| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 467,101 | A→G | 10.0% | intergenic (‑31/+22) | PP_5445 ← / ← PP_t71 | hypothetical protein/tRNA‑Met |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 467,101 | 0 | A | G | 10.0% | 160.3 / 7.5 | 60 | intergenic (‑31/+22) | PP_5445/PP_t71 | hypothetical protein/tRNA‑Met |
| Reads supporting (aligned to +/- strand): ref base A (24/30); new base G (3/3); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
GGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATC > NC_002947/466963‑467245 | ggTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCt > 2:854486/1‑150 (MQ=255) tGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCttt < 1:946505/150‑1 (MQ=255) tGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTAAGCGCCCCTGACCAGTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCttt > 1:977447/1‑150 (MQ=255) gACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCtttt < 1:152426/150‑2 (MQ=255) ccGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc < 2:498212/150‑1 (MQ=255) ccGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc < 2:341643/150‑1 (MQ=255) gCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTa > 2:945212/1‑150 (MQ=255) ttcttGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATc < 1:854486/150‑1 (MQ=255) cTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCcac > 1:257266/1‑150 (MQ=255) ttCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGa < 1:486936/150‑1 (MQ=255) ttCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGa < 2:257266/150‑1 (MQ=255) cGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACt < 2:544931/150‑1 (MQ=255) cTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTAGGCCCACACGGACTCg > 1:1036796/1‑150 (MQ=255) agaTACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAAc < 1:107324/150‑1 (MQ=255) aCCGTTTTAGCGCTACTTCCATGTTCATTCGCTCGACGCCGCTGCGCTTGATATCCTCGCCCCTGACCATTTTTTTCGTTCGTGCTTGATAAATCCGCTCAATCTCGCAGCTTTCTCGTATCTCGTGGGCCCACGCGGACTCAAACCGTg < 2:508707/150‑1 (MQ=255) aCCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGAGCGTCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTg > 1:508707/1‑150 (MQ=255) ccGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTgg > 2:802768/1‑150 (MQ=255) ttttAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATcgcgcg > 2:750265/1‑105 (MQ=255) ttttAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATcgcgcg < 1:750265/105‑1 (MQ=255) tAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCaaa < 1:789071/150‑1 (MQ=255) aGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAg < 2:21250/150‑1 (MQ=255) gcgcTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc > 2:1109976/1‑108 (MQ=255) gcgcTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc < 1:1109976/108‑1 (MQ=255) tACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTa < 1:712510/150‑1 (MQ=255) ttCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGCTGCCTGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGa < 1:336165/150‑1 (MQ=255) ttCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGa > 2:529056/1‑150 (MQ=255) ccAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGt > 2:848204/1‑150 (MQ=255) cAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCtt > 1:177639/1‑94 (MQ=255) cAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCtt < 2:177639/94‑1 (MQ=255) ggTCATGCGCTCGGCGCCGCTACGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCctc > 1:1037103/1‑150 (MQ=255) cTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAAc < 1:513354/150‑1 (MQ=255) gcgcCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCaa > 1:620583/1‑149 (MQ=255) gcgcCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCaa < 2:620583/149‑1 (MQ=255) ccgcTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGa < 1:848204/150‑1 (MQ=255) cgctgcgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAg < 1:529056/150‑1 (MQ=255) gctgcgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGc > 2:1053258/1‑150 (MQ=255) gctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCAGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTAt < 2:1052891/119‑1 (MQ=255) gctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCAGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTAt > 1:1052891/1‑119 (MQ=255) tgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCAAACCGTCGCCCAAATGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGc > 1:63174/1‑150 (MQ=255) gATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCaaa < 1:104827/108‑1 (MQ=255) gATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCaaa > 2:104827/1‑108 (MQ=255) gATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAg < 1:687789/140‑1 (MQ=255) gATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAg > 2:687789/1‑140 (MQ=255) cgcgCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtggtgg < 1:859162/73‑1 (MQ=255) cgcgCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtggtgg > 2:859162/1‑73 (MQ=255) gcCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGt < 1:680577/150‑1 (MQ=255) ccTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTggg > 2:362959/1‑150 (MQ=255) ccctttatttCTTGCGGGCATGAAAAAGCCGCGCAATAACCTGTCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTTTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGAt < 1:851345/147‑1 (MQ=255) cATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGAtt > 2:1006843/1‑150 (MQ=255) tCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAg < 1:802768/150‑1 (MQ=255) gggCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTAGCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTc < 1:560333/150‑1 (MQ=255) aTGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCAc < 1:945212/150‑1 (MQ=255) aaaGCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAAAGAAGGTTTTGCACCtata > 2:237022/1‑147 (MQ=255) gCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCa > 2:839469/1‑150 (MQ=255) gCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGtt > 2:728036/1‑136 (MQ=255) gCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGtt > 2:728150/1‑136 (MQ=255) gCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGtt < 1:728036/136‑1 (MQ=255) gCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGtt < 1:728150/136‑1 (MQ=255) ccGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCAt < 2:234882/150‑1 (MQ=255) cgcgCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATc > 2:772313/1‑150 (MQ=255) | GGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATC > NC_002947/466963‑467245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |