Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,388,931 T→G 8.6% L798R (CTG→CGG) ‡ dld2 → D‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,388,9310TG 8.6% 150.5 / 7.4 60L798R (CTG→CGG) ‡dld2D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (28/26);  new base G (3/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

CCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGG  >  NC_002947/5388785‑5389071
                                                                                                                                                  |                                                                                                                                            
ccTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGt                                                                                                                                           <  1:1063549/150‑1 (MQ=255)
   gtgCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACACGCCCCTGCACCCTGCGCCTGGTGCa                                                                                                                                        <  1:337199/150‑1 (MQ=255)
     gctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCACCAGGCGCAgg                                                                                                                                      <  2:638768/150‑1 (MQ=255)
              aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCg                                                                                                                             >  2:833284/1‑150 (MQ=255)
                      gCCACCAAGGGCTACCCCGAGCAGGCCGAACTCAAACGCCAGGAGCTGATCACCGCCCTGCTGCCCGCCAGCCGCGGCGGCCACGACCCGATCTATTGCACCACAAGCCCCTGCACCCTGCGCCTGGTGCGGGACCTGAGCGACACCCgg                                                                                                                     >  2:624363/1‑150 (MQ=255)
                         tCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                  <  2:176082/150‑1 (MQ=255)
                         tCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                  >  1:320972/1‑150 (MQ=255)
                          ccAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAggg                                                                                                                                     >  1:858235/1‑129 (MQ=255)
                          ccAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAggg                                                                                                                                     <  2:858235/130‑2 (MQ=255)
                           cAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                >  1:227486/1‑150 (MQ=255)
                           cAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                >  1:63981/1‑150 (MQ=255)
                              gggCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGTGTGACCCGGGTGGACCt                                                                                                             <  2:1123642/150‑1 (MQ=255)
                                gCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGTACCTGt                                                                                                           <  2:320972/150‑1 (MQ=255)
                                 cTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTa                                                                                                          <  1:546258/150‑1 (MQ=255)
                                  tACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTAc                                                                                                         <  1:278378/150‑1 (MQ=255)
                                               gcGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCt                                                                                            <  2:892233/149‑1 (MQ=255)
                                                 gAACCCAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCAGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTc                                                                                          >  2:683812/1‑150 (MQ=255)
                                                    cacaAACGCCAGGAGCTAATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATc                                                                                       <  1:334969/150‑1 (MQ=255)
                                                     acaAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATcc                                                                                      >  1:166840/1‑150 (MQ=255)
                                                       aaaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAgg                                                                                                                                      <  2:380935/100‑1 (MQ=255)
                                                       aaaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAgg                                                                                                                                      >  1:380935/1‑100 (MQ=255)
                                                           gCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcaccc                                                                                <  1:360823/150‑1 (MQ=255)
                                                             cAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGTGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGGCCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                              >  2:1120524/1‑150 (MQ=255)
                                                             cAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGGCCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                              >  2:1120301/1‑150 (MQ=255)
                                                               ggAGCTGCTCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGTGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                            <  2:512270/150‑1 (MQ=255)
                                                               ggAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGAGCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                            >  1:304358/1‑150 (MQ=255)
                                                                    tGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcg                                                                       >  1:296747/1‑150 (MQ=255)
                                                                       tCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgacc                                                                    >  1:99123/1‑150 (MQ=255)
                                                                           cGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATGCGCACCCACCTGCTCGACCGACt                                                                >  1:728091/1‑150 (MQ=255)
                                                                           cGCCATGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACt                                                                >  2:1081951/1‑150 (MQ=255)
                                                                            gCCCTGCTGCACGCCAGCCGCGCCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGNCTg                                                               <  2:694445/150‑1 (MQ=255)
                                                                                   tgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                        >  1:865661/1‑150 (MQ=255)
                                                                                   tgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                        <  2:817054/150‑1 (MQ=255)
                                                                                             ccGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGa                                              <  1:422251/150‑1 (MQ=255)
                                                                                             ccGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGa                                              <  2:227486/150‑1 (MQ=255)
                                                                                                  gcggcCTCGACCCGATCTATTGCGACTATATCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAc                                         >  1:30116/1‑150 (MQ=255)
                                                                                                   cggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGTAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAcc                                        <  1:882472/150‑1 (MQ=255)
                                                                                                   cggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAccccc                                                   <  1:817373/139‑1 (MQ=255)
                                                                                                   cggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAccccc                                                   >  2:817373/1‑139 (MQ=255)
                                                                                                        tCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAg                                   >  1:695492/1‑150 (MQ=255)
                                                                                                         cGACCCGATCTATTGCGACAGAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGc                                  <  2:265071/150‑1 (MQ=255)
                                                                                                          gACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCt                                 >  2:1054711/1‑150 (MQ=255)
                                                                                                            cccGCTCTATTGCGACACAAGCCGCTGCACTCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgt                               <  2:63981/150‑1 (MQ=255)
                                                                                                              cGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTCCGGCCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGc                             >  1:352076/1‑150 (MQ=255)
                                                                                                               gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCa                            >  2:629838/1‑150 (MQ=255)
                                                                                                                  cTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCGTCGTCCGCACCCACCTGCTCGACCGACTGGAGTTCCCCCCCCAGGACGAACCGGTAGCTGTGCACGt                         >  1:1093932/1‑150 (MQ=255)
                                                                                                                   tATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTg                        >  2:1061769/1‑150 (MQ=255)
                                                                                                                    aTTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgc                                                                                               >  2:544546/1‑78 (MQ=255)
                                                                                                                    aTTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgc                                                                                               <  1:544546/78‑1 (MQ=255)
                                                                                                                       gCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                              >  2:645498/1‑92 (MQ=255)
                                                                                                                       gCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                              <  1:645498/92‑1 (MQ=255)
                                                                                                                          acacAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgca                 <  2:865661/150‑1 (MQ=255)
                                                                                                                            acaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgt                               <  2:124833/134‑1 (MQ=255)
                                                                                                                            acaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgt                               >  1:124833/1‑134 (MQ=255)
                                                                                                                             tcaggtccTGCACCAGGCGCAGGGTGCAGGACCTGAGCGCCACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGGCGGACCGGGAGCTGTGCACGGGACCTgcagca              >  2:1088910/7‑150 (MQ=255)
                                                                                                                             caAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcagca              >  1:455617/1‑150 (MQ=255)
                                                                                                                                 ccccTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcaccca          <  2:296747/150‑1 (MQ=255)
                                                                                                                                   ccTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagc        >  2:389651/1‑150 (MQ=255)
                                                                                                                                        aCCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTg   <  2:352076/150‑1 (MQ=255)
                                                                                                                                         cccTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTgg  <  2:689568/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
CCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGG  >  NC_002947/5388785‑5389071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑