCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAAt < 2:495733/150‑1 (MQ=255) tGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCggg > 1:757743/1‑150 (MQ=255) tGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCGCGGCGATTGCCTAGCAATGCTTTGCCGAGCCGCGGGGGCTGt > 2:68143/1‑150 (MQ=255) tGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAg < 1:700047/150‑1 (MQ=255) ggCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGcc > 2:598540/1‑150 (MQ=255) tGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCt < 2:418839/150‑1 (MQ=255) gAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGa > 2:981463/1‑150 (MQ=255) tCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATcggcgg > 1:538509/1‑150 (MQ=255) tCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATcggcgg > 1:211807/1‑150 (MQ=255) cAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCCAACAGTAC‑TTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGt < 1:68143/150‑1 (MQ=255) cacgcgcgcATCGTTCGGTTGGCAGACGCGGGACTCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGACGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTgg < 2:286646/145‑1 (MQ=255) cTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTg < 1:395863/85‑1 (MQ=255) cTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTg > 2:395863/1‑85 (MQ=255) cTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCggg > 1:1105472/1‑112 (MQ=255) cTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCggg < 2:1105472/112‑1 (MQ=255) aTCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCAGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTgg > 1:262415/1‑150 (MQ=255) ccGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCgcggc < 1:33889/150‑1 (MQ=255) ccGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCgcggc < 1:673629/150‑1 (MQ=255) ggCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTaaa < 1:598540/150‑1 (MQ=255) cAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGc > 1:160249/1‑150 (MQ=255) aGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGcc > 1:942869/1‑150 (MQ=255) gccgccATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGCCGGTc < 2:160249/150‑1 (MQ=255) gccgccATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGCCGGTc > 1:40660/1‑150 (MQ=255) cgccATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAg < 2:662610/79‑1 (MQ=255) cgccATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAg > 1:662610/1‑79 (MQ=255) gccATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGCCGGTCAgc < 2:757743/150‑1 (MQ=255) ccATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGc < 2:435530/120‑1 (MQ=255) ccATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGc > 1:435530/1‑120 (MQ=255) cATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGCCGGTCAgctg > 1:212296/1‑150 (MQ=255) tCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGCCGGTCAgctgct < 2:77013/150‑1 (MQ=255) cc‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTGTCTATGATCGGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGCCGGTCAGCTGCtt < 2:997181/150‑1 (MQ=255) gcaGCCCGACCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGcc < 1:935764/135‑1 (MQ=255) gcaGCCCGACCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGcc > 2:935764/2‑136 (MQ=255) acaGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGCCGGTCAGCTGCTTGa > 1:793642/1‑150 (MQ=255) ccGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCaa < 2:526964/54‑1 (MQ=255) ccGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCaa > 1:526964/1‑54 (MQ=255) | GCGACTCGAGCTTCCAGCTTATCCAGGCCATCAACACCGGCATATGCTGCGCGATCATGCCACTGGACTGCGGCCTGGAAGACCTCAGCGAGCACCTGCGCATCGTACCGGTGGCAGACGCCGCCATCC‑ACAGCCCGGTCGGGCTGCTGCTGCGCCGCAGCGAGCCGCGCTCGGCGATTGCCGAGCAATGCTTTGCCGAGGCGCGGGGGCTGTTTCAGGCCAGCTGATCGGCGGTAGCCTGGCGGTACTGGCGCGGCGTAAAGCCGGTCAGCTGCTTGA > NC_002947/5221584‑5221862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |