Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,382,317 A→T 7.3% E124D (GAA→GAT)  smpB → SsrA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,382,3170AT 7.3% 167.3 / 3.4 55E124D (GAA→GAT) smpBSsrA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (22/29);  new base T (2/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CACCCACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGGG  >  NC_002947/5382170‑5382461
                                                                                                                                                   |                                                                                                                                                
cACCCACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAAt                                                                                                                                                >  2:904627/1‑150 (MQ=255)
   ccACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGc                                                                                                                                             >  1:645238/1‑150 (MQ=255)
         cATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATTTCGCAcntga                                                                                                                                       <  2:697819/150‑5 (MQ=255)
              ccGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaagg                                                                                                                                  >  2:127847/1‑150 (MQ=255)
               cGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGAGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaaggg                                                                                                                                 <  2:375482/150‑1 (MQ=255)
                gACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaagggc                                                                                                                                <  1:904627/150‑1 (MQ=255)
                     tACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaa                                                                                                                           >  1:637637/1‑150 (MQ=255)
                      acacGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaag                                                                                                                          <  1:308128/150‑1 (MQ=255)
                      acacGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaag                                                                                                                          <  2:637637/150‑1 (MQ=255)
                        acGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATTTCGCACttga                                                                                                                                       >  2:36855/1‑132 (MQ=255)
                        acGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATTTCGCACttga                                                                                                                                       <  1:36855/135‑4 (MQ=255)
                             cccGCAAGCTGCTGCTGAACAAGCGCTGGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCg                                                                                                                   >  1:704798/1‑150 (MQ=255)
                                   aGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAgc                                                                                                             <  2:201217/150‑1 (MQ=255)
                                      tgctgctgAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGcgcg                                                                                                          >  2:575435/1‑150 (MQ=255)
                                       gctgctgAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGa                                                                                                         >  2:957213/1‑150 (MQ=255)
                                             gAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCAt                                                                                                   >  1:1018235/1‑150 (MQ=255)
                                              aaCAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATg                                                                                                  >  2:475020/1‑150 (MQ=255)
                                               aCAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGTTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATgc                                                                                                 <  1:700731/150‑1 (MQ=255)
                                                cAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATgcg                                                                                                >  2:1093978/1‑150 (MQ=255)
                                                    cgcgAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGaa                                                                                            <  1:538367/150‑1 (MQ=255)
                                                     gcgAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGAGCTGTACTGTAGGCAGCAGCTGCTACACTCCGAAAGCGCACTGAGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACGATGCGCGaat                                                                                           >  1:78438/1‑149 (MQ=255)
                                                             gAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTcc                                                                                   >  1:894811/1‑150 (MQ=255)
                                                               gcgcGTGGAAGCGGCCGTGGCGTAAAAAGGCCACACCTGTGTGGCTTTGGTGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCaa                                                                                 <  2:581115/150‑1 (MQ=255)
                                                               gcgcGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCaa                                                                                 <  2:1018235/150‑1 (MQ=255)
                                                                              cGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCg                                                                  <  1:795821/150‑1 (MQ=255)
                                                                                    gcAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCtgt                                                            >  1:877595/1‑150 (MQ=255)
                                                                                              tACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAANAAg                                                  <  2:704798/150‑1 (MQ=255)
                                                                                               acacCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAgg                                                 <  1:653801/150‑1 (MQ=255)
                                                                                               acacCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAgg                                                 <  2:894811/150‑1 (MQ=255)
                                                                                                cacCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAggg                                                <  1:957213/150‑1 (MQ=255)
                                                                                                   cTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgg                                                                                                                                        <  1:1058074/59‑1 (MQ=255)
                                                                                                   cTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgg                                                                                                                                        >  2:1058074/1‑59 (MQ=255)
                                                                                                   cTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCaa                                             <  2:645238/150‑1 (MQ=255)
                                                                                                    tgtgtgGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaagg                                                                                                                                  >  2:968607/1‑64 (MQ=255)
                                                                                                    tgtgtgGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaagg                                                                                                                                  <  1:968607/64‑1 (MQ=255)
                                                                                                         ggCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGAGCGCGGTCCAGTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGCAaga                                       <  1:922517/150‑1 (MQ=255)
                                                                                                          gCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAagag                                      <  1:1093978/150‑1 (MQ=255)
                                                                                                           cTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGt                                     >  2:887014/1‑150 (MQ=255)
                                                                                                                acgcTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGAc                                                                                                                 <  2:385894/68‑1 (MQ=255)
                                                                                                                acgcTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGAc                                                                                                                 >  1:385894/2‑69 (MQ=255)
                                                                                                                  gcTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAactact                              >  1:366911/1‑150 (MQ=255)
                                                                                                                   cTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTActc                             <  1:127847/150‑1 (MQ=255)
                                                                                                                    tGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACTAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTActct                            <  1:575435/150‑1 (MQ=255)
                                                                                                                                   aCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGc             <  2:877595/150‑1 (MQ=255)
                                                                                                                                      tGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGCAAGAGTAACTACGCTTCAACCTGTTCCGGCctc          <  1:471362/150‑1 (MQ=255)
                                                                                                                                       gATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCctct         >  1:1009175/1‑150 (MQ=255)
                                                                                                                                       gATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCctct         <  1:765846/150‑1 (MQ=255)
                                                                                                                                          ccaGTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGAGAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTcg      >  1:790042/3‑150 (MQ=255)
                                                                                                                                             gTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCgg   <  2:78438/150‑1 (MQ=255)
                                                                                                                                             gTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTACCGGCCTCTTCGCgg   >  2:517417/1‑150 (MQ=255)
                                                                                                                                              tGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCggg  <  2:366911/150‑1 (MQ=255)
                                                                                                                                              tGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCgg   <  1:1001144/149‑1 (MQ=255)
                                                                                                                                              tGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCgg   >  2:1001144/1‑149 (MQ=255)
                                                                                                                                                cGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAaga                                       <  1:48655/111‑1 (MQ=255)
                                                                                                                                                cGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAaga                                       >  2:48655/1‑111 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CACCCACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGGG  >  NC_002947/5382170‑5382461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑