Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,427,625 G→C 9.8% L176V (CTG→GTG)  rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,427,6250GC 9.8% 121.6 / 5.5 41L176V (CTG→GTG) rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base G (18/19);  new base C (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

GGCACCAGCCACTGCTTGACGCTGGCGGCCGTGGCGTTGCGCGGGCTCACTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCA  >  NC_002947/5427477‑5427768
                                                                                                                                                    |                                                                                                                                               
ggCACCAGCCACTGCTTGACGCTGGCGGCCGTGGCGTTGCGCGGGCTCACTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGt                                                                                                                                                <  1:420830/150‑1 (MQ=255)
     cAGCCACTGCTTGCCGATGGCGGCCGTGGCGTTGCGCGGGCTCACTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCcgc                                                                                                                                           >  2:831295/1‑150 (MQ=255)
             gCTTGACGCTGGCGGCCGTGGCGTTGCGCGGGCTCACTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCg                                                                                                                                   >  2:235462/1‑150 (MQ=255)
                          ggcCGTGGCGTTGCGCGGGCTCACTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTc                                                                                                                      >  2:818809/1‑150 (MQ=255)
                                ggCGTTGCGCGGGCTCACTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACt                                                                                                                                <  1:437567/134‑1 (MQ=255)
                                ggCGTTGCGCGGGCTCACTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACt                                                                                                                                >  2:437567/1‑134 (MQ=255)
                                   gTTGCGCGGGCTCACTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGcc                                                                                                             >  1:1017166/1‑150 (MQ=255)
                                                aCTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATg                                                                                                <  2:1126637/150‑1 (MQ=255)
                                                  ttCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCg                                                                                              >  1:490083/1‑150 (MQ=255)
                                                      aTACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCg                                                                                          >  1:317287/1‑150 (MQ=255)
                                                      aTACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCACTGCcgcgcaa                                                                                                                                       >  1:379927/1‑102 (MQ=255)
                                                      aTACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCACTGCcgcgcaa                                                                                                                                       <  2:379927/105‑4 (MQ=255)
                                                        aCTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGc                                                                                        <  1:235462/150‑1 (MQ=255)
                                                          tGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCtt                                                                                      <  1:595839/150‑1 (MQ=255)
                                                            aGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGt                                                                                                                                                <  1:291826/90‑1 (MQ=255)
                                                            aGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGt                                                                                                                                                >  2:291826/1‑90 (MQ=255)
                                                              ggggTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATc                                                                                  <  1:818809/150‑1 (MQ=255)
                                                                ggTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCa                                                                                <  2:384553/150‑1 (MQ=255)
                                                                      tCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGAACAAGGTGGAGAAAAACTGCAGCGGCTGGCGCTTGTGCGGCAGTCCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCa                                                                          >  1:22303/1‑150 (MQ=255)
                                                                        aGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAgg                                                                        <  2:107926/150‑1 (MQ=255)
                                                                            ccAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCt                                                                    >  1:635620/1‑150 (MQ=255)
                                                                                 cccGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCAcc                                                               >  2:312078/1‑150 (MQ=255)
                                                                                  ccGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCt                                                              <  2:1017166/150‑1 (MQ=255)
                                                                                   cGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTg                                                             <  1:672652/150‑1 (MQ=255)
                                                                                   cGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTg                                                             >  2:672652/1‑150 (MQ=255)
                                                                                    gCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCCCCtca                                                            <  1:715186/150‑3 (MQ=255)
                                                                                          aTGCGAATCTGGTCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAAc                                                      <  2:21148/150‑1 (MQ=255)
                                                                                                     aTCGGAGAAGGTGGAGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTc                                                                                           <  1:1025306/102‑1 (MQ=255)
                                                                                                     aTCGGAGAAGGTGGAGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTc                                                                                           >  2:1025306/1‑102 (MQ=255)
                                                                                                      tCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTGGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTc                                          >  1:1111398/1‑150 (MQ=255)
                                                                                                         gagaAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGc                                       >  2:760714/1‑150 (MQ=255)
                                                                                                                          cagcagCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTTCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCaa                      >  1:779386/1‑150 (MQ=255)
                                                                                                                                       cgcTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTa         <  1:831295/150‑1 (MQ=255)
                                                                                                                                        gtgtacgcgGCACTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGtt                                                          >  1:114672/6‑100 (MQ=255)
                                                                                                                                        gtgtacgcgGCACTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGtt                                                          <  2:114672/95‑1 (MQ=255)
                                                                                                                                        gcTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCt                                                                    >  1:350209/1‑90 (MQ=255)
                                                                                                                                        gcTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCt                                                                    <  2:350209/90‑1 (MQ=255)
                                                                                                                                          ttGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGcc      <  2:490083/150‑1 (MQ=255)
                                                                                                                                           tGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGcca     <  2:755313/150‑1 (MQ=255)
                                                                                                                                           ggcgcGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGcca     <  2:721316/149‑1 (MQ=255)
                                                                                                                                              gcgGCAGTGCCGCGTACGCCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGccacca  >  2:73845/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGCACCAGCCACTGCTTGACGCTGGCGGCCGTGGCGTTGCGCGGGCTCACTTCGATACTGAGGGGGTCATTCAGCGCCAGCCCCGCCAGCATGCGAATCTGATCGGAGAAGGTGGCGGAAAACAGCAGCGTCTGGCGCTTGCGCGGCAGTGCCGCGTACACCGACTGCAGCTCTTCGGCAAAGCCCAGGTCAAGCATGCGGTCGGCTTCATCCAGCACCAGGGTCTGCACCTGGTTGAACTTCACGGCGTTCTGCCGGAACAGGTCGAGCAAGCGGCCAGGGGTAGCCACCA  >  NC_002947/5427477‑5427768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑